{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T22:42:58Z","timestamp":1782168178350,"version":"3.54.5"},"reference-count":23,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2012,4,1]],"date-time":"2012-04-01T00:00:00Z","timestamp":1333238400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:p>\n                    The intensive use of antibiotics in human and veterinary medicine, associated with mechanisms of bacterial genetic transfer, caused a selective pressure that contributed to the dissemination of antimicrobial resistance in different bacteria groups and throughout different ecosystems. Iberian wolf, due to his predatory and wild nature, may serve as an important indicator of environmental contamination with antimicrobial resistant bacteria. The aim of this study was to characterize the diversity of extended-spectrum \u03b2-lactamase (ESBL)-producing\n                    <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                    isolates within the fecal microbiota of Iberian wolf. Additionally, the identification of other associated resistance genes, phylogenetic groups, and the detection of virulence determinants were also focused on in this study. From 2008 to 2009, 237 fecal samples from Iberian wolf were collected in Portugal.\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    isolates with TEM-52, SHV-12, CTX-M-1, and CTX-M-14-type ESBLs were detected in 13 of these samples (5.5%). This study reveals the presence of ESBL-producing\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    isolates, in a wild ecosystem, which could be disseminated through the environment. Moreover, the presence of resistant genes in integrons and the existence of virulence determinants were shown. The association between antibiotic resistance and virulence determinants should be monitored, as it constitutes a serious public health problem.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2011.0145","type":"journal-article","created":{"date-parts":[[2011,12,20]],"date-time":"2011-12-20T09:44:04Z","timestamp":1324374244000},"page":"215-219","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":22,"title":["Iberian Wolf as a Reservoir of Extended-Spectrum \u03b2-Lactamase-Producing\n                    <i>Escherichia coli<\/i>\n                    of the TEM, SHV, and CTX-M Groups"],"prefix":"10.1177","volume":"18","author":[{"given":"Alexandre","family":"Gon\u00e7alves","sequence":"first","affiliation":[{"name":"University of Tr\u00e1s-os-Montes and Alto Douro","place":["Portugal"]},{"name":"University of Tr\u00e1s-os-Montes and Alto Douro","place":["Portugal"]},{"name":"Center for Animal Science and Veterinary","place":["Portugal"]},{"name":"University of Tr\u00e1s-os-Montes and Alto Douro","place":["Portugal"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilberto","family":"Igrejas","sequence":"additional","affiliation":[{"name":"University of Tr\u00e1s-os-Montes and Alto Douro","place":["Portugal"]},{"name":"University of Tr\u00e1s-os-Montes and Alto Douro","place":["Portugal"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hajer","family":"Radhouani","sequence":"additional","affiliation":[{"name":"University of Tr\u00e1s-os-Montes and Alto Douro","place":["Portugal"]},{"name":"University of Tr\u00e1s-os-Montes and Alto Douro","place":["Portugal"]},{"name":"Center for Animal Science and Veterinary","place":["Portugal"]},{"name":"University of Tr\u00e1s-os-Montes and Alto Douro","place":["Portugal"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanesa","family":"Estepa","sequence":"additional","affiliation":[{"name":"Universidad de La Rioja","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Pacheco","sequence":"additional","affiliation":[{"name":"University of Tr\u00e1s-os-Montes and Alto Douro","place":["Portugal"]},{"name":"University of Tr\u00e1s-os-Montes and Alto Douro","place":["Portugal"]},{"name":"Center for Animal Science and Veterinary","place":["Portugal"]},{"name":"University of Tr\u00e1s-os-Montes and Alto Douro","place":["Portugal"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo","family":"Monteiro","sequence":"additional","affiliation":[{"name":"University of Tr\u00e1s-os-Montes and Alto Douro","place":["Portugal"]},{"name":"University of Tr\u00e1s-os-Montes and Alto Douro","place":["Portugal"]},{"name":"Center for Animal Science and Veterinary","place":["Portugal"]},{"name":"University of Tr\u00e1s-os-Montes and Alto Douro","place":["Portugal"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco","family":"Brito","sequence":"additional","affiliation":[{"name":"Center for Animal Science and Veterinary","place":["Portugal"]},{"name":"University of Tr\u00e1s-os-Montes and Alto Douro","place":["Portugal"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Guerra","sequence":"additional","affiliation":[{"name":"Grupo Lobo, Lisbon Faculty of Science","place":["Portugal"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco","family":"Petrucci-Fonseca","sequence":"additional","affiliation":[{"name":"Grupo Lobo, Lisbon Faculty of Science","place":["Portugal"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Torres","sequence":"additional","affiliation":[{"name":"Universidad de La Rioja","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patr\u00edcia","family":"Poeta","sequence":"additional","affiliation":[{"name":"Center for Animal Science and Veterinary","place":["Portugal"]},{"name":"University of Tr\u00e1s-os-Montes and Alto Douro","place":["Portugal"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2012,4]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki370"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2005.03.018"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.10.4555-4558.2000"},{"key":"e_1_3_3_5_2","volume-title":"Informational Supplement. CLSI document M100-S21","author":"CLSI","year":"2011","unstructured":"CLSI. 2011. Performance Standards for Antimicrobial Susceptibility Testing. Twenty-first Informational Supplement. CLSI document M100-S21. Clinical and Laboratory Standards Institute: Wayne, PA."