{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:44:07Z","timestamp":1791938647044,"version":"4.3.4"},"reference-count":46,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2010,3,1]],"date-time":"2010-03-01T00:00:00Z","timestamp":1267401600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2010,3]]},"abstract":"<jats:p>\n                    To understand the mechanisms and epidemiology of antimicrobial resistance (AR), the genetic elements responsible must be identified. Due to the myriad of possible genes, a high-density genotyping technique is needed for initial screening. To achieve this, AR genes in the National Center for Biotechnology Information GenBank database were identified by their annotations and compiled into a nonredundant list of 775 genes. A DNA microarray was constructed of 70mer oligonucelotide probes designed to detect these genes encoding resistances to aminoglycosides, \u03b2-lactams, chloramphenicols, glycopeptides, heavy metals, lincosamides, macrolides, metronidazoles, polyketides, quaternary ammonium compounds, streptogramins, sulfonamides, tetracyclines, and trimethoprims as well as resistance transfer genes. The microarray was validated with two fully sequenced control strains of\n                    <jats:italic toggle=\"yes\">Salmonella enterica<\/jats:italic>\n                    : Typhimurium LT2 (sensitive) and Typhi CT18 (multidrug resistance [MDR]). All resistance genes encoded on the MDR plasmid, pHCM1, harbored by CT18 were detected in that strain, whereas no resistance genes were detected in LT2. The microarray was also tested with a variety of bacteria, including MDR\n                    <jats:italic toggle=\"yes\">Salmonella enterica<\/jats:italic>\n                    serovars,\n                    <jats:italic toggle=\"yes\">Escherichia coli, Campylobacter<\/jats:italic>\n                    spp.,\n                    <jats:italic toggle=\"yes\">Enterococcus<\/jats:italic>\n                    spp., methicillin-resistant\n                    <jats:italic toggle=\"yes\">Staphylococcus aureus, Listeria<\/jats:italic>\n                    spp., and\n                    <jats:italic toggle=\"yes\">Clostridium difficile<\/jats:italic>\n                    . The results presented here demonstrate that a microarray can be designed to detect virtually all AR genes found in the National Center for Biotechnology Information database, thus reducing the subsequent assays necessary to identify specific resistance gene alleles.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2009.0082","type":"journal-article","created":{"date-parts":[[2009,11,16]],"date-time":"2009-11-16T13:10:09Z","timestamp":1258377009000},"page":"9-19","source":"Crossref","is-referenced-by-count":57,"title":["Development of a DNA Microarray to Detect Antimicrobial Resistance Genes Identified in the National Center for Biotechnology Information Database"],"prefix":"10.1177","volume":"16","author":[{"given":"Jonathan G.","family":"Frye","sequence":"first","affiliation":[{"name":"Agriculture Research Service","place":["Georgia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca L.","family":"Lindsey","sequence":"additional","affiliation":[{"name":"Agriculture Research Service","place":["Georgia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaelle","family":"Rondeau","sequence":"additional","affiliation":[{"name":"Sidney Kimmel Cancer Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steffen","family":"Porwollik","sequence":"additional","affiliation":[{"name":"Sidney Kimmel Cancer Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fred","family":"Long","sequence":"additional","affiliation":[{"name":"Sidney Kimmel Cancer Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"McClelland","sequence":"additional","affiliation":[{"name":"Sidney Kimmel Cancer Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlene R.","family":"Jackson","sequence":"additional","affiliation":[{"name":"Agriculture Research Service","place":["Georgia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark D.","family":"Englen","sequence":"additional","affiliation":[{"name":"Agriculture Research Service","place":["Georgia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard J.","family":"Meinersmann","sequence":"additional","affiliation":[{"name":"Agriculture Research