{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T03:31:22Z","timestamp":1784863882909,"version":"3.55.0"},"reference-count":16,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2012,8,1]],"date-time":"2012-08-01T00:00:00Z","timestamp":1343779200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2012,8]]},"abstract":"<jats:p>\n                    <jats:bold>\n                      <jats:italic toggle=\"yes\">Aims:<\/jats:italic>\n                    <\/jats:bold>\n                    This study sought to analyze the molecular mechanisms contributing to the development of rifaximin (Rfx) resistance\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    in\n                    <jats:italic toggle=\"yes\">Escherichia coli.<\/jats:italic>\n                    Twenty-eight Rfx-resistant mutants as well as four clinical isolates of\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    were analyzed. The results obtained show that mutations in the\n                    <jats:italic toggle=\"yes\">rpoB<\/jats:italic>\n                    gene and overexpression of Phe-Arg-\u03b2-naphthylamide (PA\u03b2N)-inhibitible efflux pump were implicated in Rfx resistance.\n                    <jats:bold>\n                      <jats:italic toggle=\"yes\">Results:<\/jats:italic>\n                    <\/jats:bold>\n                    Amino acid substitutions at position 516 of the \u03b2-subunit of RNA polymerases were the most frequently obtained (53.6% of the mutants). The efflux pump inhibitor decreased the minimal inhibitory concentration (MIC) of 71.43% (20\/28) of the mutant strains.\n                    <jats:bold>\n                      <jats:italic toggle=\"yes\">Conclusions:<\/jats:italic>\n                    <\/jats:bold>\n                    Mutations studied in the\n                    <jats:italic toggle=\"yes\">rpoB<\/jats:italic>\n                    gene and overexpression of PA\u03b2N-inhibitible efflux pumps contribute to Rfx resistance (together or not), whereas alterations in porin levels do not seem to have a relevant role in the acquisition of Rfx resistance.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2011.0010","type":"journal-article","created":{"date-parts":[[2011,6,28]],"date-time":"2011-06-28T15:44:43Z","timestamp":1309275883000},"page":"376-379","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":17,"title":["Fitness and Molecular Mechanisms of Resistance to Rifaximin in\n                    <i>In Vitro<\/i>\n                    Selected\n                    <i>Escherichia coli<\/i>\n                    Mutants"],"prefix":"10.1177","volume":"18","author":[{"given":"Maria J.","family":"Pons","sequence":"first","affiliation":[{"name":"IDIBAPS\u2014Hospital Clinic-Universitat de Barcelona","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Mensa","sequence":"additional","affiliation":[{"name":"IDIBAPS\u2014Hospital Clinic-Universitat de Barcelona","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joaquim","family":"Gasc\u00f3n","sequence":"additional","affiliation":[{"name":"IDIBAPS\u2014Hospital Clinic-Universitat de Barcelona","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joaquim","family":"Ruiz","sequence":"additional","affiliation":[{"name":"IDIBAPS\u2014Hospital Clinic-Universitat de Barcelona","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2012,8]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"crossref","first-page":"4886","DOI":"10.1073\/pnas.0711939105","article-title":"Rifamycin antibiotic resistance by ADP-ribosylation structure and diversity of Arr","volume":"105","author":"Baysarowich J.","year":"2008","unstructured":"Baysarowich J., , Koteva K., , Hughes D., , Ejim L., , Griffiths E., , Zhang K., , Junop M., , Wright G.D. 2008. Rifamycin antibiotic resistance by ADP-ribosylation structure and diversity of Arr. Proc. Natl. Acad. Sci., 105:4886\u20134891.","journal-title":"Proc. Natl. Acad. Sci."},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)71877-8"},{"issue":"1","key":"e_1_3_3_4_2","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1159\/000089785","article-title":"Epidemiology, etiology and pathophysiology of traveler's diarrhea","volume":"73","author":"Gasc\u00f3n J.","year":"2006","unstructured":"Gasc\u00f3n J. 2006. Epidemiology, etiology and pathophysiology of traveler's diarrhea. Digestion, 73,Suppl 1:102\u2013108.","journal-title":"Digestion"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.317.7159.647"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2004.05.019"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2036.2010.04296.x"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2007.01903.x"},{"key":"e_1_3_3_9_2","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1111\/j.1600-0463.2007.apm_567.x","article-title":"Trends in antimicrobial resistance in Campylobacter spp. causing traveller's diarrhea","volume":"115","author":"Ruiz J.","year":"2007","unstructured":"Ruiz J., , Marco F., , Oliveira I., , Vila J., , Gasc\u00f3n J. 2007. Trends