{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:07:19Z","timestamp":1791158839224,"version":"4.1.0"},"reference-count":36,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2015,4,1]],"date-time":"2015-04-01T00:00:00Z","timestamp":1427846400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2015,4]]},"abstract":"\n The objective of the study was to assess the prevalence of plasmid-mediated quinolone resistance (PMQR) genes (\n qnrA<\/jats:italic>\n ,\n qnrB<\/jats:italic>\n ,\n qnrC<\/jats:italic>\n ,\n qnrD<\/jats:italic>\n ,\n qnrS<\/jats:italic>\n ,\n aac(6\u2032)-Ib-cr<\/jats:italic>\n ,\n qepA<\/jats:italic>\n , and\n oqxAB<\/jats:italic>\n ) in a collection of 120 extended-spectrum \u03b2-lactamases (ESBLs)-producing enterobacteria and to characterize them. Overall, PMQR determinants were detected in 72 (60%) isolates (20\n Escherichia coli<\/jats:italic>\n , 32\n Klebsiella pneumoniae,<\/jats:italic>\n and 20\n Enterobacter cloacae<\/jats:italic>\n ). PMQR frequencies were as follows:\n qnr<\/jats:italic>\n genes (25.8%),\n oqxAB<\/jats:italic>\n (21.6%), and\n aac(6\u2032)-Ib-cr<\/jats:italic>\n variant (19.2%). Four qnr alleles were identified as\n qnrB1<\/jats:italic>\n (83.8%),\n qnrB4<\/jats:italic>\n (6.4%),\n qnrB2<\/jats:italic>\n (3.2%), and\n qnrS1<\/jats:italic>\n (6.4%). qnr genes were mainly detected in\n E. cloacae<\/jats:italic>\n (50%),\n aac(6\u2032)-Ib-cr<\/jats:italic>\n in\n E. coli<\/jats:italic>\n (47.5%), and\n oqxAB<\/jats:italic>\n in\n K. pneumoniae<\/jats:italic>\n (65%). Overall, blaCTX-M-15 (90.3%) was the most prevalent\n blaESBL<\/jats:italic>\n type followed by\n \n bla\n SHV-12<\/jats:sub>\n <\/jats:italic>\n (6.4%) and\n \n bla\n SHV-27<\/jats:sub>\n <\/jats:italic>\n (2.7%). Rates of mutations in\n gyrA<\/jats:italic>\n and\n parC<\/jats:italic>\n genes were 75% for\n E. coli<\/jats:italic>\n , 72.8% for\n K. pneumoniae<\/jats:italic>\n , and 50% for\n E. cloacae<\/jats:italic>\n . Isolates with mutations in their quinolone resistance-determining regions exhibited high fluoroquinolones resistance levels compared to those with wild ones. Genetic study of PMQR-harboring isolates revealed a great genomic diversity among each\n Enterobacteriaceae<\/jats:italic>\n species. Our findings indicate the high prevalence of PMQR determinants among ESBL-producing\n Enterobacteriaceae<\/jats:italic>\n isolates from our hospital and their diffusion in various unrelated CTX-M-15-producing clones.\n <\/jats:p>","DOI":"10.1089\/mdr.2014.0053","type":"journal-article","created":{"date-parts":[[2014,9,23]],"date-time":"2014-09-23T10:24:32Z","timestamp":1411467872000},"page":"158-166","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":41,"title":["Prevalence and Characterization of Plasmid-Mediated Quinolone Resistance Genes in Extended-Spectrum \u03b2-Lactamase-Producing\n Enterobacteriaceae<\/i>\n in a Tunisian Hospital"],"prefix":"10.1177","volume":"21","author":[{"given":"Sana","family":"Ferjani","sequence":"first","affiliation":[{"name":"Universit\u00e9 de Tunis El Manar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mabrouka","family":"Saidani","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Tunis El Manar"},{"name":"CHU Charles Nicolle"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faouzi Slim","family":"Amine","sequence":"additional","affiliation":[{"name":"CHU Charles Nicolle"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilhem","family":"Boutiba-Ben Boubaker","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Tunis El Manar"},{"name":"CHU Charles Nicolle"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2015,4]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1054\/drup.2000.0167","article-title":"The impact of antibiotic use on resistance development and persistence","volume":"3","author":"Barbosa T.M.","year":"2000","unstructured":"Barbosa T.M., , and Levy S.B.. 2000. 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