{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:24:31Z","timestamp":1777922671196,"version":"3.51.4"},"reference-count":33,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2013,8,1]],"date-time":"2013-08-01T00:00:00Z","timestamp":1375315200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Fluconazole resistance of\n                    <jats:italic toggle=\"yes\">Candida albicans<\/jats:italic>\n                    has been reported to be the result of one or more specific point mutations in\n                    <jats:italic toggle=\"yes\">ERG11<\/jats:italic>\n                    gene. In this study, we amplified and sequenced the entire\n                    <jats:italic toggle=\"yes\">ERG11<\/jats:italic>\n                    coding sequence of 72 isolates of\n                    <jats:italic toggle=\"yes\">C. albicans<\/jats:italic>\n                    to search for possible mutations. Twenty-seven silent mutations and 14 missense mutations were identified. While the mutations K342R and V437I were found as single-amino-acid changes in Erg11p, other mutations were detected simultaneously in individual isolates. Several different clinical isolates had the same pattern of multiple amino acid alternations: (1) A114S with Y257H was identified in 11 resistant and 3 susceptible dose-dependent isolates without any other silent mutation and may be associated with resistance; (2) Y132H combined with G450E was identified in two fluconazole-resistant isolates and is known to contribute to resistance; and (3) the coexistence of D116E, K128T, Y132H, and G465S was first described in five reduced-susceptibility isolates, but the correlation of this pattern with resistance is still uncertain. These data indicate that multiple amino acid substitutions in Erg11p were found frequently in clinical isolates and may be associated with fluconazole resistance.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2012.0204","type":"journal-article","created":{"date-parts":[[2013,3,13]],"date-time":"2013-03-13T09:44:05Z","timestamp":1363167845000},"page":"266-273","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":33,"title":["<i>In Vitro<\/i>\n                    Fluconazole Susceptibility of 1,903 Clinical Isolates of\n                    <i>Candida albicans<\/i>\n                    and the Identification of\n                    <i>ERG11<\/i>\n                    Mutations"],"prefix":"10.1177","volume":"19","author":[{"given":"Ying","family":"Ying","sequence":"first","affiliation":[{"name":"Nanchang University","place":["People's Republic of China"]},{"name":"Nanchang University","place":["People's Republic of China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingjie","family":"Zhao","sequence":"additional","affiliation":[{"name":"Nanchang University","place":["People's Republic of China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuefei","family":"Hu","sequence":"additional","affiliation":[{"name":"Nanchang University","place":["People's Republic of China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenyu","family":"Cai","sequence":"additional","affiliation":[{"name":"Nanchang University","place":["People's Republic of China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Liu","sequence":"additional","affiliation":[{"name":"Nanchang University","place":["People's Republic of China"]},{"name":"Children's Hospital of Jiangxi Province","place":["People's Republic of China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guilin","family":"Jin","sequence":"additional","affiliation":[{"name":"Nanchang University","place":["People's Republic of China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jieyu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Nanchang University","place":["People's Republic of China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingyi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Nanchang University","place":["People's Republic of China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinhui","family":"Liu","sequence":"additional","affiliation":[{"name":"Nanchang University","place":["People's