in antimicrobial resistance in Campylobacter spp. causing traveller's diarrhea. APMIS, 115:218\u2013224.","journal-title":"APMIS"},{"key":"e_1_3_3_10_2","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1016\/j.diagmicrobio.2007.07.003","article-title":"In vitro antimicrobial activity of rifaximin against enteropathogens causing traveller's diarrhea","volume":"59","author":"Ruiz J.","year":"2007","unstructured":"Ruiz J., , Mensa L., , O'Callaghan C., , Pons M.J., , Gonzalez A., , Vila J., , Gasc\u00f3n J. 2007. In vitro antimicrobial activity of rifaximin against enteropathogens causing traveller's diarrhea. Diagn. Microbiol. Infect. Dis., 59:473\u2013475.","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"e_1_3_3_11_2","doi-asserted-by":"crossref","first-page":"1016","DOI":"10.1093\/jac\/dkn078","article-title":"Development of Escherichia coli rifaximin-resistant mutants: frequency of selection and stability","volume":"61","author":"Ruiz J.","year":"2008","unstructured":"Ruiz J., , Mensa L., , Pons M.J., , Vila J., , Gasc\u00f3n J. 2008. Development of Escherichia coli rifaximin-resistant mutants: frequency of selection and stability. J. Antimicrob. Chemother., 61:1016\u20131019.","journal-title":"J. Antimicrob. Chemother."},{"key":"e_1_3_3_12_2","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1093\/jac\/dkh117","article-title":"Effect of efflux pump inhibitor Phe-Arg-\u03b2-naphthylamide on the MIC values of the quinolones, tetracycline and chloramphenicol, in Escherichia coli isolates of different origin","volume":"53","author":"S\u00e1enz Y.","year":"2002","unstructured":"S\u00e1enz Y., , Ruiz J., , Zarazaga M., , Teixid\u00f3 M., , Torres C., , Vila J. 2002. Effect of efflux pump inhibitor Phe-Arg-\u03b2-naphthylamide on the MIC values of the quinolones, tetracycline and chloramphenicol, in Escherichia coli isolates of different origin. J. Antimicrob. Chemother., 53:544\u2013545.","journal-title":"J. Antimicrob. Chemother."},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1159\/000081990"},{"key":"e_1_3_3_14_2","doi-asserted-by":"crossref","first-page":"14820","DOI":"10.1016\/S0021-9258(18)82407-3","article-title":"New rifampicin-resistant and streptolydigin\u2013resistant mutants in the \u03b2 subunit of Escherichia coli RNA polymerase","volume":"268","author":"Severinov K.","year":"1993","unstructured":"Severinov K., , Soushko M., , Goldfarbs A., , Nikiforov V. 1993. New rifampicin-resistant and streptolydigin\u2013resistant mutants in the \u03b2 subunit of Escherichia coli RNA polymerase. J. Biol. Chem., 268:14820\u201314825.","journal-title":"J. Biol. Chem."},{"key":"e_1_3_3_15_2","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1099\/jmm.0.45613-0","article-title":"Enhanced active efflux, repression of porin synthesis and development of Mar phenotype by diazepam in two enterobacteria strains","volume":"53","author":"Tav\u00edo M.","year":"2004","unstructured":"Tav\u00edo M., , Vila J., , Perilli M., , Casa\u00f1as L., , Maci\u00e1 L., , Amicosante G., , Jim\u00e9nez de Anta M.T. 2004. Enhanced active efflux, repression of porin synthesis and development of Mar phenotype by diazepam in two enterobacteria strains. J. Med. Microb., 53:1119\u20131122.","journal-title":"J. Med. Microb."},{"key":"e_1_3_3_16_2","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1016\/j.ijantimicag.2009.12.017","article-title":"Resistance to rifampicin: at the crossroads between ecological, genomic and medical concerns","volume":"35","author":"Tupin A.","year":"2010","unstructured":"Tupin A., , Gualtieri M., , Roquet-Ban\u00e8res F., , Morichaud Z., , Brodolin K., , Leonetti J.P. 2010. Resistance to rifampicin: at the crossroads between ecological, genomic and medical concerns. Int. J. Antimicrob. Agents, 35:519\u2013523.","journal-title":"Int. J. Antimicrob. Agents"},{"key":"e_1_3_3_17_2","doi-asserted-by":"crossref","first-page":"2783","DOI":"10.1128\/JB.187.8.2783-2792.2005","article-title":"Cross-resistance of Escherichia coli RNA polymerases confering rifampin resistance to different antibiotics","volume":"187","author":"Xu M.","year":"2005","unstructured":"Xu M., , Ning Y., , Goldstein B.P., , Jin D.J. 2005. Cross-resistance of Escherichia coli RNA polymerases confering rifampin resistance to different antibiotics. J. Bacteriol., 187:2783\u20132792.","journal-title":"J. Bacteriol."}],"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/mdr.2011.0010","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2011.0010","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2011.0010","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:26:17Z","timestamp":1777667177000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/mdr.2011.0010"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,8]]},"references-count":16,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2012,8]]}},"alternative-id":["10.1089\/mdr.2011.0010"],"URL":"https:\/\/doi.org\/10.1089\/mdr.2011.0010","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,8]]}}}