{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:17:47Z","timestamp":1791353867296,"version":"4.3.1"},"reference-count":0,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2008,6,1]],"date-time":"2008-06-01T00:00:00Z","timestamp":1212278400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2008,6]]},"abstract":"<jats:p>\n                    <jats:italic toggle=\"yes\">Salmonella<\/jats:italic>\n                    Genomic Island-1 (SGI-1) harbors a cluster of genes encoding multidrug resistance (MDR). SGI-1 is horizontally transmissible and is therefore of significant public health concern. This study presents two novel realtime PCRs detecting three SGI-1 protein-coding genes and a SGI-1 fingerprinting assay. These assays were applied to 445 European enterobacterial isolates. Results from real-time PCRs were comparable to those obtained from gelbased PCRs used for the detection of SGI-1, but were rapid to perform and suitable for large-scale screening. Furthermore, real-time PCRs also detected SGI-1 even when only part of the island was present in bacterial isolates. No trace of SGI-1 was detected in isolates other than\n                    <jats:italic toggle=\"yes\">Salmonella enterica<\/jats:italic>\n                    . The fingerprints showed that regions of SGI-1 outside the MDR region exhibited genomic variations between isolates. In conclusion, the realtime PCRs described here are suitable for the detection of SGI-1 in bacterial isolates. Further studies are necessary to elucidate divergence in its non-MDR region.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2008.0812","type":"journal-article","created":{"date-parts":[[2008,5,23]],"date-time":"2008-05-23T10:17:08Z","timestamp":1211537828000},"page":"79-92","source":"Crossref","is-referenced-by-count":18,"title":["Real-Time PCRs and Fingerprinting Assays for the Detection and Characterization of\n                    <i>Salmonella<\/i>\n                    Genomic Island-1 Encoding Multidrug Resistance: Application to 445 European Isolates of\n                    <i>Salmonella, Escherichia coli, Shigella<\/i>\n                    , and\n                    <i>Proteus<\/i>"],"prefix":"10.1177","volume":"14","author":[{"given":"Corinne F.L.","family":"Amar","sequence":"first","affiliation":[{"name":"Health Protection Agency, Centre for Infections, London, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Arnold","sequence":"additional","affiliation":[{"name":"Health Protection Agency, Centre for Infections, London, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan","family":"Bankier","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology, Medical Research Council, Cambridge, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul H.","family":"Dear","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Biology, Medical Research Council, Cambridge, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz","family":"Guerra","sequence":"additional","affiliation":[{"name":"Department of Biological Safety Molecular Diagnostic and Genetics, Federal Institute for Risk Assessment, Berlin, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katie L.","family":"Hopkins","sequence":"additional","affiliation":[{"name":"Health Protection Agency, Centre for Infections, London, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ernesto","family":"Liebana","sequence":"additional","affiliation":[{"name":"Department of Pathology, Veterinary Laboratories Agency, Weybridge, United Kingdom."},{"name":"Current address: European Food Safety Authority (EFSA), Parma, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dik J.","family":"Mevius","sequence":"additional","affiliation":[{"name":"Department of Bacteriology and TSEs, Central Institute for Animal Disease Control, Lelystad, The Netherlands."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. John","family":"Threlfall","sequence":"additional","affiliation":[{"name":"Health Protection Agency, Centre for Infections, London, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2008,6]]},"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2008.0812","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2008.0812","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:24:44Z","timestamp":1777667084000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/mdr.2008.0812"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,6]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2008,6]]}},"alternative-id":["10.1089\/mdr.2008.0812"],"URL":"https:\/\/doi.org\/10.1089\/mdr.2008.0812","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,6]]}}}