Service","place":["Georgia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark E.","family":"Berrang","sequence":"additional","affiliation":[{"name":"Agriculture Research Service","place":["Georgia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johnnie A.","family":"Davis","sequence":"additional","affiliation":[{"name":"Agriculture Research Service","place":["Georgia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John B.","family":"Barrett","sequence":"additional","affiliation":[{"name":"Agriculture Research Service","place":["Georgia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer B.","family":"Turpin","sequence":"additional","affiliation":[{"name":"Agriculture Research Service","place":["Georgia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sutawee N.","family":"Thitaram","sequence":"additional","affiliation":[{"name":"Agriculture Research Service","place":["Georgia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula J.","family":"Fedorka-Cray","sequence":"additional","affiliation":[{"name":"Agriculture Research Service","place":["Georgia"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2010,3]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.arcmed.2005.06.009"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2007.11.017"},{"key":"e_1_3_3_4_2","doi-asserted-by":"crossref","first-page":"78","DOI":"10.2144\/00291st01","article-title":"Analysis of DNA microarrays by non-destructive fluorescent staining using SYBR green II","volume":"29","author":"Battaglia C.","year":"2000","unstructured":"Battaglia C., , Salani G., , Consolandi C., , Bernardi L.R., , De Bellis G. 2000. Analysis of DNA microarrays by non-destructive fluorescent staining using SYBR green II. Biotechniques, 29:78\u201381.","journal-title":"Biotechniques"},{"key":"e_1_3_3_5_2","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1016\/S1369-5274(02)00362-4","article-title":"Toward rapid real-time molecular diagnostic to guide smart use of antimicrobials","volume":"5","author":"Boissinot M.","year":"2002","unstructured":"Boissinot M., , Bergeron M.G. 2002. Toward rapid real-time molecular diagnostic to guide smart use of antimicrobials. Curr. Opin. Microbiol., 5:478\u2013482.","journal-title":"Curr. Opin. Microbiol."},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.72.5.3780-3784.2006"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00450-08"},{"key":"e_1_3_3_8_2","doi-asserted-by":"crossref","first-page":"3290","DOI":"10.1128\/AAC.47.10.3290-3295.2003","article-title":"Identifying antimicrobial resistance genes with DNA microarrays","volume":"47","author":"Call D.R.","year":"2003","unstructured":"Call D.R., , Bakko M.K., , Krug M.J., , Roberts M.C. 2003. Identifying antimicrobial resistance genes with DNA microarrays. Antimicrob. Agents Chemother., 47:3290\u20133295.","journal-title":"Antimicrob. Agents Chemother."},{"key":"e_1_3_3_9_2","doi-asserted-by":"crossref","first-page":"2038","DOI":"10.1128\/AAC.01574-05","article-title":"DNA microarray for detection of macrolide resistance genes","volume":"50","author":"Cassone M.","year":"2006","unstructured":"Cassone M., , D'Andrea M.M., , Iannelli F., , Oggioni M.R., , Rossolini G.M., , Pozzi G. 2006. DNA microarray for detection of macrolide resistance genes. Antimicrob. Agents Chemother., 50:2038\u20132041.","journal-title":"Antimicrob. Agents Chemother."},{"key":"e_1_3_3_10_2","doi-asserted-by":"crossref","first-page":"2658","DOI":"10.2741\/2262","article-title":"Bacterial DNA microarrays for clinical microbiology: the early logarithmic phase","volume":"12","author":"Cassone M.","year":"2007","unstructured":"Cassone M., , Giordano A., , Pozzi G. 2007. Bacterial DNA microarrays for clinical microbiology: the early logarithmic phase. Front Biosci., 12:2658\u20132669.","journal-title":"Front Biosci."