{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T21:44:40Z","timestamp":1790372680760,"version":"4.1.0"},"reference-count":25,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2013,8,1]],"date-time":"2013-08-01T00:00:00Z","timestamp":1375315200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:p>\n                    We analyzed extended-spectrum \u03b2-lactamase (ESBL)-producing\n                    <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                    (226) and\n                    <jats:italic toggle=\"yes\">Klebsiella pneumoniae<\/jats:italic>\n                    (53) collected from urine specimens during 2005\u20132009 for the presence of ESBL genes, class 1 integrons, and characterization of gene cassettes. TEM and CTX-M \u03b2-lactamase genes were the most prevalent. One hundred and forty-four\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    and 35\n                    <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                    were positive for the class 1 integrase gene; among them, 99\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    and 14\n                    <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                    detected gene cassettes. Gene cassette regions were identified by restriction fragment length polymorphism and DNA sequencing analysis. Eleven distinct gene cassette arrays were found in\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    isolates, and seven distinct gene cassette arrays were found in\n                    <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                    isolates. The cassette array\n                    <jats:italic toggle=\"yes\">aacA4<\/jats:italic>\n                    -\n                    <jats:italic toggle=\"yes\">catB8<\/jats:italic>\n                    -\n                    <jats:italic toggle=\"yes\">aadA1<\/jats:italic>\n                    was first found in\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">dfrA1<\/jats:italic>\n                    -orfC was first reported in\n                    <jats:italic toggle=\"yes\">K. pneumonia<\/jats:italic>\n                    e. Most of the gene cassettes found in this class 1 integrons were for aminoglycoside resistance. Enterobacterial repetitive intergenic consensus\u2013polymerase chain reaction fingerprint patterns revealed the isolates carrying gene cassettes were genetically unrelated. In conclusion, we studied the class 1 integrons among urinary isolates of ESBL-producing\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                    in Southern China during the past 5 years and found that class 1 integrons were widely disseminated and played a major role in antibiotic resistance.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2012.0130","type":"journal-article","created":{"date-parts":[[2013,4,10]],"date-time":"2013-04-10T08:34:03Z","timestamp":1365582843000},"page":"289-294","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":11,"title":["Class 1 Integrons in Urinary Isolates of Extended-Spectrum \u03b2-Lactamase-Producing\n                    <i>Escherichia coli<\/i>\n                    and\n                    <i>Klebsiella pneumoniae<\/i>\n                    in Southern China During the Past Five Years"],"prefix":"10.1177","volume":"19","author":[{"given":"Jingjing","family":"Sun","sequence":"first","affiliation":[{"name":"Southern Medical University","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fen","family":"Zheng","sequence":"additional","affiliation":[{"name":"Southern Medical University","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengping","family":"Wang","sequence":"additional","affiliation":[{"name":"Southern Medical University","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuihai","family":"Wu","sequence":"additional","affiliation":[{"name":"Southern Medical University","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Wang","sequence":"additional","affiliation":[{"name":"Southern Medical University","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Chen","sequence":"additional","affiliation":[{"name":"Southern Medical University","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shouyi","family":"Yu","sequence":"additional","affiliation":[{"name":"Southern Medical University","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongyu","family":"Rui","sequence":"additional","affiliation":[{"name":"Southern Medical University","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2013,8]]},"reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1093\/jac\/dkf069","article-title":"Identification of PSE and OXA beta-lactamase genes in Pseudomonas aeruginosa using PCR-restriction fragment length polymorphism","volume":"50","author":"Bert F.","year":"2002","unstructured":"Bert F., , Branger C., , Lambert-Zechovsky N. 2002. Identification of PSE and OXA beta-lactamase genes in Pseudomonas aeruginosa using PCR-restriction fragment length polymorphism. J. Antimicrob. Chemother., 50:11\u201318.","journal-title":"J. Antimicrob. Chemother."