Republic of China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaotian","family":"Huang","sequence":"additional","affiliation":[{"name":"Nanchang University","place":["People's Republic of China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2013,8]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.5144\/0256-4947.2001.178"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2005.01310.x"},{"key":"e_1_3_3_4_2","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/j.ijantimicag.2005.12.005","article-title":"Resistance mechanisms in fluconazole-resistant Candida albicans isolates from vaginal candidiasis","volume":"27","author":"Cernicka J.","year":"2006","unstructured":"Cernicka J., , Subik J. 2006. Resistance mechanisms in fluconazole-resistant Candida albicans isolates from vaginal candidiasis. Int. J. Antimicrob. Agents, 27:403\u2013408.","journal-title":"Int. J. Antimicrob. Agents"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.18.5385-5394.2001"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.6.2124-2131.2004"},{"key":"e_1_3_3_7_2","doi-asserted-by":"crossref","first-page":"1246","DOI":"10.1248\/bpb.30.1246","article-title":"Identification of Y118 amino acid residue in Candida albicans sterol 14alpha-demethylase associated with the enzyme activity and selective antifungal activity of azole analogues","volume":"30","author":"Chen S.H.","year":"2007","unstructured":"Chen S.H., , Sheng C.Q., , Xu X.H., , Jiang Y.Y., , Zhang W.N., , He C. 2007. Identification of Y118 amino acid residue in Candida albicans sterol 14alpha-demethylase associated with the enzyme activity and selective antifungal activity of azole analogues. Biol. Pharm. Bull., 30:1246\u20131253.","journal-title":"Biol. Pharm. Bull."},{"issue":"10","key":"e_1_3_3_8_2","doi-asserted-by":"crossref","first-page":"2715","DOI":"10.1099\/00221287-145-10-2715","article-title":"Multiple amino acid substitutions in lanosterol 14alpha-demethylase contribute to azole resistance in Candida albicans","volume":"145","author":"Favre B.","year":"1999","unstructured":"Favre B., , Didmon M., , Ryder N.S. 1999. Multiple amino acid substitutions in lanosterol 14alpha-demethylase contribute to azole resistance in Candida albicans. Microbiology, 145,Pt 10:2715\u20132725.","journal-title":"Microbiology"},{"key":"e_1_3_3_9_2","doi-asserted-by":"crossref","first-page":"544","DOI":"10.3760\/cma.j.issn.0366-6999.2010.05.007","article-title":"Relationship between antifungal resistance of fluconazole resistant Candida albicans and mutations in ERG11 gene","volume":"123","author":"Feng L.J.","year":"2010","unstructured":"Feng L.J., , Wan Z., , Wang X.H., , Li R.Y., , Liu W. 2010. Relationship between antifungal resistance of fluconazole resistant Candida albicans and mutations in ERG11 gene. Chin. Med. J. (Engl.), 123:544\u2013548.","journal-title":"Chin. Med. J. (Engl.)"},{"key":"e_1_3_3_10_2","doi-asserted-by":"crossref","first-page":"3126","DOI":"10.1128\/AAC.00118-10","article-title":"Correlation between azole susceptibilities, genotypes, and ERG11 mutations in Candida albicans isolates associated with vulvovaginal candidiasis in China","volume":"54","author":"Ge S.H.","year":"2010","unstructured":"Ge S.H., , Wan Z., , Li J., , Xu J., , Li R.Y., , Bai F.Y. 2010. Correlation between azole susceptibilities, genotypes, and ERG11 mutations in Candida albicans isolates associated with vulvovaginal candidiasis in China. Antimicrob. Agents Chemother., 54:3126\u20133131.","journal-title":"Antimicrob. 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Acta Helv."},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/35.1.103"},{"key":"e_1_3_3_14_2","first-page":"360","article-title":"Synergisic effects of terbinafine combined with fluconazole or itraconazole on stable fluconazole-resistant Candida albicans induced by fluconazole in vitro","volume":"26","author":"Jiang W.","year":"2006","unstructured":"Jiang W., , Tan S., , Jiang G., , Chen Q. 2006. Synergisic effects of terbinafine combined with fluconazole or itraconazole on stable fluconazole-resistant Candida albicans induced by fluconazole in vitro. Chin. J. Microbiol. Immunol., 26:360\u2013364.","journal-title":"Chin. J. Microbiol. Immunol."