{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:24:36Z","timestamp":1777922676101,"version":"3.51.4"},"reference-count":19,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2013,10,1]],"date-time":"2013-10-01T00:00:00Z","timestamp":1380585600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2013,10]]},"abstract":"\n The prevalence and genetic characteristics of\n Escherichia coli<\/jats:italic>\n and\n Klebsiella pneumoniae<\/jats:italic>\n clinical isolates producing extended-spectrum \u03b2-lactamase (ESBL) were examined. Between October 2010 and March 2011,\n E. coli<\/jats:italic>\n (\n n<\/jats:italic>\n =460) and\n K. pneumoniae<\/jats:italic>\n (\n n<\/jats:italic>\n =78) isolates were collected at a tertiary care hospital in Guadalajara, Mexico. The minimum inhibitory concentration (MIC) for each isolate was determined using a broth microdilution method, and ESBL production was assayed. The presence of \u03b2-lactamase genes,\n bla<\/jats:italic>\n SHV<\/jats:sub>\n ,\n bla<\/jats:italic>\n CTX-M<\/jats:sub>\n , and\n bla<\/jats:italic>\n TLA-1<\/jats:sub>\n , was detected by PCR and confirmed with sequencing. Only ESBL-producing isolates were further subjected to pulsed-field gel electrophoresis (PFGE) and plasmid profiling. All of the ESBL isolates were multidrug resistant and 75\/460 (16.3%)\n E. coli<\/jats:italic>\n isolates and 21\/78 (26.9%)\n K. pneumoniae<\/jats:italic>\n isolates were found to produce ESBL. For the\n E. coli<\/jats:italic>\n isolates, >95% susceptibility to amikacin, meropenem, fosfomycin, imipenem, and nitrofurantoin was observed. For\n K. pneumoniae<\/jats:italic>\n , similar results were obtained, with discrepancies observed for gentamicin and nitrofurantoin. PFGE further identified eleven pulsotypes for\n E. coli<\/jats:italic>\n and three clusters of\n K. pneumoniae<\/jats:italic>\n . CTX-M-15 was detected in 85% of ESBL-producing\n E. coli<\/jats:italic>\n and in 76% of ESBL-producing\n K. pneumoniae<\/jats:italic>\n . In contrast, SHV-5 ESBL was identified in 17% of\n E. coli<\/jats:italic>\n isolates and in 86% of\n K. pneumoniae<\/jats:italic>\n isolates. The\n bla-<\/jats:italic>\n TLA-1<\/jats:sub>\n gene was not detected in any of the 96 isolates analyzed. Overall, CTX-M-15 and SHV-5 were found to have a high rate of spread throughout the hospital and were associated with strong multidrug resistance.\n <\/jats:p>","DOI":"10.1089\/mdr.2012.0263","type":"journal-article","created":{"date-parts":[[2013,6,1]],"date-time":"2013-06-01T10:45:12Z","timestamp":1370083512000},"page":"378-383","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":21,"title":["Characterization of\n Enterobacteriaceae<\/i>\n Isolates Obtained from a Tertiary Care Hospital in Mexico, Which Produces Extended-Spectrum \u03b2-Lactamase"],"prefix":"10.1177","volume":"19","author":[{"given":"Rayo","family":"Morf\u00edn-Otero","sequence":"first","affiliation":[{"name":"Universidad de Guadalajara","place":["Mexico"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soraya","family":"Mendoza-Olazar\u00e1n","sequence":"additional","affiliation":[{"name":"Universidad Aut\u00f3noma de Nuevo Le\u00f3n","place":["Mexico"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jes\u00fas","family":"Silva-S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Salud P\u00fablica (CISEI)","place":["Mexico"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduardo","family":"Rodr\u00edguez-Noriega","sequence":"additional","affiliation":[{"name":"Universidad de Guadalajara","place":["Mexico"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge","family":"Laca-D\u00edaz","sequence":"additional","affiliation":[{"name":"Universidad Aut\u00f3noma de Nuevo Le\u00f3n","place":["Mexico"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Perla","family":"Tinoco-Carrillo","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Salud P\u00fablica (CISEI)","place":["Mexico"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis","family":"Petersen","sequence":"additional","affiliation":[{"name":"Universidad de Guadalajara","place":["Mexico"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Perla","family":"L\u00f3pez","sequence":"additional","affiliation":[{"name":"Universidad Aut\u00f3noma de Nuevo Le\u00f3n","place":["Mexico"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando","family":"Reyna-Flores","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Salud P\u00fablica (CISEI)","place":["Mexico"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dolores","family":"Alcantar-Curiel","sequence":"additional","affiliation":[{"name":"Universidad Nacional Aut\u00f3noma de M\u00e9xico","place":["Mexico"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulises","family":"Garza-Ramos","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Salud P\u00fablica (CISEI)","place":["Mexico"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elvira","family":"Garza-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Universidad Aut\u00f3noma de Nuevo Le\u00f3n","place":["Mexico"]},{"name":"Universidad Aut\u00f3noma de Nuevo Le\u00f3n","place":["Mexico"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2013,10]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"crossref","first-page":"1067","DOI":"10.1086\/382354","article-title":"Nosocomial