{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:23:56Z","timestamp":1777922636115,"version":"3.51.4"},"reference-count":19,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2011,12,1]],"date-time":"2011-12-01T00:00:00Z","timestamp":1322697600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:p>Extended-spectrum \u03b2-lactamases (ESBLs) belonging to the TEM and SHV families were investigated in 583 ESBL-producing Enterobacteriaceae collected at the clinical microbiology laboratories of 11 teaching Italian hospitals. By molecular analysis TEM-type and SHV-type ESBLs were confirmed on 154 and 74 isolates, respectively. High variability was found among TEM-types \u03b2-lactamases with the following variants: TEM-5, TEM-6, TEM-12, TEM-15, TEM-24, TEM-26, TEM-29, TEM-52, TEM-92, TEM-134, and TEM-149. Among SHV variants, SHV-2a, SHV-5, SHV-12, and SHV-28 have been detected. The most widespread variants are TEM-52\/92 and SHV-12.<\/jats:p>","DOI":"10.1089\/mdr.2011.0059","type":"journal-article","created":{"date-parts":[[2011,10,4]],"date-time":"2011-10-04T12:32:17Z","timestamp":1317731537000},"page":"521-524","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":15,"title":["Persistence of TEM-52\/TEM-92 and SHV-12 Extended-Spectrum \u03b2-Lactamases in Clinical Isolates of Enterobacteriaceae in Italy"],"prefix":"10.1177","volume":"17","author":[{"given":"Mariagrazia","family":"Perilli","sequence":"first","affiliation":[{"name":"University of L'Aquila","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernardetta","family":"Segatore","sequence":"additional","affiliation":[{"name":"University of L'Aquila","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Mugnaioli","sequence":"additional","affiliation":[{"name":"University of Siena","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuseppe","family":"Celenza","sequence":"additional","affiliation":[{"name":"University of L'Aquila","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gian Maria","family":"Rossolini","sequence":"additional","affiliation":[{"name":"University of Siena","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefania","family":"Stefani","sequence":"additional","affiliation":[{"name":"University of Catania","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesco","family":"Luzzaro","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatrice","family":"Pini","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gianfranco","family":"Amicosante","sequence":"additional","affiliation":[{"name":"University of L'Aquila","place":["Italy"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2011,12]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.4.933-951.2001"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkl483"},{"key":"e_1_3_3_4_2","doi-asserted-by":"crossref","first-page":"1659","DOI":"10.1128\/JCM.44.5.1659-1664.2006","article-title":"Trends in production of extended-spectrum \u03b2-lactamases among enterobacteria of medical interest: report of the second Italian nationwide survey","volume":"44","author":"Luzzaro F.","year":"2006","unstructured":"Luzzaro F., , Mezzatesta M., , Mugnaioli C., , Perilli M., , Stefani S., , Amicosante G., , Rossolini G.M., , Toniolo A. 2006. Trends in production of extended-spectrum \u03b2-lactamases among enterobacteria of medical interest: report of the second Italian nationwide survey. J. Clin. Microbiol., 44:1659\u20131664.","journal-title":"J. Clin. Microbiol."},{"key":"e_1_3_3_5_2","doi-asserted-by":"crossref","first-page":"3220","DOI":"10.1128\/AAC.00473-06","article-title":"Dissemination in Portugal of CTX-M-15-, OXA-1-, and TEM-1-producing Enterobacteriaceae strains containing the aac(6\u2019)-Ib-cr gene, which encodes an aminoglycoside- and fluoroquinolone-modifying enzyme","volume":"50","author":"Machado E.","year":"2006","unstructured":"Machado E. 2006. Dissemination in Portugal of CTX-M-15-, OXA-1-, and TEM-1-producing Enterobacteriaceae strains containing the aac(6\u2019)-Ib-cr gene, which encodes an aminoglycoside- and fluoroquinolone-modifying enzyme. Antimicrob. Agents Chemother., 50:3220\u20133221.","journal-title":"Antimicrob. Agents Chemother."