{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T01:54:50Z","timestamp":1791251690427,"version":"4.1.0"},"reference-count":16,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2013,4,1]],"date-time":"2013-04-01T00:00:00Z","timestamp":1364774400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2013,4]]},"abstract":"\n A total of 788 clinical\n Enterobacteriaceae<\/jats:italic>\n were collected to describe the aminoglycoside-modifying genes (AME genes) and to characterize the plasmids that carry these genes. Among the 788 strains collected, 330 (41.8%) were aminoglycoside-resistant: 264\n Escherichia coli<\/jats:italic>\n (80%), 33\n Proteus mirabilis<\/jats:italic>\n (10%), 10\n Klebsiella pneumoniae<\/jats:italic>\n (3%), six\n K. oxytoca<\/jats:italic>\n (1.8%), five\n Enterobacter cloacae<\/jats:italic>\n (1.5%), three\n Morganella morganii<\/jats:italic>\n (0.9%), three\n Providencia stuartii<\/jats:italic>\n (0.9%), two\n Salmonella enterica<\/jats:italic>\n (0.6%), and one each\n Citrobacter freundii<\/jats:italic>\n ,\n C. koseri<\/jats:italic>\n ,\n Proteus vulgaris<\/jats:italic>\n , and\n Shigella sonnei<\/jats:italic>\n . The most affected aminoglycoside was streptomycin (92.7%), followed by kanamycin (26.3%), gentamicin (18%), tobramycin (16.9%), netilmicin (3.6%), and amikacin (1.5%). The AME genes found were\n aph(3\u2033)-Ib<\/jats:italic>\n (65.4%),\n ant(3\u2033)-Ia<\/jats:italic>\n (37.5%),\n aph(3\u2032)-Ia<\/jats:italic>\n (13.9%),\n aac(3)-IIa<\/jats:italic>\n (12.4%),\n aac(6\u2032)-Ib<\/jats:italic>\n (4.2%),\n ant(2\u2033)-Ia<\/jats:italic>\n (3.6%), and\n aph(3\u2032)-IIa<\/jats:italic>\n (1.2%). Thirty-four percent of the strains showed more than one enzyme. The most frequent association was\n ant(3\u2033)-Ia<\/jats:italic>\n plus\n aph(3\u2033)-Ib<\/jats:italic>\n (35 strains). From 66 selected AME genes, 24 were plasmid located: 12\n aac(3)-IIa<\/jats:italic>\n , six\n aph(3\u2032)-Ia<\/jats:italic>\n , three\n ant(3\u2033)-Ia<\/jats:italic>\n , two\n ant(2\u2033)-Ia<\/jats:italic>\n , and one\n aac(6\u2032)-Ib<\/jats:italic>\n . These genes were located in plasmids belonging to incompatibility groups F, FIA, FIB, or HI2. In conclusion, the AME genes involved in aminoglycoside-clinical resistance were\n aac(3)-IIa<\/jats:italic>\n ,\n aac(6\u2032)-Ib<\/jats:italic>\n , and\n ant(2\u2033)-Ia<\/jats:italic>\n , genes that confer resistance to tobramycin, gentamicin, and amikacin.\n <\/jats:p>","DOI":"10.1089\/mdr.2012.0125","type":"journal-article","created":{"date-parts":[[2012,12,3]],"date-time":"2012-12-03T11:23:55Z","timestamp":1354533835000},"page":"94-99","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":77,"title":["Characterization of Aminoglycoside-Modifying Enzymes in\n Enterobacteriaceae<\/i>\n Clinical Strains and Characterization of the Plasmids Implicated in Their Diffusion"],"prefix":"10.1177","volume":"19","author":[{"given":"Elisenda","family":"Mir\u00f3","sequence":"first","affiliation":[{"name":"Hospital de la Santa Creu i Sant Pau","place":["Spain"]},{"name":"Institut d'Investigaci\u00f3 Biom\u00e8dica Sant Pau","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federico","family":"Gr\u00fcnbaum","sequence":"additional","affiliation":[{"name":"Hospital de la Santa Creu i Sant Pau","place":["Spain"]},{"name":"Universitat Aut\u00f2noma de Barcelona","place":["Spain"]},{"name":"Institut d'Investigaci\u00f3 Biom\u00e8dica Sant Pau","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"G\u00f3mez","sequence":"additional","affiliation":[{"name":"Institut d'Investigaci\u00f3 Biom\u00e8dica Sant Pau","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alba","family":"Rivera","sequence":"additional","affiliation":[{"name":"Hospital de la Santa Creu i Sant Pau","place":["Spain"]},{"name":"Institut d'Investigaci\u00f3 Biom\u00e8dica Sant Pau","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz","family":"Mirelis","sequence":"additional","affiliation":[{"name":"Hospital de la Santa Creu i Sant Pau","place":["Spain"]},{"name":"Universitat Aut\u00f2noma de Barcelona","place":["Spain"]},{"name":"Institut d'Investigaci\u00f3 Biom\u00e8dica Sant Pau","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pere","family":"Coll","sequence":"additional","affiliation":[{"name":"Hospital de la Santa Creu i Sant Pau","place":["Spain"]},{"name":"Universitat Aut\u00f2noma de Barcelona","place":["Spain"]},{"name":"Institut d'Investigaci\u00f3 Biom\u00e8dica Sant Pau","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ferran","family":"Navarro","sequence":"additional","affiliation":[{"name":"Hospital de la Santa Creu i Sant Pau","place":["Spain"]},{"name":"Universitat Aut\u00f2noma de Barcelona","place":["Spain"]},{"name":"Institut d'Investigaci\u00f3 Biom\u00e8dica Sant Pau","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2013,4]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2005.03.018"},{"key":"e_1_3_3_3_2","volume-title":"Performance standards for antimicrobial susceptibility testing: twentieth informational supplement M100-S20","author":"Clinical, Laboratory Standards Institute","year":"2010","unstructured":"Clinical, Laboratory Standards Institute. 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