bacteremia and urinary tract infections caused by extended-spectrum beta-lactamase-producing Klebsiella pneumoniae with plasmids carrying both SHV-5 and TLA-1 genes","volume":"38","author":"Alcantar-Curiel D.","year":"2004","unstructured":"Alcantar-Curiel D., , Tinoco J.C., , Gayosso C., , Carlos A., , Daza C., , Perez-Prado M.C., , Salcido L., , Santos J.I., , Alpuche-Aranda C.M. 2004. Nosocomial bacteremia and urinary tract infections caused by extended-spectrum beta-lactamase-producing Klebsiella pneumoniae with plasmids carrying both SHV-5 and TLA-1 genes. Clin Infect Dis, 38:1067\u20131074.","journal-title":"Clin Infect Dis"},{"key":"e_1_3_3_3_2","doi-asserted-by":"crossref","first-page":"4032","DOI":"10.1128\/AAC.05980-11","article-title":"A plasmid-encoded class 1 integron contains GES-type extended-spectrum beta-lactamases in Enterobacteriaceae clinical isolates in Mexico","volume":"56","author":"Barrios H.","year":"2012","unstructured":"Barrios H., , Garza-Ramos U., , Ochoa-Sanchez L.E., , Reyna-Flores F., , Rojas-Moreno T., , Morfin-Otero R., , Rodriguez-Noriega E., , Garza-Gonzalez E., , Gonzalez G., , Volkow P. et al. 2012. A plasmid-encoded class 1 integron contains GES-type extended-spectrum beta-lactamases in Enterobacteriaceae clinical isolates in Mexico. Antimicrob Agents Chemother, 56:4032\u20134034.","journal-title":"Antimicrob Agents Chemother"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.1.1-14.2004"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.4.933-951.2001"},{"key":"e_1_3_3_6_2","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1099\/jmm.0.022970-0","article-title":"Molecular characterization and antimicrobial susceptibility of extended-spectrum {beta}-lactamase-producing Enterobacteriaceae isolates at a tertiary-care centre in Monterrey, Mexico","volume":"60","author":"Garza-Gonzalez E.","year":"2011","unstructured":"Garza-Gonzalez E., , Mendoza Ibarra S.I., , Llaca-Diaz J.M., , Gonzalez G.M. 2011. Molecular characterization and antimicrobial susceptibility of extended-spectrum {beta}-lactamase-producing Enterobacteriaceae isolates at a tertiary-care centre in Monterrey, Mexico. J Med Microbiol, 60:84\u201390.","journal-title":"J Med Microbiol"},{"key":"e_1_3_3_7_2","first-page":"M100","volume-title":"Twenty-Second Informational Supplement","author":"Institute C.A.L.S","year":"2012","unstructured":"Institute C.A.L.S. 2012. Performance Standards for Antimicrobial Susceptibility Testing. Twenty-Second Informational Supplement. C.A.L.S: Wayne, PA, M100\u2013S22."},{"key":"e_1_3_3_8_2","doi-asserted-by":"crossref","first-page":"2364","DOI":"10.1128\/AAC.05824-11","article-title":"Molecular epidemiological analysis of Escherichia coli sequence type ST131 (O25:H4) and blaCTX-M-15 among extended-spectrum-beta-lactamase-producing E. coli from the United States, 2000 to 2009","volume":"56","author":"Johnson J.R.","year":"2012","unstructured":"Johnson J.R., , Urban C., , Weissman S.J., , Jorgensen J.H., , Lewis J.S.2nd, , Hansen G., , Edelstein P.H., , Robicsek A., , Cleary T., , Adachi J. et al. 2012. Molecular epidemiological analysis of Escherichia coli sequence type ST131 (O25:H4) and blaCTX-M-15 among extended-spectrum-beta-lactamase-producing E. coli from the United States, 2000 to 2009. Antimicrob Agents Chemother, 56:2364\u20132370.","journal-title":"Antimicrob Agents Chemother"},{"key":"e_1_3_3_9_2","first-page":"33","article-title":"Pulsed-field gel electrophoresis","volume":"15","author":"Kaufmann M.E.","year":"1998","unstructured":"Kaufmann M.E. 1998. Pulsed-field gel electrophoresis. Methods Mol Med, 15:33\u201350.","journal-title":"Methods Mol Med"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0147-619X(84)90063-5"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00171-08"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2011.03570.x"},{"key":"e_1_3_3_13_2","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1016\/j.ijid.2008.03.008","article-title":"Molecular epidemiology and risk factors of bloodstream infections caused by extended-spectrum beta-lactamase-producing Klebsiella pneumoniae A case-control study","volume":"12","author":"Mosqueda-Gomez J.L.","year":"2008","unstructured":"Mosqueda-Gomez J.L., , Montano-Loza A., , Rolon A.L., , Cervantes C., , Bobadilla-del-Valle J.M., , Silva-Sanchez J., , Garza-Ramos U., , Villasis-Keever A., , Galindo-Fraga A., , Palacios G.M. et al. 2008. Molecular epidemiology and risk factors of bloodstream infections caused by extended-spectrum beta-lactamase-producing Klebsiella pneumoniae A case-control study. Int J Infect Dis, 12:653\u2013659.","journal-title":"Int J Infect Dis"},{"key":"e_1_3_3_14_2","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1128\/JCM.06025-11","article-title":"Molecular epidemiology over an 11-year period (2000 to 2010) of extended-spectrum beta-lactamase-producing Escherichia coli causing bacteremia in a centralized Canadian region","volume":"50","author":"Peirano G.","year":"2012","unstructured":"Peirano G., , van der Bij A.K., , Gregson D.B., , Pitout J.D. 2012. Molecular epidemiology over an 11-year period (2000 to 2010) of extended-spectrum beta-lactamase-producing Escherichia coli causing bacteremia in a centralized Canadian region. 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