{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:24:54Z","timestamp":1777922694236,"version":"3.51.4"},"reference-count":34,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2014,8,1]],"date-time":"2014-08-01T00:00:00Z","timestamp":1406851200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:p>\n                    The aim of the study was to investigate the molecular epidemiology of 87 third-generation cephalosporin-resistant\n                    <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                    (3GC-R Ec) from bloodstream infections in Denmark from 2009. Sixty-eight of the 87 isolates were extended-spectrum beta-lactamase (ESBL) producers, whereas 17 isolates featured AmpC mutations only (without a coexpressed ESBL enzyme) and 2 isolates were producing CMY-22. The majority (82%) of the ESBL-producing isolates in our study were CTX-M-15 producers and primarily belonged to phylogroup B2 (54.4%) or D (23.5%). Further, one of the two CMY-22-producing isolates belonged to B2, whereas only few of the other AmpCs isolates belonged to B2 and D. Pulsed-field gel electrophoresis revealed that both clonal and nonclonal spread of 3GC-R Ec occurred. ST131 was detected in 50% of ESBL-producing isolates. The remaining ESBL-producing isolates belonged to 17 other sequence types (STs), including several other internationally spreading STs (\n                    <jats:italic toggle=\"yes\">e.g<\/jats:italic>\n                    ., ST10, ST69, and ST405). The majority (93%) of the ESBL-producing isolates and one of the CMY-22-producing isolates were multiresistant. In conclusion, 3GC-R in bacteriaemic\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    in Denmark was mostly due to ESBL production, overexpression of AmpC, and to a lesser extent to plasmid-mediated AmpC. The worldwide disseminated CTX-M-15-ST131 was strongly represented in this collection of Danish, bacteriaemic\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    isolates.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2013.0157","type":"journal-article","created":{"date-parts":[[2014,2,11]],"date-time":"2014-02-11T16:24:59Z","timestamp":1392135899000},"page":"316-324","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":21,"title":["Characterization of Third-Generation Cephalosporin-Resistant\n                    <i>Escherichia coli<\/i>\n                    from Bloodstream Infections in Denmark"],"prefix":"10.1177","volume":"20","author":[{"given":"Frank","family":"Hansen","sequence":"first","affiliation":[{"name":"Statens Serum Institut","place":["Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan S.","family":"Olsen","sequence":"additional","affiliation":[{"name":"Statens Serum Institut","place":["Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ole","family":"Heltberg","sequence":"additional","affiliation":[{"name":"Slagelse Sygehus","place":["Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrik S.","family":"Justesen","sequence":"additional","affiliation":[{"name":"Odense University Hospital","place":["Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Fuglsang-Damgaard","sequence":"additional","affiliation":[{"name":"Aalborg University Hospital","place":["Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jenny D.","family":"Knudsen","sequence":"additional","affiliation":[{"name":"Copenhagen University Hospital","place":["Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anette M.","family":"Hammerum","sequence":"additional","affiliation":[{"name":"Statens Serum Institut","place":["Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2014,8]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1016\/j.mib.2010.09.006","article-title":"Alarming beta-lactamase-mediated resistance in multidrug-resistant Enterobacteriaceae","volume":"13","author":"Bush K","year":"2010","unstructured":"Bush K. 2010. Alarming beta-lactamase-mediated resistance in multidrug-resistant Enterobacteriaceae. Curr. Opin. Microbiol., 13:558\u2013564.","journal-title":"Curr. Opin. Microbiol."},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp194"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.10.4555-4558.2000"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkt291"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1001104"},{"key":"e_1_3_3_7_2","doi-asserted-by":"crossref","unstructured":"Doi Y. Park Y.S. Rivera J.I. Adams-Haduch J.M. Hingwe A. Sordillo E.M. et al. 2013. Community-associated extended-spectrum \u03b2-lactamase-producing Escherichia coli infection in the United States. Clin. Infect. 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