{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:24:03Z","timestamp":1791307443485,"version":"4.3.1"},"reference-count":35,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2013,1,30]],"date-time":"2013-01-30T00:00:00Z","timestamp":1359504000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2013,6,1]]},"abstract":"<jats:p>\n                    This study was conducted to investigate the molecular epidemiology of extended spectrum \u03b2-lactamase (ESBL)-producing\n                    <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                    in community-acquired (urinary tract) infections (CA-UTI) in Tunisia. Between January 2007 and December 2009, 15\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    isolates were collected at the laboratory of microbiology of Charles Nicolle Hospital of Tunis. Microbial identification was done with conventional methods. Antibiotic susceptibility was determined by disk diffusion method and ESBL detection was done with double-disk synergy test. ESBL typing was performed by polymerase chain reaction (PCR) and sequencing. Phylogenetic groups, virulence factors, and sequence type (ST)131 were determined by PCR. Genetic relatedness between strains was examined by pulsed-field gel electrophoresis (PFGE) after restriction with\n                    <jats:italic toggle=\"yes\">Xba<\/jats:italic>\n                    I. The prevalence of ESBL-producing\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    in CA-UTI was 0.046%. The majority of isolates were multidrug resistant. ESBL types were CTX-M-15 (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    =13) and SHV-12 (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    =2). The most common phylogenetic group was B2 (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    =11) and virulence score was greater than or equal to 9 in nine strains. PFGE revealed 12 clusters. The majority of isolates (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    =14) belonged to ST131 clone and 11 of them were CTX-M producers. In conclusion, this is the first detailed documentation of CA-ESBLs producing\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    in Tunisia. Of particular concern is the emergence in our community of the highly diffusing CTX-M-15-B2-ST131\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    clone, which requires strengthening surveillance measures to countervail this emergent public health problem.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2012.0172","type":"journal-article","created":{"date-parts":[[2013,1,30]],"date-time":"2013-01-30T09:26:44Z","timestamp":1359538004000},"page":"231-236","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":21,"title":["Characterization of Extended Spectrum \u03b2-Lactamase-Producing\n                    <i>Escherichia coli<\/i>\n                    in Community-Acquired Urinary Tract Infections in Tunisia"],"prefix":"10.1177","volume":"19","author":[{"given":"Samia","family":"Hammami","sequence":"first","affiliation":[{"name":"Laboratoire de Recherche \u201cR\u00e9sistance aux Antimicrobiens,\u201d","place":["Tunisia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mabrouka","family":"Saidani","sequence":"additional","affiliation":[{"name":"Laboratoire de Recherche \u201cR\u00e9sistance aux Antimicrobiens,\u201d","place":["Tunisia"]},{"name":"Hopital Charles Nicolle","place":["Tunisia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sana","family":"Ferjeni","sequence":"additional","affiliation":[{"name":"Laboratoire de Recherche \u201cR\u00e9sistance aux Antimicrobiens,\u201d","place":["Tunisia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ines","family":"Aissa","sequence":"additional","affiliation":[{"name":"Laboratoire de Recherche \u201cR\u00e9sistance aux Antimicrobiens,\u201d","place":["Tunisia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amin","family":"Slim","sequence":"additional","affiliation":[{"name":"Laboratoire de Recherche \u201cR\u00e9sistance aux Antimicrobiens,\u201d","place":["Tunisia"]},{"name":"Hopital Charles Nicolle","place":["Tunisia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilhem","family":"Boutiba-Ben Boubaker","sequence":"additional","affiliation":[{"name":"Laboratoire de Recherche \u201cR\u00e9sistance aux Antimicrobiens,\u201d","place":["Tunisia"]},{"name":"Hopital Charles Nicolle","place":["Tunisia"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2013,1,30]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2008.04.009"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.11.3506-3514.2003"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp108"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-011-1471-z"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-011-9930-4"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.1.1-14.2004"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.3855\/jidc.1490"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2004.09.013"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.5.1912-1918.2003"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1186\/1824-7288-37-3"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.10.4555-4558.2000"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp194"},{"key":"e_1_3_3_14_2","first-page":"M100","volume-title":"Performance Standards for Antimicrobial Susceptibility Testing. 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