{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T16:41:00Z","timestamp":1790440860520,"version":"4.1.0"},"reference-count":23,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2013,8,1]],"date-time":"2013-08-01T00:00:00Z","timestamp":1375315200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:p>\n                    <jats:bold>\n                      <jats:italic toggle=\"yes\">Aims:<\/jats:italic>\n                    <\/jats:bold>\n                    The purpose of the present study was to investigate the diversity of the genes encoding aminoglycoside-modifying enzymes (AMEs) and their associations with resistance phenotypes and clonality in\n                    <jats:italic toggle=\"yes\">Acinetobacter baumannii<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">Pseudomonas aeruginosa<\/jats:italic>\n                    isolates.\n                    <jats:bold>\n                      <jats:italic toggle=\"yes\">Methods:<\/jats:italic>\n                    <\/jats:bold>\n                    Seventy six\n                    <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                    and 75\n                    <jats:italic toggle=\"yes\">A. baumannii<\/jats:italic>\n                    isolates were collected from three University affiliated hospitals in Tehran. MIC determination of amikacin and gentamicin as well as the disk diffusion method for tobramycin, netilmicin, and kanamycin were carried out. Nine AMEs genes and three RNA methylases were investigated in all isolates using the PCR method. Clonality for\n                    <jats:italic toggle=\"yes\">A. baumannii<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                    was investigated using repetitive extragenic palindromic and enterobacterial repetitive intergenic consensus PCR, respectively.\n                    <jats:bold>\n                      <jats:italic toggle=\"yes\">Results:<\/jats:italic>\n                    <\/jats:bold>\n                    <jats:italic toggle=\"yes\">aph(3\u2032)-VIa<\/jats:italic>\n                    (90.6%) and\n                    <jats:italic toggle=\"yes\">aph(3\u2032)-IIb<\/jats:italic>\n                    (61.8%) were the most prevalent AME genes in\n                    <jats:italic toggle=\"yes\">A. baumannii<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                    , respectively. Eight (26%) amikacin highly resistant\n                    <jats:italic toggle=\"yes\">A. baumannii<\/jats:italic>\n                    isolates were positive for\n                    <jats:italic toggle=\"yes\">armA<\/jats:italic>\n                    methylase. Phenotypes and clonality did not link to the genetic determinants of resistance to aminoglycosides in our isolates.\n                    <jats:bold>\n                      <jats:italic toggle=\"yes\">Conclusions:<\/jats:italic>\n                    <\/jats:bold>\n                    AMEs genes are disseminated in different clones of\n                    <jats:italic toggle=\"yes\">A. baumannii<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                    isolates in Iran. Other than AMEs, there are more complex and multifactorial mechanisms that result in aminoglycoside-resistant phenotypes.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2012.0223","type":"journal-article","created":{"date-parts":[[2013,4,11]],"date-time":"2013-04-11T09:22:44Z","timestamp":1365672164000},"page":"282-288","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":48,"title":["Dissemination of Aminoglycoside-Modifying Enzymes and 16S rRNA Methylases Among\n                    <i>Acinetobacter baumannii<\/i>\n                    and\n                    <i>Pseudomonas aeruginosa<\/i>\n                    Isolates"],"prefix":"10.1177","volume":"19","author":[{"given":"Mohammad","family":"Aghazadeh","sequence":"first","affiliation":[{"name":"Tabriz University of Medical Sciences","place":["Iran"]},{"name":"Tabriz University of Medical Sciences","place":["Iran"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Ahangarzadeh","family":"Rezaee","sequence":"additional","affiliation":[{"name":"Tabriz University of Medical Sciences","place":["Iran"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Reza","family":"Nahaei","sequence":"additional","affiliation":[{"name":"Tabriz University of Medical Sciences","place":["Iran"]},{"name":"Tabriz University of Medical Sciences","place":["Iran"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reza","family":"Mahdian","sequence":"additional","affiliation":[{"name":"Pasteur Institute of Iran","place":["Iran"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omid","family":"Pajand","sequence":"additional","affiliation":[{"name":"Tabriz University of Medical Sciences","place":["Iran"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fereshteh","family":"Saffari","sequence":"additional","affiliation":[{"name":"Tabriz University of Medical Sciences","place":["Iran"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryam","family":"Hassan","sequence":"additional","affiliation":[{"name":"Zanjan University of Medical Sciences","place":["Iran"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zoya","family":"Hojabri","sequence":"additional","affiliation":[{"name":"Tabriz University of Medical Sciences","place":["Iran"]},{"name":"Tabriz University of Medical Sciences","place":["Iran"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2013,8]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00570-08"},{"key":"e_1_3_3_3_2","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1556\/amicr.58.2011.4.11","article-title":"Diversity of aminoglycoside modifying enzyme genes among multidrug resistant Acinetobacrter baumannii genotypes isolated from nosocomial infections in Tehran hospitals and their association with class 1 integrons","volume":"58","author":"Asadollahi K.","year":"2011","unstructured":"Asadollahi K., , Taherikalani M., , Maleki A., , Alizadeh E., , Valadbaigi H., , Soroush S., , Maleki H., , Asadollahi P., , Emaneini M. 2011. Diversity of aminoglycoside modifying enzyme genes among multidrug resistant Acinetobacrter baumannii genotypes isolated from nosocomial infections in Tehran hospitals and their association with class 1 integrons. Acta Microbiol. Immunol., 58:359\u2013370.","journal-title":"Acta Microbiol. Immunol."},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-0691.2000.00181.x"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2009.02.010"},{"key":"e_1_3_3_6_2","unstructured":"Clinical Laboratory Standards Institute. 2011. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-First Informational Supplement. 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