},{"key":"e_1_3_3_6_2","doi-asserted-by":"crossref","first-page":"2700","DOI":"10.1128\/AAC.00068-06","article-title":"CTX-M-type extended-spectrum \u00df-lactamases in Italy: molecular epidemiology of an emerging countrywide problem","volume":"50","author":"Mugnaioli C.","year":"2006","unstructured":"Mugnaioli C., , Luzzaro F., , De Luca F., , Brigante G., , Perilli M., , Amicosante G., , Stefani S., , Toniolo A., , Rossolini G.M. 2006. CTX-M-type extended-spectrum \u00df-lactamases in Italy: molecular epidemiology of an emerging countrywide problem. Antimicrob. Agents Chemother., 50:2700\u20132706.","journal-title":"Antimicrob. Agents Chemother."},{"key":"e_1_3_3_7_2","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1038\/84981","article-title":"Predicting the emergence of antibiotic resistance by directed evolution and structural analysis","volume":"8","author":"Orencia M.C.","year":"2001","unstructured":"Orencia M.C., , Yoon J.S., , Ness J.E., , Stemmer W.P.C., , Stevens R.C. 2001. Predicting the emergence of antibiotic resistance by directed evolution and structural analysis. Nat. Struct. Biol., 8:238\u2013242.","journal-title":"Nat. Struct. Biol."},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.4.657-686.2005"},{"key":"e_1_3_3_9_2","doi-asserted-by":"crossref","first-page":"3981","DOI":"10.1128\/AAC.46.12.3981-3983.2002","article-title":"Biochemical characterization of TEM-92 extended-spectrum beta-lactamase, a protein differing from TEM-52 in the signal peptide","volume":"46","author":"Perilli M.","year":"2002","unstructured":"Perilli M., , Segatore B., , De Massis M.R., , Pagani L., , Luzzaro F., , Rossolini G.M., , Amicosante G. 2002. Biochemical characterization of TEM-92 extended-spectrum beta-lactamase, a protein differing from TEM-52 in the signal peptide. Antimicrob. Agents Chemother., 46:3981\u20133983.","journal-title":"Antimicrob. Agents Chemother."},{"key":"e_1_3_3_10_2","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1128\/JCM.40.2.611-614.2002","article-title":"Molecular characterization of extended-spectrum \u00df-lactamases produced by nosocomial isolates of Enterobacteriaceae from an Italian nationwide survey","volume":"40","author":"Perilli M.","year":"2002","unstructured":"Perilli M., , Dell'Amico E., , Segatore B., , De Massis M.R., , Bianchi C., , Luzzaro F., , Rossolini G.M., , Toniolo A., , Nicoletti G., , Amicosante G. 2002. Molecular characterization of extended-spectrum \u00df-lactamases produced by nosocomial isolates of Enterobacteriaceae from an Italian nationwide survey. J. Clin. Microbiol., 40:611\u2013614.","journal-title":"J. Clin. Microbiol."},{"key":"e_1_3_3_11_2","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1128\/AAC.01028-07","article-title":"E240V substitution increases catalytic efficiency toward ceftazidime in a new natural TEM-type extended spectrum \u03b2-lactamase (TEM-149) from Enterobacter aerogenes and Serratia marcescens clinical isolates","volume":"52","author":"Perilli M.","year":"2007","unstructured":"Perilli M., , Celenza G., , De Santis F., , Pellegrini C., , Forcella C., , Rossolini G.M., , Stefani S., , Amicosante G. 2007. E240V substitution increases catalytic efficiency toward ceftazidime in a new natural TEM-type extended spectrum \u03b2-lactamase (TEM-149) from Enterobacter aerogenes and Serratia marcescens clinical isolates. Antimicrob. Agents Chemother., 52:915\u2013919.","journal-title":"Antimicrob. Agents Chemother."},{"key":"e_1_3_3_12_2","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1093\/jac\/dkm275","article-title":"Biochemical analysis of TEM-134, a new TEM-type extended-spectrum beta-lactamase variant produced in a Citrobacter koseri clinical isolate from an Italian hospital","volume":"60","author":"Perilli M.","year":"2007","unstructured":"Perilli M., , Celenza G., , Fiore M., , Segatore B., , Pellegrini C., , Luzzaro F., , Rossolini G.M., , Amicosante G. 2007. Biochemical analysis of TEM-134, a new TEM-type extended-spectrum beta-lactamase variant produced in a Citrobacter koseri clinical isolate from an Italian hospital. J. Antimicrob. Chemother., 60:877\u2013880.","journal-title":"J. Antimicrob. Chemother."},{"key":"e_1_3_3_13_2","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1016\/S0966-842X(98)01317-1","article-title":"B-lactamases: protein evolution in real time","volume":"6","author":"Petrosino J.","year":"1998","unstructured":"Petrosino J., , Cantu C.III, , Palzkill T. 1998. B-lactamases: protein evolution in real time. Trends Microbiol., 6:323\u2013327.","journal-title":"Trends Microbiol."