},{"key":"e_1_3_3_15_2","doi-asserted-by":"crossref","first-page":"2985","DOI":"10.1128\/AAC.44.11.2985-2990.2000","article-title":"Genetic analysis of azole resistance in the Darlington strain of Candida albicans","volume":"44","author":"Kakeya H.","year":"2000","unstructured":"Kakeya H., , Miyazaki Y., , Miyazaki H., , Nyswaner K., , Grimberg B., , Bennett J.E. 2000. Genetic analysis of azole resistance in the Darlington strain of Candida albicans. Antimicrob. Agents Chemother., 44:2985\u20132990.","journal-title":"Antimicrob. Agents Chemother."},{"key":"e_1_3_3_16_2","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1006\/bbrc.1999.1136","article-title":"The G464S amino acid substitution in Candida albicans sterol 14alpha-demethylase causes fluconazole resistance in the clinic through reduced affinity","volume":"262","author":"Kelly S.L.","year":"1999","unstructured":"Kelly S.L., , Lamb D.C., , Loeffler J., , Einsele H., , Kelly D.E. 1999. The G464S amino acid substitution in Candida albicans sterol 14alpha-demethylase causes fluconazole resistance in the clinic through reduced affinity. Biochem. Biophys. Res. Commun., 262:174\u2013179.","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"e_1_3_3_17_2","doi-asserted-by":"crossref","first-page":"5682","DOI":"10.1074\/jbc.272.9.5682","article-title":"The mutation T315A in Candida albicans sterol 14alpha-demethylase causes reduced enzyme activity and fluconazole resistance through reduced affinity","volume":"272","author":"Lamb D.C.","year":"1997","unstructured":"Lamb D.C., , Kelly D.E., , Schunck W.H., , Shyadehi A.Z., , Akhtar M., , Lowe D.J., , Baldwin B.C., , Kelly S.L. 1997. The mutation T315A in Candida albicans sterol 14alpha-demethylase causes reduced enzyme activity and fluconazole resistance through reduced affinity. J. Biol. Chem., 272:5682\u20135688.","journal-title":"J. Biol. Chem."},{"key":"e_1_3_3_18_2","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1128\/AAC.44.1.63-67.2000","article-title":"The R467K amino acid substitution in Candida albicans sterol 14alpha-demethylase causes drug resistance through reduced affinity","volume":"44","author":"Lamb D.C.","year":"2000","unstructured":"Lamb D.C., , Kelly D.E., , White T.C., , Kelly S.L. 2000. The R467K amino acid substitution in Candida albicans sterol 14alpha-demethylase causes drug resistance through reduced affinity. Antimicrob. Agents Chemother., 44:63\u201367.","journal-title":"Antimicrob. Agents Chemother."},{"key":"e_1_3_3_19_2","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/S0378-1097(97)00172-9","article-title":"Molecular analysis of cyp51 from fluconazole-resistant Candida albicans strains","volume":"151","author":"Loffler J.","year":"1997","unstructured":"Loffler J., , Kelly S.L., , Hebart H., , Schumache U., , Lass-Florl C., , Einsele H. 1997. Molecular analysis of cyp51 from fluconazole-resistant Candida albicans strains. FEMS Microbiol. Lett., 151:263\u2013268.","journal-title":"FEMS Microbiol. Lett."},{"issue":"10","key":"e_1_3_3_20_2","doi-asserted-by":"crossref","first-page":"2701","DOI":"10.1099\/00221287-145-10-2701","article-title":"Contribution of mutations in the cytochrome P450 14alpha-demethylase (Erg11p, Cyp51p) to azole resistance in Candida albicans","volume":"145","author":"Marichal P.","year":"1999","unstructured":"Marichal P., , Koymans L., , Willemsens S., , Bellens D., , Verhasselt P., , Luyten W., , Borgers M., , Ramaekers F.C., , Odds F.C., , Bossche H.V. 1999. Contribution of mutations in the cytochrome P450 14alpha-demethylase (Erg11p, Cyp51p) to azole resistance in Candida albicans. Microbiology, 145,Pt 10:2701\u20132713.","journal-title":"Microbiology"},{"key":"e_1_3_3_21_2","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1016\/j.diagmicrobio.2009.11.006","article-title":"Screening for amino acid substitutions in the Candida albicans Erg11 protein of azole-susceptible and azole-resistant clinical isolates: new substitutions and a review of the literature","volume":"66","author":"Morio F.","year":"2010","unstructured":"Morio F., , Loge C., , Besse B., , Hennequin C., , Le Pape P. 2010. Screening for amino acid substitutions in the Candida albicans Erg11 protein of azole-susceptible and azole-resistant clinical isolates: new substitutions and a review of the literature. Diagn. Microbiol. Infect. Dis., 66:373\u2013384.","journal-title":"Diagn. Microbiol. Infect. 