{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T13:22:53Z","timestamp":1791638573987,"version":"4.3.4"},"reference-count":0,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2008,11,26]],"date-time":"2008-11-26T00:00:00Z","timestamp":1227657600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2008,12,1]]},"abstract":"\n The two multiple-antibiotic resistance (Mar) phenotypes (\n n<\/jats:italic>\n \u2009=\u20098, respectively) and susceptible isolates (\n n<\/jats:italic>\n \u2009=\u20094) of\n Shigella flexneri<\/jats:italic>\n from China were characterized involving the efflux pump AcrAB-TolC. The accumulation of ciprofloxacin,\n acrAB-tolC<\/jats:italic>\n PCR, and levels of mRNA with northern blots were performed in three groups. Sequencing of\n acrAB-tolC<\/jats:italic>\n was performed in selected isolates. An efflux inhibition was performed with Phe-Arg-\u03b2-naphthylamide. The accumulation of ciprofloxacin at steady state in susceptible isolates was significantly higher than that in the two Mar groups (\n p<\/jats:italic>\n \u2009<\u20090.05). The level of accumulation in the Mar strains was increased upon the addition of the protonophore carbonyl cyanidem-chlorophenylhydrazone. The expression level of\n acrA<\/jats:italic>\n mRNA in the Mar isolates was significantly higher than that in the susceptible isolates (\n p<\/jats:italic>\n \u2009<\u20090.05). Mar strain H26 had a single-nucleotide substitution in locus 322(G\u2192T) of\n acrA<\/jats:italic>\n , and Mar 0008 in locus 171(C\u2192A). The susceptible strain N15 had a base deletion in locus 36 (C) of\n tolC<\/jats:italic>\n gene. The role of the inhibitors of efflux pumps was significant in some isolates with the high expression of Mar pump genes. In conclusion, overexpression of\n acrA<\/jats:italic>\n gene leads to Mar in clinical isolates of\n S. flexneri<\/jats:italic>\n , and ciprofloxacin acquired susceptible to\n S. flexneri<\/jats:italic>\n with Phe-Arg-\u03b2-naphthylamide.\n <\/jats:p>","DOI":"10.1089\/mdr.2008.0847","type":"journal-article","created":{"date-parts":[[2008,11,26]],"date-time":"2008-11-26T14:40:00Z","timestamp":1227710400000},"page":"245-249","source":"Crossref","is-referenced-by-count":21,"title":["The AcrAB-TolC Pump Is Involved in Multidrug Resistance in Clinical\n Shigella flexneri<\/i>\n Isolates"],"prefix":"10.1177","volume":"14","author":[{"given":"Haiyan","family":"Yang","sequence":"first","affiliation":[{"name":"College of Public Health of Zhengzhou University","place":["People's Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangcai","family":"Duan","sequence":"additional","affiliation":[{"name":"College of Public Health of Zhengzhou University","place":["People's Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyuan","family":"Zhu","sequence":"additional","affiliation":[{"name":"College of Public Health of Zhengzhou University","place":["People's Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruili","family":"Lv","sequence":"additional","affiliation":[{"name":"College of Public Health of Zhengzhou University","place":["People's Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanlin","family":"Xi","sequence":"additional","affiliation":[{"name":"College of Public Health of Zhengzhou University","place":["People's Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weidong","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Public Health of Zhengzhou University","place":["People's Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingtang","family":"Fan","sequence":"additional","affiliation":[{"name":"Henan Key Laboratory of Molecular Medicine","place":["People's Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meixi","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Public Health of Zhengzhou University","place":["People's Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2008,11,26]]},"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2008.0847","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2008.0847","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:24:53Z","timestamp":1777667093000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/mdr.2008.0847"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,11,26]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2008,12,1]]}},"alternative-id":["10.1089\/mdr.2008.0847"],"URL":"https:\/\/doi.org\/10.1089\/mdr.2008.0847","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,11,26]]}}}