},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"3939","DOI":"10.1128\/AAC.01831-10","article-title":"Genetic context and structural diversity of class 1 integrons from human commensal bacteria in a hospital intensive care unit","volume":"55","author":"Betteridge T.","year":"2011","unstructured":"Betteridge T., , Partridge S.R., , Iredell J.R., , Stokes H.W. 2011. Genetic context and structural diversity of class 1 integrons from human commensal bacteria in a hospital intensive care unit. Antimicrob. Agents Chemother., 55:3939\u20133943.","journal-title":"Antimicrob. Agents Chemother."},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"207","DOI":"10.4103\/0255-0857.66472","article-title":"Observation on integron carriage among clinical isolates of Klebsiella pneumoniae producing extended-spectrum beta-lactamases","volume":"28","author":"Bhattacharjee A.","year":"2010","unstructured":"Bhattacharjee A., , Sen M.R., , Prakash P., , Gaur A., , Anupurba S., , Nath G. 2010. Observation on integron carriage among clinical isolates of Klebsiella pneumoniae producing extended-spectrum beta-lactamases. Indian J. Med. Microbiol., 28:207\u2013210.","journal-title":"Indian J. Med. Microbiol."},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1111\/j.1472-765X.2010.02926.x","article-title":"Antibiotic resistance patterns and sequencing of class I integron from uropathogenic Escherichia coli in Lebanon","volume":"51","author":"El-Najjar N.G.","year":"2010","unstructured":"El-Najjar N.G., , Farah M.J., , Hashwa F.A., , Tokajian S.T. 2010. Antibiotic resistance patterns and sequencing of class I integron from uropathogenic Escherichia coli in Lebanon. Lett. Appl. Microbiol., 51:456\u2013461.","journal-title":"Lett. Appl. Microbiol."},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1016\/j.ijantimicag.2008.06.019","article-title":"Novel cassette arrays of integrons in clinical strains of Enterobacteriaceae in China","volume":"32","author":"Gu B.","year":"2008","unstructured":"Gu B., , Pan S., , Wang T., , Zhao W., , Mei Y., , Huang P., , Tong M. 2008. Novel cassette arrays of integrons in clinical strains of Enterobacteriaceae in China. Int. J. Antimicrob. Agents, 32:529\u2013533.","journal-title":"Int. J. Antimicrob. Agents"},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1111\/j.1472-765X.2011.03059.x","article-title":"Genetic diversity analyses of class 1 integrons and their associated antimicrobial resistance genes in Enterobacteriaceae strains recovered from aquatic habitats in China","volume":"52","author":"Guo X.","year":"2011","unstructured":"Guo X., , Xia R., , Han N., , Xu H. 2011. Genetic diversity analyses of class 1 integrons and their associated antimicrobial resistance genes in Enterobacteriaceae strains recovered from aquatic habitats in China. Lett. Appl. Microbiol., 52:667\u2013675.","journal-title":"Lett. Appl. Microbiol."},{"key":"e_1_3_2_8_2","first-page":"37","article-title":"Detailed mechanism for transposition by TnpA transposase involves DNA shape rather than direct protein-DNA recognition to generate an active nucleoprotein complex","volume":"1","author":"Ho P.S.","year":"2009","unstructured":"Ho P.S. 2009. Detailed mechanism for transposition by TnpA transposase involves DNA shape rather than direct protein-DNA recognition to generate an active nucleoprotein complex. F1000 Biol. Rep., 1:37.","journal-title":"F1000 Biol. Rep."},{"key":"e_1_3_2_9_2","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1016\/j.ijantimicag.2005.11.020","article-title":"Characterisation of antimicrobial resistance patterns and class 1 integrons among Escherichia coli and Salmonella enterica serovar Choleraesuis strains isolated from humans and swine in Taiwan","volume":"27","author":"Hsu S.C.","year":"2006","unstructured":"Hsu S.C., , Chiu T.H., , Pang J.C., , Hsuan-Yuan C.H., , Chang G.N., , Tsen H.Y. 2006. Characterisation of antimicrobial resistance patterns and class 1 integrons among Escherichia coli and Salmonella enterica serovar Choleraesuis strains isolated from humans and swine in Taiwan. Int. J. Antimicrob. Agents, 27:383\u2013391.","journal-title":"Int. J. Antimicrob. Agents"},{"key":"e_1_3_2_10_2","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1111\/j.1348-0421.2009.00168.x","article-title":"Characterization of integrons and antimicrobial resistance genes in clinical isolates of Gram-negative bacteria from Palestinian hospitals","volume":"53","author":"Hussein A.I.","year":"2009","unstructured":"Hussein A.I., , Ahmed A.M., , Sato M., , Shimamoto T. 2009. Characterization of integrons and antimicrobial resistance genes in clinical isolates of Gram-negative bacteria from Palestinian hospitals. Microbiol. Immunol., 53:595\u2013602.","journal-title":"Microbiol. Immunol."