},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcp.2004.11.008"},{"key":"e_1_3_3_12_2","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1186\/1471-2164-9-497","article-title":"Experimental analysis of oligonucleotide microarray design criteria to detect deletions by comparative genomic hybridization","volume":"9","author":"Flibotte S.","year":"2008","unstructured":"Flibotte S., , Moerman D.G. 2008. Experimental analysis of oligonucleotide microarray design criteria to detect deletions by comparative genomic hybridization. BMC Genomics, 9:497\u2013508.","journal-title":"BMC Genomics"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.4.836-871.2001"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2005.09.021"},{"key":"e_1_3_3_15_2","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1186\/1471-2180-8-195","article-title":"A short-oligonucleotide microarray that allows improved detection of gastrointestinal tract microbial communities","volume":"8","author":"Harrington C.R.","year":"2008","unstructured":"Harrington C.R., , Lucchini S., , Ridgway K.P., , Wegmann U., , Eaton T.J., , Hinton J.C., , Gasson M.J., , Narbad A. 2008. A short-oligonucleotide microarray that allows improved detection of gastrointestinal tract microbial communities. BMC Microbiol., 8:195\u2013214.","journal-title":"BMC Microbiol."},{"key":"e_1_3_3_16_2","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1016\/j.mib.2005.08.001","article-title":"Point-of-care molecular diagnostic systems\u2014past, present and future","volume":"8","author":"Holland C.A.","year":"2005","unstructured":"Holland C.A., , Kiechle F.L. 2005. Point-of-care molecular diagnostic systems\u2014past, present and future. Curr. Opin. Microbiol., 8:504\u2013509.","journal-title":"Curr. Opin. Microbiol."},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2007.779"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-005-0279-0"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.229202"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(06)10004-X"},{"key":"e_1_3_3_21_2","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1111\/j.1574-6968.2000.tb09153.x","article-title":"Prevalence of the multiple antibiotic resistance operon (marRAB) in the genus Salmonella","volume":"187","author":"Kunonga N.I.","year":"2000","unstructured":"Kunonga N.I., , Sobieski R.J., , Crupper S.S. 2000. Prevalence of the multiple antibiotic resistance operon (marRAB) in the genus Salmonella. FEMS Microbiol. Lett., 187:155\u2013160.","journal-title":"FEMS Microbiol. Lett."},{"key":"e_1_3_3_22_2","doi-asserted-by":"crossref","first-page":"228","DOI":"10.2527\/1995.731228x","article-title":"High-lysine corn as a source of protein and energy for finishing calves","volume":"73","author":"Ladely S.R.","year":"1995","unstructured":"Ladely S.R., , Stock R.A., , Klopfenstein T.J., , Sindt M.H. 1995. High-lysine corn as a source of protein and energy for finishing calves. J. Anim. Sci., 73:228\u2013235.","journal-title":"J. Anim. Sci."},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1177\/153537020523000810"},{"key":"e_1_3_3_24_2","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1093\/bfgp\/2.1.21","article-title":"Advances in spotted microarray resources for expression profiling","volume":"2","author":"Lyons P.","year":"2003","unstructured":"Lyons P. 2003. Advances in spotted microarray resources for expression profiling. Brief. Funct. Genomic. Proteomic., 2:21\u201330.","journal-title":"Brief. Funct. Genomic. Proteomic."},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1177\/104063870701900204"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm326"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcp.2006.08.005"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki253"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/35101614"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-71.4.760"},{"key":"e_1_3_3_31_2","doi-asserted-by":"crossref","first-page":"1048","DOI":"10.1128\/JCM.42.3.1048-1057.2004","article-title":"Microarray-based identification of bacteria in clinical samples by solid-phase PCR amplification of 23S ribosomal DNA sequences","volume":"42","author":"Mitterer G.","year":"2004","unstructured":"Mitterer G., , Huber M., , Leidinger E., , Kirisits C., , Lubitz W., , Mueller M.W., , Schmidt W.M. 2004. Microarray-based identification of bacteria in clinical samples by solid-phase PCR amplification of 23S ribosomal DNA sequences. J. Clin. Microbiol., 42:1048\u20131057.","journal-title":"J. Clin. Microbiol."