{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T23:21:28Z","timestamp":1790724088478,"version":"4.1.0"},"reference-count":0,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2008,9,1]],"date-time":"2008-09-01T00:00:00Z","timestamp":1220227200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2008,9]]},"abstract":"\n The aims of this study were to investigate the prevalence of\n qnrA<\/jats:italic>\n ,\n qnrB<\/jats:italic>\n , and\n qnrS<\/jats:italic>\n determinants and their molecular characteristics in ciprofloxacin-resistant isolates of\n Escherichia coli<\/jats:italic>\n and\n Klebsiella pneumoniae<\/jats:italic>\n from urinary tract infections (UTI) in Korea. A total of 202 nonduplicated clinical isolates of ciprofloxacin-resistant\n E. coli<\/jats:italic>\n (\n n<\/jats:italic>\n = 143) and\n K. pneumoniae<\/jats:italic>\n (\n n<\/jats:italic>\n = 59) were collected between July 2005 and August 2006. The\n qnr<\/jats:italic>\n determinant screening was carried out by PCR amplification of\n qnrA<\/jats:italic>\n ,\n qnrB<\/jats:italic>\n , and\n qnrS<\/jats:italic>\n , and all positive results were confirmed by direct sequencing of the PCR products. For\n qnr<\/jats:italic>\n -positive strains and their conjugants, antimicrobial susceptibility tests and pulsed-field gel electrophoresis (PFGE) were performed. The\n qnrB<\/jats:italic>\n gene was detected in 41 of the 202 isolates. Among 33 of 59 (55.9%)\n K. pneumoniae<\/jats:italic>\n isolates showing\n qnrB<\/jats:italic>\n , 29 isolates contained the\n qnrB4<\/jats:italic>\n gene, 3 isolates had the\n qnrB2<\/jats:italic>\n gene, and 1 isolate had the\n qnrB6<\/jats:italic>\n gene. All 8 (5.6%) of the\n qnrB<\/jats:italic>\n -positive isolates among the 143\n E. coli<\/jats:italic>\n strains possessed the\n qnrB4<\/jats:italic>\n gene. The minimum inhibitory concentrations (MICs) of ciprofloxacin for the transconjugants were 0.03\u20132 \u03bcg\/ml, representing an increase of 4- to 256-fold relative to the recipient,\n E. coli<\/jats:italic>\n J53Az\n r<\/jats:sup>\n . Resistances to various other antimicrobial agents also were transferred with the plasmid. The PFGE analysis revealed indistinguishable or closely related patterns in several strains and highly diverse patterns in general.\n QnrB<\/jats:italic>\n variants, especially the\n qnrB4<\/jats:italic>\n subtype, are highly prevalent in ciprofloxacin-resistant\n E. coli<\/jats:italic>\n and\n K. pneumoniae<\/jats:italic>\n from UTI in Korea. The emergence of plasmid-mediated quinolone resistance may contribute by several means to the rapid increase in bacterial resistance to these drugs.\n <\/jats:p>","DOI":"10.1089\/mdr.2008.0834","type":"journal-article","created":{"date-parts":[[2008,8,17]],"date-time":"2008-08-17T17:41:07Z","timestamp":1218994867000},"page":"221-226","source":"Crossref","is-referenced-by-count":36,"title":["High Rates of Plasmid-Mediated Quinolone Resistance\n QnrB<\/i>\n Variants Among Ciprofloxacin-Resistant\n Escherichia coli<\/i>\n and\n Klebsiella pneumoniae<\/i>\n from Urinary Tract Infections in Korea"],"prefix":"10.1177","volume":"14","author":[{"given":"Jeong Hwan","family":"Shin","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, College of Medicine, Inje University, Busan, Korea."},{"name":"Paik Institute for Clinical Research, Inje University, Busan, Korea."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hee Jung","family":"Jung","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, College of Medicine, Inje University, Busan, Korea."},{"name":"Paik Institute for Clinical Research, Inje University, Busan, Korea."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ja Young","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, College of Medicine, Inje University, Busan, Korea."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hye Ran","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, College of Medicine, Inje University, Busan, Korea."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeong Nyeo","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, College of Medicine, Inje University, Busan, Korea."},{"name":"Paik Institute for Clinical Research, Inje University, Busan, Korea."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chulhun Ludgerus","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, School of Medicine, Pusan National University, Busan, Korea."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2008,9]]},"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2008.0834","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2008.0834","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:24:49Z","timestamp":1777667089000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/mdr.2008.0834"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,9]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2008,9]]}},"alternative-id":["10.1089\/mdr.2008.0834"],"URL":"https:\/\/doi.org\/10.1089\/mdr.2008.0834","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,9]]}}}