{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:23:01Z","timestamp":1777922581168,"version":"3.51.4"},"reference-count":0,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2009,12,1]],"date-time":"2009-12-01T00:00:00Z","timestamp":1259625600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2009,12]]},"abstract":"<jats:p>\n                    Extended-spectrum \u03b2-lactamases (ESBLs) in\n                    <jats:italic toggle=\"yes\">Pseudomonas aeruginosa<\/jats:italic>\n                    are increasingly reported worldwide. In our study, a total of 70 clinical isolates of multidrug-resistant\n                    <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                    were studied. Isoelectric focusing electrophoresis, PCR, and PCR product sequencing were designed to characterize the contained ESBLs. The Double Disk Synergy Test in Cloxacillin (250\u2009\u03bcg\/ml)\u2013containing Mueller\u2013Hinton agar plates with a 20\u2009mm distance between disks was the most reliable ESBL-screening method. Seven out of 70 multidrug-resistant\n                    <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                    clinical isolates were positive for ESBL and have the\n                    <jats:italic toggle=\"yes\">\n                      bla\n                      <jats:sub>SHV-2a<\/jats:sub>\n                    <\/jats:italic>\n                    ESBL gene. The\n                    <jats:italic toggle=\"yes\">\n                      bla\n                      <jats:sub>SHV-2a<\/jats:sub>\n                    <\/jats:italic>\n                    -positive isolates were clonally related according to Enterobacterial Repetetive Intergenic Consensus-PCR (ERIC-PCR) results. The\n                    <jats:italic toggle=\"yes\">\n                      bla\n                      <jats:sub>SHV-2a<\/jats:sub>\n                    <\/jats:italic>\n                    gene was found to be chromosomally located, and the flanking IS\n                    <jats:italic toggle=\"yes\">26<\/jats:italic>\n                    sequence in the immediate upstream region of the\n                    <jats:italic toggle=\"yes\">\n                      bla\n                      <jats:sub>SHV-2a<\/jats:sub>\n                    <\/jats:italic>\n                    gene was detected in all SHV-2a\u2013producing isolates. This is the first report of SHV-2a\u2013producing\n                    <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                    isolates from Tunisia.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2009.0012","type":"journal-article","created":{"date-parts":[[2009,10,26]],"date-time":"2009-10-26T13:06:58Z","timestamp":1256562418000},"page":"295-301","source":"Crossref","is-referenced-by-count":16,"title":["Emergence of SHV-2a Extended-Spectrum \u03b2-Lactamases in Clinical Isolates of\n                    <i>Pseudomonas aeruginosa<\/i>\n                    in a University Hospital in Tunisia"],"prefix":"10.1177","volume":"15","author":[{"given":"Wejdene","family":"Mansour","sequence":"first","affiliation":[{"name":"Laboratory of Microbiology","place":["Tunisia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Safia","family":"Dahmen","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology","place":["Tunisia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laurent","family":"Poirel","sequence":"additional","affiliation":[{"name":"National Institute of Health and Medical Research (INSERM), and University of Paris","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karama","family":"Charfi","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology","place":["Tunisia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dalia","family":"Bettaieb","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology","place":["Tunisia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noureddine","family":"Boujaafar","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology","place":["Tunisia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olfa","family":"Bouallegue","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology","place":["Tunisia"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2009,12]]},"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2009.0012","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2009.0012","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:24:56Z","timestamp":1777667096000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/mdr.2009.0012"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,12]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2009,12]]}},"alternative-id":["10.1089\/mdr.2009.0012"],"URL":"https:\/\/doi.org\/10.1089\/mdr.2009.0012","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,12]]}}}