},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2009.03.029"},{"key":"e_1_3_3_7_2","doi-asserted-by":"crossref","first-page":"645","DOI":"10.3201\/eid1704.101009","article-title":"Complete sequence and molecular epidemiology of IncK epidemic plasmid encoding blaCTX-M-14","volume":"17","author":"Cottell J.L.","year":"2011","unstructured":"Cottell J.L., , Webber M.A., , Coldham N.G., , Taylor D.L., , Cerdeno-Tarraga A.M., , Hauser H., , Thomson N.R., , Woodward M.J., , Piddock L.J. 2011. Complete sequence and molecular epidemiology of IncK epidemic plasmid encoding blaCTX-M-14. Emerg. Infect. Dis., 17:645\u2013652.","journal-title":"Emerg. Infect. Dis."},{"key":"e_1_3_3_8_2","doi-asserted-by":"crossref","first-page":"2840","DOI":"10.1128\/JCM.02147-09","article-title":"Diversity of Escherichia coli strains producing extended-spectrum beta-lactamases in Spain: second nationwide study","volume":"48","author":"Diaz M.A.","year":"2010","unstructured":"Diaz M.A., , Hernandez-Bello J.R., , Rodriguez-Bano J., , Martinez-Martinez L., , Calvo J., , Blanco J., , Pascual A. 2010. Diversity of Escherichia coli strains producing extended-spectrum beta-lactamases in Spain: second nationwide study. J. Clin. Microbiol., 48:2840\u20132845.","journal-title":"J. Clin. Microbiol."},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki398"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2010.0598"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm316"},{"key":"e_1_3_3_12_2","doi-asserted-by":"crossref","first-page":"1823","DOI":"10.1128\/AAC.49.5.1823-1829.2005","article-title":"Integron content of extended-spectrum-beta-lactamase-producing Escherichia coli strains over 12 years in a single hospital in Madrid, Spain","volume":"49","author":"Machado E.","year":"2005","unstructured":"Machado E., , Canton R., , Baquero F., , Galan J.C., , Rollan A., , Peixe L., , Coque T.M. 2005. Integron content of extended-spectrum-beta-lactamase-producing Escherichia coli strains over 12 years in a single hospital in Madrid, Spain. Antimicrob. Agents Chemother., 49:1823\u20131829.","journal-title":"Antimicrob. Agents Chemother."},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm381"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkn179"},{"key":"e_1_3_3_15_2","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1093\/jac\/dkn428","article-title":"Replicon typing of plasmids in Escherichia coli producing extended-spectrum beta-lactamases","volume":"63","author":"Marcad\u00e9 G.","year":"2009","unstructured":"Marcad\u00e9 G., , Deschamps C., , Boyd A., , Gautier V., , Picard B., , Branger C., , Denamur E., , Arlet G. 2009. Replicon typing of plasmids in Escherichia coli producing extended-spectrum beta-lactamases. J. Antimicrob. Chemother., 63:67\u201371.","journal-title":"J. Antimicrob. Chemother."},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.4.657-686.2005"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/jobm.200900066"},{"key":"e_1_3_3_18_2","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1093\/jac\/dkp403","article-title":"Detection of Escherichia coli harbouring extended-spectrum {beta}-lactamases of the CTX-M classes in faecal samples of common buzzards (Buteo buteo)","volume":"65","author":"Radhouani H.","year":"2010","unstructured":"Radhouani H., , Pinto L., , Coelho C., , Goncalves A., , Sargo R., , Torres C., , Igrejas G., , Poeta P. 2010. Detection of Escherichia coli harbouring extended-spectrum {beta}-lactamases of the CTX-M classes in faecal samples of common buzzards (Buteo buteo) J. Antimicrob. Chemother., 65:171\u2013173.","journal-title":"J. Antimicrob. Chemother."},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.12.4445-4449.2002"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.10.3996-4001.2004"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkg168"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1080\/03079457.2011.599061"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.9.2233-2239.1995"},{"key":"e_1_3_3_24_2","doi-asserted-by":"crossref","first-page":"5204","DOI":"10.1128\/AAC.01706-08","article-title":"Spread of bla(CTX-M-14) is driven mainly by IncK plasmids disseminated among Escherichia coli phylogroups A, B1, and D in Spain","volume":"53","author":"Valverde A.","year":"2009","unstructured":"Valverde A., , Canton R., , Garcillan-Barcia M.P., , Novais A., , Galan J.C., , Alvarado A., , de la Cruz F., , Baquero F., , Coque T.M. 2009. Spread of bla(CTX-M-14) is driven mainly by IncK plasmids disseminated among Escherichia coli phylogroups A, B1, and D in Spain. Antimicrob. Agents Chemother., 53:5204\u20135212.","journal-title":"Antimicrob. Agents Chemother."}],"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/mdr.2011.0145","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2011.0145","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2011.0145","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:26:41Z","timestamp":1777667201000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/mdr.2011.0145"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,4]]},"references-count":23,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2012,4]]}},"alternative-id":["10.1089\/mdr.2011.0145"],"URL":"https:\/\/doi.org\/10.1089\/mdr.2011.0145","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,4]]}}}