{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:10:47Z","timestamp":1787008247744,"version":"build-2736575974"},"reference-count":35,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2011,12,1]],"date-time":"2011-12-01T00:00:00Z","timestamp":1322697600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2011,12]]},"abstract":"\n The objectives of this study were to investigate the prevalence of plasmid-mediated quinolone resistance genes in a collection of 226 extended-spectrum \u03b2-lactamase (ESBL)\u2013producing Enterobacteriaceae isolates and characterize the\n qnr<\/jats:italic>\n -positive isolates. The rate of\n qnr<\/jats:italic>\n -positive isolates was 21.6% (49\/226), 49.5% for\n aac(6\u2032)-Ib-cr<\/jats:italic>\n (112\/226), and 1.7% for\n qepA1<\/jats:italic>\n (4\/226). Those isolates carried\n qnr<\/jats:italic>\n genes corresponding to types\n qnrB<\/jats:italic>\n (71.4%),\n qnrS1<\/jats:italic>\n (24.4%), and\n qnrA1<\/jats:italic>\n (18.3%). The distribution among bacterial species was as follows: 55.8% (19\/34) to\n Enterobacter cloacae<\/jats:italic>\n , 50% (28\/56) to\n Klebsiella pneumoniae<\/jats:italic>\n , and 1.4% (2\/136) to\n Escherichia coli<\/jats:italic>\n . The characterization of\n qnr<\/jats:italic>\n -positive isolates indicated the ESBL SHV-types as the most prevalent (81.6%), including the ESBLs SHV-12, SHV-5, and SHV-2a, followed by CTX-M-15 (44.9%) and TLA-1 (8.1%). In addition, for\n qnr<\/jats:italic>\n -positive isolates, the prevalence of\n aac(6\u2032)-Ib-cr<\/jats:italic>\n was 55.1%, but\n qepA<\/jats:italic>\n was not identified. Alterations at codons Ser-83 and Asp-87 in GyrA and at codons Ser-80 in ParC were observed in 69% and 80% of the\n qnr<\/jats:italic>\n -positive isolates, respectively. The analysis of the transconjugants revealed a cotransmission of\n bla<\/jats:italic>\n CTX-M-15<\/jats:sub>\n with\n qepA1<\/jats:italic>\n or\n aac(6\u2032)-Ib-cr<\/jats:italic>\n and\/or\n qnrA1<\/jats:italic>\n and\n bla<\/jats:italic>\n SHV-type<\/jats:sub>\n with\n qnrB5<\/jats:italic>\n and\n qnrB6<\/jats:italic>\n genes. To conclude, these findings indicate a high prevalence of\n qnr<\/jats:italic>\n and\n aac(6\u2032)-Ib-cr<\/jats:italic>\n among ESBL-producing isolates from Mexican hospitals and point to the wide spread of\n qnr<\/jats:italic>\n -like determinants associated to ESBLs SHV- and CTX-M-type in Mexican clinical isolates.\n <\/jats:p>","DOI":"10.1089\/mdr.2011.0086","type":"journal-article","created":{"date-parts":[[2011,8,11]],"date-time":"2011-08-11T11:33:00Z","timestamp":1313062380000},"page":"497-505","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":47,"title":["Prevalence and Characterization of Plasmid-Mediated Quinolone Resistance Genes in Extended-Spectrum \u03b2-Lactamase\u2013Producing Enterobacteriaceae Isolates in Mexico"],"prefix":"10.1177","volume":"17","author":[{"given":"Jesus","family":"Silva-Sanchez","sequence":"first","affiliation":[{"name":"CISEI","place":["M\u00e9xico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Humberto","family":"Barrios","sequence":"additional","affiliation":[{"name":"CISEI","place":["M\u00e9xico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Reyna-Flores","sequence":"additional","affiliation":[{"name":"CISEI","place":["M\u00e9xico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margarita","family":"Bello-Diaz","sequence":"additional","affiliation":[{"name":"CISEI","place":["M\u00e9xico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro","family":"Sanchez-Perez","sequence":"additional","affiliation":[{"name":"CISEI","place":["M\u00e9xico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa","family":"Rojas","sequence":"additional","affiliation":[{"name":"CISEI","place":["M\u00e9xico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bacterial Resistance","family":"Consortium","sequence":"additional","affiliation":[{"name":"Universidad Aut\u00f3noma de Nuevo Le\u00f3n","place":["M\u00e9xico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulises","family":"Garza-Ramos","sequence":"additional","affiliation":[{"name":"CISEI","place":["M\u00e9xico"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2011,12]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"crossref","first-page":"1067","DOI":"10.1086\/382354","article-title":"Nosocomial bacteremia and urinary tract infections caused by extended-spectrum beta-lactamase-producing Klebsiella pneumoniae with plasmids carrying both SHV-5 and TLA-1 genes","volume":"38","author":"Alcantar-Curiel D.","year":"2004","unstructured":"Alcantar-Curiel D., , Tinoco J.C., , Gayosso C., , Carlos A., , Daza C., , Perez-Prado M.C., , Salcido L., , Santos J.I., , Alpuche-Aranda C.M. 2004. 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