{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:24:54Z","timestamp":1777922694219,"version":"3.51.4"},"reference-count":15,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2014,8,1]],"date-time":"2014-08-01T00:00:00Z","timestamp":1406851200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:p>\n                    Twelve nonduplicated KPC-2-producing enterobacterial isolates, including three\n                    <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                    , two\n                    <jats:italic toggle=\"yes\">Citrobacter freundii<\/jats:italic>\n                    , two\n                    <jats:italic toggle=\"yes\">Enterobacter cloacae<\/jats:italic>\n                    , four\n                    <jats:italic toggle=\"yes\">Enterobacter aerogenes<\/jats:italic>\n                    , and one\n                    <jats:italic toggle=\"yes\">Klebsiella oxytoca<\/jats:italic>\n                    , were collected from various clinical samples within 18 months (March 2011 to September 2012). Two of the 12 patients died from infections caused by KPC-2-producing pathogens, while the rest of the patients with KPC-2-producing pathogens improved or were cured. The majority of the clinical isolates exhibited a high-level of resistance to oxyimino-cephalosporins and carbapenems, and possessed self-transferable\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>KPC-2<\/jats:sub>\n                    -carrying plasmids with sizes ranging from 20 to 120\u2009kb. Most isolates carried\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>CTX-M<\/jats:sub>\n                    and plasmid-mediated quinolone resistance genes, while some isolates produced 16S rRNA methylases (ArmA or RmtB). The genetic environment of\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>KPC-2<\/jats:sub>\n                    of most clinical strains was consistent with the genetic structure surrounding\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>KPC-2<\/jats:sub>\n                    on the plasmid pKP048, which contains an integration structure of a Tn\n                    <jats:italic toggle=\"yes\">3<\/jats:italic>\n                    -based transposon and partial Tn\n                    <jats:italic toggle=\"yes\">4401<\/jats:italic>\n                    segment. Inserted fragments (truncated\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>TEM<\/jats:sub>\n                    ) were detected upstream of the\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>KPC-2<\/jats:sub>\n                    gene for two\n                    <jats:italic toggle=\"yes\">E. aerogenes<\/jats:italic>\n                    strains. In conclusion, the enterobacterial isolates exhibited sporadic emergence and did not arise by clonal spread at our hospital. The outcome of infections caused by KPC-producing enterobacterial isolates and their mortality were closely associated with the baseline condition of patients. The spread of\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>KPC-2<\/jats:sub>\n                    gene between different enterobacterial species in China was mainly mediated by horizontal transfer of the Tn\n                    <jats:italic toggle=\"yes\">3<\/jats:italic>\n                    -based transposons and not the\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>KPC-2<\/jats:sub>\n                    -carrying plasmids.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2013.0150","type":"journal-article","created":{"date-parts":[[2014,1,16]],"date-time":"2014-01-16T10:55:13Z","timestamp":1389869713000},"page":"264-269","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":22,"title":["Characterization of KPC-2-Producing\n                    <i>Escherichia coli<\/i>\n                    ,\n                    <i>Citrobacter freundii<\/i>\n                    ,\n                    <i>Enterobacter cloacae<\/i>\n                    ,\n                    <i>Enterobacter aerogenes<\/i>\n                    , and\n                    <i>Klebsiella oxytoca<\/i>\n                    Isolates from a Chinese Hospital"],"prefix":"10.1177","volume":"20","author":[{"given":"Yanping","family":"Luo","sequence":"first","affiliation":[{"name":"Chinese PLA General Hospital","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiyong","family":"Yang","sequence":"additional","affiliation":[{"name":"Chinese PLA General Hospital","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liyan","family":"Ye","sequence":"additional","affiliation":[{"name":"Chinese PLA General Hospital","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Guo","sequence":"additional","affiliation":[{"name":"Chinese PLA General Hospital","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiang","family":"Zhao","sequence":"additional","affiliation":[{"name":"Chinese PLA General Hospital","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rong","family":"Chen","sequence":"additional","affiliation":[{"name":"Chinese PLA General Hospital","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Chen","sequence":"additional","affiliation":[{"name":"Chinese PLA Institute for Disease Control & Prevention","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuelin","family":"Han","sequence":"additional","affiliation":[{"name":"Chinese PLA Institute for Disease Control & Prevention","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingya","family":"Zhao","sequence":"additional","affiliation":[{"name":"Chinese PLA Institute for Disease Control & Prevention","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuguang","family":"Tian","sequence":"additional","affiliation":[{"name":"Chinese PLA Institute for Disease Control & Prevention","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Han","sequence":"additional","affiliation":[{"name":"Chinese PLA Institute for Disease Control & Prevention","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2014,8]]},"reference":[{"key":"e_1_3_4_2_2","unstructured":"[CLSI] Clinical and Laboratory Standards Institute. 2012. