{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T13:45:40Z","timestamp":1787319940549,"version":"build-2736575974"},"reference-count":37,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2014,8,6]],"date-time":"2014-08-06T00:00:00Z","timestamp":1407283200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2015,2,1]]},"abstract":"\n \n Objectives:<\/jats:italic>\n <\/jats:bold>\n We present a new plasmid (pOW16C2) with a novel Tn\n 3<\/jats:italic>\n -like transposon harboring\n bla<\/jats:italic>\n VIM-1<\/jats:sub>\n from a\n Klebsiella pneumoniae<\/jats:italic>\n strain isolated from river water in Switzerland.\n \n Methods:<\/jats:italic>\n <\/jats:bold>\n Complete nucleotide sequence of pOW16C2 was obtained using a Pacific Biosciences SMRT sequencing approach and coding sequences were predicted.\n \n Results:<\/jats:italic>\n <\/jats:bold>\n The 59,228\u2009bp sequence included a typical IncN-like backbone and a mosaic structure with\n bla<\/jats:italic>\n VIM-1<\/jats:sub>\n ,\n aacA4, aphA15<\/jats:italic>\n ,\n aadA1<\/jats:italic>\n ,\n catB2<\/jats:italic>\n ,\n qnrS1, sul1,<\/jats:italic>\n and\n dfrA14<\/jats:italic>\n conferring resistance to carbapenems and other \u03b2-lactam antibiotics, aminoglycosides, chloramphenicol, quinolones, sulfonamides, and trimethoprim, respectively. Most of these resistance genes were inserted in a class 1 integron that was embedded in a novel Tn\n 3<\/jats:italic>\n -like composite transposon.\n \n Conclusions:<\/jats:italic>\n <\/jats:bold>\n IncN plasmids carrying carbapenemases are frequently isolated from\n K. pneumoniae<\/jats:italic>\n strains in clinical settings. The dissemination of\n K. pneumoniae<\/jats:italic>\n harboring\n bla<\/jats:italic>\n VIM-1<\/jats:sub>\n in surface water is a cause for increased concern to public health.\n <\/jats:p>","DOI":"10.1089\/mdr.2014.0055","type":"journal-article","created":{"date-parts":[[2014,8,6]],"date-time":"2014-08-06T14:35:15Z","timestamp":1407335715000},"page":"43-49","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":12,"title":["A Novel Tn\n 3<\/i>\n -Like Composite Transposon Harboring\n bla<\/i>\n VIM-1<\/sub>\n in\n Klebsiella pneumoniae<\/i>\n spp.\n pneumoniae<\/i>\n Isolated from River Water"],"prefix":"10.1177","volume":"21","author":[{"given":"Katrin","family":"Zurfluh","sequence":"first","affiliation":[{"name":"University of Zurich","place":["Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen A.","family":"Power","sequence":"additional","affiliation":[{"name":"College Dublin","place":["Ireland"]},{"name":"University College Dublin","place":["Ireland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jochen","family":"Klumpp","sequence":"additional","affiliation":[{"name":"Institute of Food, Nutrition, and Health","place":["Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Wang","sequence":"additional","affiliation":[{"name":"College Dublin","place":["Ireland"]},{"name":"University College Dublin","place":["Ireland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9amus","family":"Fanning","sequence":"additional","affiliation":[{"name":"College Dublin","place":["Ireland"]},{"name":"University College Dublin","place":["Ireland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger","family":"Stephan","sequence":"additional","affiliation":[{"name":"University of Zurich","place":["Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2014,8,6]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2012.03823.x"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-9-75"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1995.1220"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciu190"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2012.03821.x"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01707-08"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkq269"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2005.03.018"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1207.051555"},{"key":"e_1_3_3_11_2","unstructured":"[CLSI] Clinical and Laboratory Standards Institute. 2011. 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