},{"key":"e_1_3_2_11_2","doi-asserted-by":"crossref","first-page":"794","DOI":"10.1128\/AAC.49.2.794-797.2005","article-title":"The aadB gene cassette is associated with blaSHV genes in Klebsiella species producing extended-spectrum beta-lactamases","volume":"49","author":"Jones L.A.","year":"2005","unstructured":"Jones L.A., , McIver C.J., , Kim M.J., , Rawlinson W.D., , White P.A. 2005. The aadB gene cassette is associated with blaSHV genes in Klebsiella species producing extended-spectrum beta-lactamases. Antimicrob. Agents Chemother., 49:794\u2013797.","journal-title":"Antimicrob. Agents Chemother."},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki076"},{"key":"e_1_3_2_13_2","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1007\/978-1-60327-853-9_6","article-title":"The integron\/gene cassette system: an active player in bacterial adaptation","volume":"532","author":"Labbate M.","year":"2009","unstructured":"Labbate M., , Case R.J., , Stokes H.W. 2009. The integron\/gene cassette system: an active player in bacterial adaptation. Methods Mol. Biol., 532:103\u2013125.","journal-title":"Methods Mol. Biol."},{"key":"e_1_3_2_14_2","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1128\/AAC.00988-10","article-title":"Use of inverse PCR for analysis of class 1 integrons carrying an unusual 3\u2032 conserved segment structure","volume":"55","author":"Lee M.F.","year":"2011","unstructured":"Lee M.F., , Peng C.F., , Hsu H.J., , Toh H.S. 2011. Use of inverse PCR for analysis of class 1 integrons carrying an unusual 3\u2032 conserved segment structure. Antimicrob. Agents Chemother., 55:943\u2013945.","journal-title":"Antimicrob. Agents Chemother."},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.diagmicrobio.2008.09.002","article-title":"Spread of CTX-M-type extended-spectrum beta-lactamases among bloodstream isolates of Escherichia coli and Klebsiella pneumoniae from a Korean hospital","volume":"63","author":"Lee S.G.","year":"2009","unstructured":"Lee S.G., , Jeong S.H., , Lee H., , Kim C.K., , Lee Y., , Koh E., , Chong Y., , Lee K. 2009. Spread of CTX-M-type extended-spectrum beta-lactamases among bloodstream isolates of Escherichia coli and Klebsiella pneumoniae from a Korean hospital. Diagn. Microbiol. Infect. Dis., 63:76\u201380.","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.1.185"},{"key":"e_1_3_2_17_2","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1016\/j.jmii.2010.08.012","article-title":"Antimicrobial susceptibilities of urinary extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae to fosfomycin and nitrofurantoin in a teaching hospital in Taiwan","volume":"44","author":"Liu H.Y.","year":"2011","unstructured":"Liu H.Y., , Lin H.C., , Lin Y.C., , Yu S.H., , Wu W.H., , Lee Y.J. 2011. Antimicrobial susceptibilities of urinary extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae to fosfomycin and nitrofurantoin in a teaching hospital in Taiwan. J. Microbiol. Immunol. Infect., 44:364\u2013368.","journal-title":"J. Microbiol. Immunol. Infect."},{"key":"e_1_3_2_18_2","doi-asserted-by":"crossref","first-page":"2201","DOI":"10.1128\/AAC.01389-06","article-title":"Preservation of integron types among Enterobacteriaceae producing extended-spectrum beta-lactamases in a Spanish hospital over a 15-year period (1988 to 2003)","volume":"51","author":"Machado E.","year":"2007","unstructured":"Machado E., , Ferreira J., , Novais A., , Peixe L., , Canton R., , Baquero F., , Coque T.M. 2007. Preservation of integron types among Enterobacteriaceae producing extended-spectrum beta-lactamases in a Spanish hospital over a 15-year period (1988 to 2003) Antimicrob. Agents Chemother, 51:2201\u20132204.","journal-title":"Antimicrob. Agents Chemother"},{"key":"e_1_3_2_19_2","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1093\/jac\/42.6.689","article-title":"Class I integrons in Gram-negative isolates from different European hospitals and association with decreased susceptibility to multiple antibiotic compounds","volume":"42","author":"Martinez-Freijo P.","year":"1998","unstructured":"Martinez-Freijo P., , Fluit A.C., , Schmitz F.J., , Grek V.S., , Verhoef J., , Jones M.E. 1998. Class I integrons in Gram-negative isolates from different European hospitals and association with decreased susceptibility to multiple antibiotic compounds. J. Antimicrob. Chemother., 42:689\u2013696.","journal-title":"J. Antimicrob. Chemother."