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A."},{"key":"e_1_3_3_24_2","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/S0162-0134(01)00388-9","article-title":"Substrate recognition sites in 14alpha-sterol demethylase from comparative analysis of amino acid sequences and X-ray structure of Mycobacterium tuberculosis CYP51","volume":"87","author":"Podust L.M.","year":"2001","unstructured":"Podust L.M., , Stojan J., , Poulos T.L., , Waterman M.R. 2001. Substrate recognition sites in 14alpha-sterol demethylase from comparative analysis of amino acid sequences and X-ray structure of Mycobacterium tuberculosis CYP51. J. Inorg. Biochem., 87:227\u2013235.","journal-title":"J. Inorg. Biochem."},{"key":"e_1_3_3_25_2","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1007\/BF00352101","article-title":"Molecular cloning and characterization of a novel gene of Candida albicans, CDR1, conferring multiple resistance to drugs and antifungals","volume":"27","author":"Prasad R.","year":"1995","unstructured":"Prasad R., , De Wergifosse P., , Goffeau A., , Balzi E. 1995. Molecular cloning and characterization of a novel gene of Candida albicans, CDR1, conferring multiple resistance to drugs and antifungals. Curr. Genet., 27:320\u2013329.","journal-title":"Curr. Genet."},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.1.1"},{"key":"e_1_3_3_27_2","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1128\/AAC.42.2.241","article-title":"Amino acid substitutions in the cytochrome P-450 lanosterol 14alpha-demethylase (CYP51A1) from azole-resistant Candida albicans clinical isolates contribute to resistance to azole antifungal agents","volume":"42","author":"Sanglard D.","year":"1998","unstructured":"Sanglard D., , Ischer F., , Koymans L., , Bille J. 1998. Amino acid substitutions in the cytochrome P-450 lanosterol 14alpha-demethylase (CYP51A1) from azole-resistant Candida albicans clinical isolates contribute to resistance to azole antifungal agents. Antimicrob. Agents Chemother., 42:241\u2013253.","journal-title":"Antimicrob. 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Mycoses, 41,Suppl 1:32\u201338.","journal-title":"Mycoses"},{"key":"e_1_3_3_31_2","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1186\/1471-2180-9-167","article-title":"Rapid detection of ERG11 gene mutations in clinical Candida albicans isolates with reduced susceptibility to fluconazole by rolling circle amplification and DNA sequencing","volume":"9","author":"Wang H.","year":"2009","unstructured":"Wang H., , Kong F., , Sorrell T.C., , Wang B., , McNicholas P., , Pantarat N., , Ellis D., , Xiao M., , Widmer F., , Chen S.C. 2009. Rapid detection of ERG11 gene mutations in clinical Candida albicans isolates with reduced susceptibility to fluconazole by rolling circle amplification and DNA sequencing. BMC Microbiol., 9:167.","journal-title":"BMC Microbiol."},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.6.1704-1713.2002"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.2.568-574.2004"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkn015"}],"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/mdr.2012.0204","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2012.0204","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2012.0204","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:27:31Z","timestamp":1777667251000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/mdr.2012.0204"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,8]]},"references-count":33,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2013,8]]}},"alternative-id":["10.1089\/mdr.2012.0204"],"URL":"https:\/\/doi.org\/10.1089\/mdr.2012.0204","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,8]]}}}