},{"key":"e_1_3_3_32_2","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1111\/j.1469-0691.2008.01986.x","article-title":"DNA microarray-based genotyping of methicillin-resistant Staphylococcus aureus strains from Eastern Saxony","volume":"14","author":"Monecke S.","year":"2008","unstructured":"Monecke S., , Jatzwauk L., , Weber S., , Slickers P., , Ehricht R. 2008. DNA microarray-based genotyping of methicillin-resistant Staphylococcus aureus strains from Eastern Saxony. Clin. Microbiol. Infect, 14:534\u2013545.","journal-title":"Clin. Microbiol. Infect"},{"key":"e_1_3_3_33_2","volume-title":"Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals","author":"[NCCLS] National Committee for Clinical Laboratory Standards","year":"2002","unstructured":"[NCCLS] National Committee for Clinical Laboratory Standards. 2002. Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals. Wayne, PA."},{"key":"e_1_3_3_34_2","volume-title":"Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically","author":"[NCCLS] National Committee for Clinical Laboratory Standards","year":"2003","unstructured":"[NCCLS] National Committee for Clinical Laboratory Standards. 2003. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Wayne, PA."},{"key":"e_1_3_3_35_2","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1128\/iai.33.2.555-564.1981","article-title":"Ability of Neisseria gonorrhoeae, Neisseria meningitidis, and commensal Neisseria species to obtain iron from transferrin and iron compounds","volume":"33","author":"Nickelsen P.A.","year":"1981","unstructured":"Nickelsen P.A., , Sparling P.F. 1981. Ability of Neisseria gonorrhoeae, Neisseria meningitidis, and commensal Neisseria species to obtain iron from transferrin and iron compounds. Infect Immun, 33:555\u2013564.","journal-title":"Infect Immun"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg622"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/35101607"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.5.2291-2302.2005"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg298"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0027-5107(01)00239-1"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-8-63"},{"key":"e_1_3_3_42_2","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1046\/j.1365-2672.2003.02046.x","article-title":"Microarray analysis of erythromycin resistance determinants","volume":"95","author":"Volokhov D.","year":"2003","unstructured":"Volokhov D., , Chizhikov V., , Chumakov K., , Rasooly A. 2003. Microarray analysis of erythromycin resistance determinants. J. Appl. Microbiol., 95:787\u2013798.","journal-title":"J. Appl. Microbiol."},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0505033102"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gki399"},{"key":"e_1_3_3_45_2","doi-asserted-by":"crossref","first-page":"4083","DOI":"10.1128\/JCM.42.9.4083-4091.2004","article-title":"Development and validation of a diagnostic DNA microarray to detect quinolone-resistant Escherichia coli among clinical isolates","volume":"42","author":"Yu X.","year":"2004","unstructured":"Yu X., , Susa M., , Knabbe C., , Schmid R.D., , Bachmann T.T. 2004. Development and validation of a diagnostic DNA microarray to detect quinolone-resistant Escherichia coli among clinical isolates. J. Clin. Microbiol., 42:4083\u20134091.","journal-title":"J. Clin. Microbiol."},{"key":"e_1_3_3_46_2","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/j.ijmm.2007.03.018","article-title":"Rapid and sensitive detection of fluoroquinolone-resistant Escherichia coli from urine samples using a genotyping DNA microarray","volume":"297","author":"Yu X.","year":"2007","unstructured":"Yu X., , Susa M., , Weile J., , Knabbe C., , Schmid R.D., , Bachmann T.T. 2007. Rapid and sensitive detection of fluoroquinolone-resistant Escherichia coli from urine samples using a genotyping DNA microarray. Int. J. Med. Microbiol., 297:417\u2013429.","journal-title":"Int. J. Med. Microbiol."},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2009.04270.x"}],"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/mdr.2009.0082","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2009.0082","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2009.0082","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:25:04Z","timestamp":1777667104000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/mdr.2009.0082"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,3]]},"references-count":46,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2010,3]]}},"alternative-id":["10.1089\/mdr.2009.0082"],"URL":"https:\/\/doi.org\/10.1089\/mdr.2009.0082","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,3]]}}}