},{"key":"e_1_3_2_20_2","doi-asserted-by":"crossref","first-page":"2492","DOI":"10.1128\/AAC.00033-09","article-title":"Integron mobilization unit as a source of mobility of antibiotic resistance genes","volume":"53","author":"Poirel L.","year":"2009","unstructured":"Poirel L., , Carrer A., , Pitout J.D., , Nordmann P. 2009. Integron mobilization unit as a source of mobility of antibiotic resistance genes. Antimicrob. Agents Chemother., 53:2492\u20132498.","journal-title":"Antimicrob. Agents Chemother."},{"key":"e_1_3_2_21_2","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.3201\/eid1206.051596","article-title":"Class 1 integrons in resistant Escherichia coli and Klebsiella spp., US hospitals","volume":"12","author":"Rao A.N.","year":"2006","unstructured":"Rao A.N., , Barlow M., , Clark L.A., , Boring J.R., , Tenover F.C., , McGowan J.J. 2006. Class 1 integrons in resistant Escherichia coli and Klebsiella spp., US hospitals. Emerg. Infect. Dis., 12:1011\u20131014.","journal-title":"Emerg. Infect. Dis."},{"key":"e_1_3_2_22_2","doi-asserted-by":"crossref","first-page":"3140","DOI":"10.1128\/AAC.01529-10","article-title":"Dissemination of multiple drug resistance genes by class 1 integrons in Klebsiella pneumoniae isolates from four countries: a comparative study","volume":"55","author":"Roy C.","year":"2011","unstructured":"Roy C., , Ingold A., , Vanegas N., , Martinez E., , Merlino J., , Merkier A.K., , Castro M., , Gonzalez R.G., , Borthagaray G., , Centron D., , Bello T.H., , Marquez C.M., , Stokes H.W. 2011. Dissemination of multiple drug resistance genes by class 1 integrons in Klebsiella pneumoniae isolates from four countries: a comparative study. Antimicrob. Agents Chemother., 55:3140\u20133149.","journal-title":"Antimicrob. Agents Chemother."},{"key":"e_1_3_2_23_2","doi-asserted-by":"crossref","first-page":"5407","DOI":"10.1128\/JCM.41.12.5407-5413.2003","article-title":"PCR typing of genetic determinants for metallo-beta-lactamases and integrases carried by gram-negative bacteria isolated in Japan, with focus on the class 3 integron","volume":"41","author":"Shibata N.","year":"2003","unstructured":"Shibata N., , Doi Y., , Yamane K., , Yagi T., , Kurokawa H., , Shibayama K., , Kato H., , Kai K., , Arakawa Y. 2003. PCR typing of genetic determinants for metallo-beta-lactamases and integrases carried by gram-negative bacteria isolated in Japan, with focus on the class 3 integron. J. Clin. Microbiol., 41:5407\u20135413.","journal-title":"J. Clin. Microbiol."},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.24.6823"},{"key":"e_1_3_2_25_2","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1007\/s00284-009-9405-z","article-title":"Four novel resistance integron gene-cassette occurrences in bacterial isolates from Zhenjiang, China","volume":"59","author":"Xu H.","year":"2009","unstructured":"Xu H., , Su Z., , Wang S., , Dai X., , Chen J., , Kong F., , Li Y., , Peng S., , Shao Q., , Lu L., , Ezaki T. 2009. Four novel resistance integron gene-cassette occurrences in bacterial isolates from Zhenjiang, China. Curr. Microbiol., 59:113\u2013117.","journal-title":"Curr. Microbiol."},{"key":"e_1_3_2_26_2","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1111\/j.1745-7270.2007.00304.x","article-title":"Incidence of extended-spectrum beta-lactamases and characterization of integrons in extended-spectrum beta-lactamase-producing Klebsiella pneumoniae isolated in Shantou, China","volume":"39","author":"Yao F.","year":"2007","unstructured":"Yao F., , Qian Y., , Chen S., , Wang P., , Huang Y. 2007. Incidence of extended-spectrum beta-lactamases and characterization of integrons in extended-spectrum beta-lactamase-producing Klebsiella pneumoniae isolated in Shantou, China. Acta Biochim. Biophys. Sin (Shanghai), 39:527\u2013532.","journal-title":"Acta Biochim. Biophys. Sin (Shanghai)"}],"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/mdr.2012.0130","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2012.0130","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2012.0130","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:27:21Z","timestamp":1777667241000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/mdr.2012.0130"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,8]]},"references-count":25,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2013,8]]}},"alternative-id":["10.1089\/mdr.2012.0130"],"URL":"https:\/\/doi.org\/10.1089\/mdr.2012.0130","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,8]]}}}