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Second Informational Supplement (M100-S22). Clinical and Laboratory Standards Institute Wayne Pennsylvania."},{"key":"e_1_3_4_3_2","doi-asserted-by":"crossref","first-page":"5370","DOI":"10.1128\/AAC.05202-11","article-title":"Functional characterization of Tn4401, a Tn3-based transposon involved in blaKPC gene mobilization","volume":"55","author":"Cuzon G.","year":"2011","unstructured":"Cuzon G., , Naas T., and Nordmann P. 2011. Functional characterization of Tn4401, a Tn3-based transposon involved in blaKPC gene mobilization. Antimicrob. Agents Chemother., 55:5370\u20135373.","journal-title":"Antimicrob. Agents Chemother."},{"key":"e_1_3_4_4_2","doi-asserted-by":"crossref","first-page":"2278","DOI":"10.1128\/AAC.05519-11","article-title":"Emergence of Proteus mirabilis harboring blaKPC-2 and qnrD in a Chinese Hospital","volume":"56","author":"Hu Y.Y.","year":"2012","unstructured":"Hu Y.Y., , Cai J.C., , Zhang R., , Zhou H.W., , Sun Q., and Chen G.X. 2012. Emergence of Proteus mirabilis harboring blaKPC-2 and qnrD in a Chinese Hospital. Antimicrob. Agents Chemother., 56:2278\u20132282.","journal-title":"Antimicrob. Agents Chemother."},{"key":"e_1_3_4_5_2","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1007\/s10096-010-1124-7","article-title":"Novel genetic environment of the plasmid-mediated KPC-3 gene detected in Escherichia coli and Citrobacter freundii isolates from China","volume":"30","author":"Li G.","year":"2011","unstructured":"Li G., , Wei Q., , Wang Y., , Du X., , Zhao Y., and Jiang X. 2011. Novel genetic environment of the plasmid-mediated KPC-3 gene detected in Escherichia coli and Citrobacter freundii isolates from China. Eur. J. Clin. Microbiol. Infect. Dis., 30:575\u2013580.","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"e_1_3_4_6_2","doi-asserted-by":"crossref","first-page":"1257","DOI":"10.1128\/AAC.01451-07","article-title":"Genetic structures at the origin of acquisition of the beta-lactamase bla KPC gene","volume":"52","author":"Naas T.","year":"2008","unstructured":"Naas T., , Cuzon G., , Villegas M.V., , Lartigue M.F., , Quinn J.P., and Nordmann P. 2008. Genetic structures at the origin of acquisition of the beta-lactamase bla KPC gene. Antimicrob. Agents Chemother., 52:1257\u20131263.","journal-title":"Antimicrob. Agents Chemother."},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(09)70054-4"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2010.04.005"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2006.3.59"},{"key":"e_1_3_4_10_2","doi-asserted-by":"crossref","first-page":"4333","DOI":"10.1128\/AAC.00260-09","article-title":"Novel genetic environment of the carbapenem-hydrolyzing beta-lactamase KPC-2 among Enterobacteriaceae in China","volume":"53","author":"Shen P.","year":"2009","unstructured":"Shen P., , Wei Z., , Jiang Y., , Du X., , Ji S., , Yu Y., and Li L. 2009. Novel genetic environment of the carbapenem-hydrolyzing beta-lactamase KPC-2 among Enterobacteriaceae in China. Antimicrob. Agents Chemother., 53:4333\u20134338.","journal-title":"Antimicrob. Agents Chemother."},{"key":"e_1_3_4_11_2","doi-asserted-by":"crossref","first-page":"1585","DOI":"10.1007\/s10096-011-1481-x","article-title":"blaKPC and rmtB on a single plasmid in Enterobacter amnigenus and Klebsiella pneumoniae isolates from the same patient","volume":"31","author":"Sheng J.F.","year":"2012","unstructured":"Sheng J.F., , Li J.J., , Tu S., , Sheng Z.K., , Bi S., , Zhu M.H., , Shen X.M., and Li L.J. 2012. blaKPC and rmtB on a single plasmid in Enterobacter amnigenus and Klebsiella pneumoniae isolates from the same patient. Eur. J. Clin. Microbiol. Infect. Dis., 31:1585\u20131591.","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.05035-11"},{"key":"e_1_3_4_13_2","doi-asserted-by":"crossref","first-page":"E506","DOI":"10.1111\/1469-0691.12035","article-title":"Dissemination and characterization of NDM-1-producing Acinetobacter pittii in an intensive care unit in China","volume":"18","author":"Yang J.","year":"2012","unstructured":"Yang J., , Chen Y., Jia X., Luo Y., , Song Q., , Zhao W., , Wang Y., , Liu H., , Zheng D., , Xia Y., et al. 2012. Dissemination and characterization of NDM-1-producing Acinetobacter pittii in an intensive care unit in China. Clin. Microbiol. Infect., 18:E506\u2013E513.","journal-title":"Clin. Microbiol. Infect."},{"key":"e_1_3_4_14_2","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1093\/jac\/dkl541","article-title":"Plasmid-mediated carbapenem-hydrolysing beta-lactamase KPC-2 in carbapenem-resistant Serratia marcescens isolates from Hangzhou, China","volume":"59","author":"Zhang R.","year":"2007","unstructured":"Zhang R., , Zhou H.W., , Cai J.C., , and Chen G.X. 2007. Plasmid-mediated carbapenem-hydrolysing beta-lactamase KPC-2 in carbapenem-resistant Serratia marcescens isolates from Hangzhou, China. J. Antimicrob. Chemother., 59:574\u2013576.","journal-title":"J. Antimicrob. Chemother."},{"key":"e_1_3_4_15_2","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/j.diagmicrobio.2012.02.007","article-title":"Enterobacteriaceae producing the KPC-2 carbapenemase from hospital sewage","volume":"73","author":"Zhang X.","year":"2012","unstructured":"Zhang X., , Lu X., , and Zong Z. 2012. Enterobacteriaceae producing the KPC-2 carbapenemase from hospital sewage. Diagn. Microbiol. Infect. Dis., 73:204\u2013206.","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2012.0016"}],"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/mdr.2013.0150","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2013.0150","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2013.0150","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:28:04Z","timestamp":1777667284000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/mdr.2013.0150"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,8]]},"references-count":15,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2014,8]]}},"alternative-id":["10.1089\/mdr.2013.0150"],"URL":"https:\/\/doi.org\/10.1089\/mdr.2013.0150","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,8]]}}}