},{"key":"e_1_3_3_14_2","doi-asserted-by":"crossref","first-page":"1821","DOI":"10.1128\/jb.178.7.1821-1828.1996","article-title":"Systematic mutagenesis of the active site omega loop of TEM-1 \u03b2-lactamase","volume":"178","author":"Petrosino J.F.","year":"1996","unstructured":"Petrosino J.F., , Palzkill T. 1996. Systematic mutagenesis of the active site omega loop of TEM-1 \u03b2-lactamase. J. Bacteriol., 178:1821\u20131828.","journal-title":"J. Bacteriol."},{"key":"e_1_3_3_15_2","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1006\/jmbi.1994.1756","article-title":"TEM \u03b2-lactamase mutants hydrolysing third-generation cephalosporins. A kinetic and molecular modelling analysis","volume":"244","author":"Raquet X.","year":"1994","unstructured":"Raquet X., , Lamotte-Brasseur J., , Fonz\u00e8 E., , Goussard S., , Courvalin P., , Fr\u00e8re J.M. 1994. TEM \u03b2-lactamase mutants hydrolysing third-generation cephalosporins. A kinetic and molecular modelling analysis. J. Mol. Biol., 244:625\u2013639.","journal-title":"J. Mol. Biol."},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2010.00222.x"},{"key":"e_1_3_3_17_2","doi-asserted-by":"crossref","first-page":"1833","DOI":"10.1128\/JCM.01467-09","article-title":"Citrobacter spp. simultaneously harbouring blaCTX-M, blaTEM, blaSHV, blaAMPC, and insertion sequences and orf513: an evolutionary phenomenon of recent concern for antibiotic resistance","volume":"48","author":"Shahid M.","year":"2010","unstructured":"Shahid M. 2010. Citrobacter spp. simultaneously harbouring blaCTX-M, blaTEM, blaSHV, blaAMPC, and insertion sequences and orf513: an evolutionary phenomenon of recent concern for antibiotic resistance. J. Clin. Microbiol., 48:1833\u20131838.","journal-title":"J. Clin. Microbiol."},{"key":"e_1_3_3_18_2","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1128\/AAC.46.1.196-202.2002","article-title":"Occurrence of extended-spectrum \u00df-lactamases in members of the family Enterobacteriaceae in Italy: implications for resistance to \u00df-lactams and other antimicrobial drugs","volume":"46","author":"Spanu T.","year":"2002","unstructured":"Spanu T., , Luzzaro F., , Perilli M., , Amicosante G., , Toniolo A., , Fadda G. the Italian ESBL study group. 2002. Occurrence of extended-spectrum \u00df-lactamases in members of the family Enterobacteriaceae in Italy: implications for resistance to \u00df-lactams and other antimicrobial drugs. Antimicrob. Agents Chemother., 46:196\u2013202.","journal-title":"Antimicrob. Agents Chemother."},{"key":"e_1_3_3_19_2","doi-asserted-by":"crossref","DOI":"10.1089\/mdr.2010.0055","article-title":"High prevalence of blaCTX-M estende-spectrum \u03b2-lactamase genes in Klebsiella pneumoniae isolates in a Tertiary Care Hospital: first report of blaSHV-12, blaSHV-31, blaSHV-38 and blaCTX-M-15 in Brazil","volume":"17","author":"Tollentino F.M.","year":"2011","unstructured":"Tollentino F.M., , Polotto M., , Nogueira M.L., , Lincopan N., , Neves P., , Mamizuka E.M., , Remeli G.A., , De Almeida M.T.G., , R\u00fabio F.G., , Nogueira M.C.L. 2011. High prevalence of blaCTX-M estende-spectrum \u03b2-lactamase genes in Klebsiella pneumoniae isolates in a Tertiary Care Hospital: first report of blaSHV-12, blaSHV-31, blaSHV-38 and blaCTX-M-15 in Brazil. Microb. Drug Resist., 17[Epub ahead of print]DOI: 10.1089\/mdr.2010.0055.","journal-title":"Microb. Drug Resist."},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm226"}],"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/mdr.2011.0059","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2011.0059","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2011.0059","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:26:28Z","timestamp":1777667188000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/mdr.2011.0059"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,12]]},"references-count":19,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2011,12]]}},"alternative-id":["10.1089\/mdr.2011.0059"],"URL":"https:\/\/doi.org\/10.1089\/mdr.2011.0059","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,12]]}}}