{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T07:44:24Z","timestamp":1789803864260,"version":"4.0.1"},"reference-count":0,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2009,9,1]],"date-time":"2009-09-01T00:00:00Z","timestamp":1251763200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2009,9]]},"abstract":"\n One hundred twelve fecal samples of healthy children were recovered in Portugal during October 2007 and February 2008 and were tested for extended-spectrum \u03b2-lactamases (ESBLs) containing\n Escherichia coli<\/jats:italic>\n isolates and vancomycin-resistant enterococci (VRE). Three of the 112 fecal samples (2.7%) harbored ESBL-positive\n E. coli<\/jats:italic>\n isolates and the\n bla<\/jats:italic>\n CTX-M-1<\/jats:sub>\n ,\n bla<\/jats:italic>\n TEM-52<\/jats:sub>\n , and\n bla<\/jats:italic>\n SHV-12<\/jats:sub>\n genes were identified in these isolates. The\n bla<\/jats:italic>\n TEM-52<\/jats:sub>\n -containing isolate showed a phenotype of multiresistance that included fluoroquinolones, tetracycline, trimethoprim\u2013sulfamethoxazole, and chloramphenicol;\n sul<\/jats:italic>\n 1,\n sul<\/jats:italic>\n 3, and\n cml<\/jats:italic>\n A genes were detected in this isolate, in addition to two amino acid changes in GyrA (Ser83Leu\u2009+\u2009Asp87Asn) and one in ParC protein (Ser80Ile). The ESBL isolates corresponded to phylogroup A (one isolate), B1 (one isolate), and D (one isolate).\n van<\/jats:italic>\n A-containing\n Escherichia faecium<\/jats:italic>\n isolates were detected in 13 of the 112 fecal samples (11.6%), and\n van<\/jats:italic>\n C-1 isolates were found in 2 samples. A diversity of resistance genes [(\n tet<\/jats:italic>\n (M),\n tet<\/jats:italic>\n (L),\n erm<\/jats:italic>\n (B),\n aph<\/jats:italic>\n (3\u2032)-IIIa,\n ant<\/jats:italic>\n (6)-Ia,\n cat<\/jats:italic>\n A, and\n vat<\/jats:italic>\n (E)] were found in VRE isolates. These results show that the intestinal tract of healthy children constitutes a reservoir of ESBL-containing\n E. coli<\/jats:italic>\n and VRE isolates.\n <\/jats:p>","DOI":"10.1089\/mdr.2009.0910","type":"journal-article","created":{"date-parts":[[2009,9,3]],"date-time":"2009-09-03T09:37:46Z","timestamp":1251970666000},"page":"211-216","source":"Crossref","is-referenced-by-count":38,"title":["Genetic Detection of Extended-Spectrum \u03b2-Lactamase\u2013Containing\n Escherichia coli<\/i>\n Isolates and Vancomycin-Resistant Enterococci in Fecal Samples of Healthy Children"],"prefix":"10.1177","volume":"15","author":[{"given":"Bruno","family":"Guimar\u00e3es","sequence":"first","affiliation":[{"name":"University of Tr\u00e1s-os-Montes and Alto Douro","place":["Portugal"]},{"name":"Center of Studies of Animal and Veterinary Sciences","place":["Portugal"]},{"name":"University of Tr\u00e1s-os-Montes and Alto Douro","place":["Portugal"]},{"name":"University of Tr\u00e1s-os-Montes and Alto Douro","place":["Portugal"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c2ngela","family":"Barreto","sequence":"additional","affiliation":[{"name":"University of Tr\u00e1s-os-Montes and Alto Douro","place":["Portugal"]},{"name":"Center of Studies of Animal and Veterinary Sciences","place":["Portugal"]},{"name":"University of Tr\u00e1s-os-Montes and Alto Douro","place":["Portugal"]},{"name":"University of Tr\u00e1s-os-Montes and Alto Douro","place":["Portugal"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hajer","family":"Radhouani","sequence":"additional","affiliation":[{"name":"University of Tr\u00e1s-os-Montes and Alto Douro","place":["Portugal"]},{"name":"Center of Studies of Animal and Veterinary Sciences","place":["Portugal"]},{"name":"University of Tr\u00e1s-os-Montes and Alto Douro","place":["Portugal"]},{"name":"University of Tr\u00e1s-os-Montes and Alto Douro","place":["Portugal"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Figueiredo","sequence":"additional","affiliation":[{"name":"University of Tr\u00e1s-os-Montes and Alto Douro","place":["Portugal"]},{"name":"Center of Studies of Animal and Veterinary Sciences","place":["Portugal"]},{"name":"University of Tr\u00e1s-os-Montes and Alto Douro","place":["Portugal"]},{"name":"University of Tr\u00e1s-os-Montes and Alto Douro","place":["Portugal"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eurico","family":"Gaspar","sequence":"additional","affiliation":[{"name":"Hospital of Tr\u00e1s-os-Montes and Alto Douro","place":["Portugal"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"University of Tr\u00e1s-os-Montes and Alto Douro","place":["Portugal"]},{"name":"Center of Studies of Animal and Veterinary Sciences","place":["Portugal"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Torres","sequence":"additional","affiliation":[{"name":"University of La Rioja","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilberto","family":"Igrejas","sequence":"additional","affiliation":[{"name":"University of Tr\u00e1s-os-Montes and Alto Douro","place":["Portugal"]},{"name":"University of Tr\u00e1s-os-Montes and Alto Douro","place":["Portugal"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patr\u00edcia","family":"Poeta","sequence":"additional","affiliation":[{"name":"University of Tr\u00e1s-os-Montes and Alto Douro","place":["Portugal"]},{"name":"Center of Studies of Animal and Veterinary Sciences","place":["Portugal"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2009,9]]},"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2009.0910","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2009.0910","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:25:30Z","timestamp":1777667130000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/mdr.2009.0910"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,9]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2009,9]]}},"alternative-id":["10.1089\/mdr.2009.0910"],"URL":"https:\/\/doi.org\/10.1089\/mdr.2009.0910","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,9]]}}}