{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:22:46Z","timestamp":1777922566138,"version":"3.51.4"},"reference-count":0,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2007,9,1]],"date-time":"2007-09-01T00:00:00Z","timestamp":1188604800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2007,9]]},"abstract":"\n The presence of\n van<\/jats:italic>\n A-containing\n Enterococcus faecium<\/jats:italic>\n isolates was demonstrated in two of 67 fecal samples (3%) of healthy wild boars recovered in Portugal. One isolate per sample was studied further (\n E. faecium<\/jats:italic>\n PG5V and PG48V). The mechanisms of resistance for other antibiotics were analyzed in these two vancomycin-resistant isolates, as well as the presence of genes related with virulence factors. The\n tet<\/jats:italic>\n (M),\n tet<\/jats:italic>\n (L), and\n erm<\/jats:italic>\n (B) genes, associated with tetracycline or erythromycin resistance, were identified in both isolates, and the presence of\n aph<\/jats:italic>\n (3\u2032)-IIIa gene (associated with kanamycin resistance) was detected in one of them.\n E. faecium<\/jats:italic>\n PG48V exhibited an ampicillin minimum inhibitory concentration (MIC) of 64\n \u03bc<\/jats:italic>\n g\/ml and showed eight amino acid substitutions in the carboxy-terminal end of the PBP5 protein (461Q \u2192 K, 470H \u2192 Q, 485M \u2192 A, 496N \u2192 K, 499A \u2192 T, 525E \u2192 D, 586V \u2192 L, and 629E \u2192 V) with respect to the reference sequence (GenBank accession number X84860). The\n purK-6<\/jats:italic>\n allele was identified in isolate PG48V, which also harbored the\n cyl<\/jats:italic>\n L\n L<\/jats:sub>\n L\n S<\/jats:sub>\n ,\n entA<\/jats:italic>\n , and\n entB<\/jats:italic>\n genes, encoding cytolysin, enterocin A, and enterocin B, respectively. The\n purK-3<\/jats:italic>\n allele was found in the PG5V isolate. The two\n van<\/jats:italic>\n A-containing isolates harbored the Tn\n 916<\/jats:italic>\n \/Tn\n 1545<\/jats:italic>\n -like mobile element, and the Tn\n 5397<\/jats:italic>\n -like transposon was also found in isolate PG48V. The intestinal tract of wild boars could be a reservoir of\n van<\/jats:italic>\n A-containing enterococci.\n <\/jats:p>","DOI":"10.1089\/mdr.2007.759","type":"journal-article","created":{"date-parts":[[2007,10,21]],"date-time":"2007-10-21T11:12:25Z","timestamp":1192965145000},"page":"151-156","source":"Crossref","is-referenced-by-count":25,"title":["Characterization of\n van<\/i>\n A-Containing\n Enterococcus faecium<\/i>\n Isolates Carrying Tn\n 5397<\/i>\n -Like and Tn\n 916<\/i>\n \/Tn\n 1545<\/i>\n -Like Transposons in Wild Boars (\n Sus Scrofa<\/i>\n )"],"prefix":"10.1177","volume":"13","author":[{"given":"Patricia","family":"Poeta","sequence":"first","affiliation":[{"name":"University of Tr\u00e1s-os-Montes and Alto Douro, Veterinary Science Department, Vila Real, Portugal."},{"name":"Centre of Studies of Animal and Veterinary Sciences, Vila Real, Portugal."},{"name":"\u00c1rea Bioqu\u00edmica y Biolog\u00eda Molecular, Universidad de La Rioja, Logro\u00f1o, Spain."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela","family":"Costa","sequence":"additional","affiliation":[{"name":"University of Tr\u00e1s-os-Montes and Alto Douro, Veterinary Science Department, Vila Real, Portugal."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gilberto","family":"Igrejas","sequence":"additional","affiliation":[{"name":"Centre of Genetics and Biotechnology-University of Tr\u00e1s-os-Montes and Alto Douro\/Institute for Biotechnology and Bioengineering, Vila Real, Portugal."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatriz","family":"Rojo-Bezares","sequence":"additional","affiliation":[{"name":"\u00c1rea Bioqu\u00edmica y Biolog\u00eda Molecular, Universidad de La Rioja, Logro\u00f1o, Spain."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yolanda","family":"S\u00e1enz","sequence":"additional","affiliation":[{"name":"\u00c1rea Bioqu\u00edmica y Biolog\u00eda Molecular, Universidad de La Rioja, Logro\u00f1o, Spain."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Myriam","family":"Zarazaga","sequence":"additional","affiliation":[{"name":"\u00c1rea Bioqu\u00edmica y Biolog\u00eda Molecular, Universidad de La Rioja, Logro\u00f1o, Spain."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernanda","family":"Ruiz-Larrea","sequence":"additional","affiliation":[{"name":"\u00c1rea Bioqu\u00edmica y Biolog\u00eda Molecular, Universidad de La Rioja, Logro\u00f1o, Spain."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"University of Tr\u00e1s-os-Montes and Alto Douro, Veterinary Science Department, Vila Real, Portugal."},{"name":"Centre of Studies of Animal and Veterinary Sciences, Vila Real, Portugal."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmen","family":"Torres","sequence":"additional","affiliation":[{"name":"\u00c1rea Bioqu\u00edmica y Biolog\u00eda Molecular, Universidad de La Rioja, Logro\u00f1o, Spain."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2007,9]]},"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2007.759","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2007.759","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:24:17Z","timestamp":1777667057000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/mdr.2007.759"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,9]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2007,9]]}},"alternative-id":["10.1089\/mdr.2007.759"],"URL":"https:\/\/doi.org\/10.1089\/mdr.2007.759","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,9]]}}}