{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:25:13Z","timestamp":1777922713948,"version":"3.51.4"},"reference-count":57,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2014,9,4]],"date-time":"2014-09-04T00:00:00Z","timestamp":1409788800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2015,2,1]]},"abstract":"<jats:p>\n                    A total of 2,683 nonrepetitive\n                    <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                    isolates were collected from microbiological laboratories covering all regions of the Czech Republic, during April 2011. Antimicrobial susceptibility patterns of\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    were assessed. All 38 cefotaxime-resistant (CTX-R) isolates were found to be extended-spectrum \u03b2-lactamase (ESBL)-positive by the double-disc synergy test. Thirty-two of those isolates produced enzymes of CTX-M-1 family, five of CTX-M-9 family, and one isolate both CTX-M types. Genotyping by multilocus sequence typing classified all ESBL-producing isolates into 13 sequence types (STs). ST131 was the most prevalent and was exclusively correlated with\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    belonging to the more-virulent phylogroup B2.\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>CTX-M-15<\/jats:sub>\n                    and\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>CTX-M-9<\/jats:sub>\n                    -like genes were mainly carried by plasmids belonging to the IncF group, while replicon I1 was predominant among CTX-M-1-encoding plasmids. Additionally, 63% of the ESBL-producing isolates were also resistant to ciprofloxacin. Sequence analysis of quinolone resistance-determining regions of\n                    <jats:italic toggle=\"yes\">gyrA<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">parC<\/jats:italic>\n                    revealed the presence of amino acid substitutions in 22 out of 23 ciprofloxacin-resistant isolates. The\n                    <jats:italic toggle=\"yes\">acc(6\u2032)-Ib-cr<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">qnrB<\/jats:italic>\n                    1 plasmid-mediated quinolone resistance genes were also detected in some of the isolates. This is the first report on the emergence and spread of CTX-M-producing\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    in the community of the Czech Republic, indicating the high prevalence of ST131 clone among CTX-M producers.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2014.0070","type":"journal-article","created":{"date-parts":[[2014,9,4]],"date-time":"2014-09-04T15:20:45Z","timestamp":1409844045000},"page":"74-84","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":14,"title":["High Prevalence of ST131 Among CTX-M-Producing\n                    <i>Escherichia coli<\/i>\n                    from Community-Acquired Infections, in the Czech Republic"],"prefix":"10.1177","volume":"21","author":[{"given":"Costas C.","family":"Papagiannitsis","sequence":"first","affiliation":[{"name":"Charles University in Prague","place":["Czech Republic"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vendula","family":"\u0160tudentov\u00e1","sequence":"additional","affiliation":[{"name":"Charles University in Prague","place":["Czech Republic"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladislav","family":"Jakub\u016f","sequence":"additional","affiliation":[{"name":"National Institute of Public Health","place":["Czech Republic"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petra","family":"\u0160pan\u011blov\u00e1","sequence":"additional","affiliation":[{"name":"National Institute of Public Health","place":["Czech Republic"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pavla","family":"Urb\u00e1\u0161kov\u00e1","sequence":"additional","affiliation":[{"name":"National Institute of Public Health","place":["Czech Republic"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helena","family":"\u017demli\u010dkov\u00e1","sequence":"additional","affiliation":[{"name":"National Institute of Public Health","place":["Czech Republic"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaroslav","family":"Hrab\u00e1k","sequence":"additional","affiliation":[{"name":"Charles University in Prague","place":["Czech Republic"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Czech Participants of European Antimicrobial Resistance Surveillance Network","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2014,9,4]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/1469-0691.12128"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1157\/13119488"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp108"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkf151"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1995.1220"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01647010"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1086\/500936"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1086\/514217"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-12-315"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp122"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.4.933-951.2001"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2005.03.018"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01542-07"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm204"},{"key":"e_1_3_3_16_2","first-page":"868","article-title":"Genomic analysis uncovers a phenotypically diverse but genetically homogeneous Escherichia coli ST131 clone circulating in unrelated urinary tract infections","volume":"67","author":"Clark G.","year":"2012","unstructured":"Clark G., , Paszkiewicz K., , Hale J., , Wenston V., , Constantinidou C., , Penn C., , Achtman M., , and McNally A. 2012. Genomic analysis uncovers a phenotypically diverse but genetically homogeneous Escherichia coli ST131 clone circulating in unrelated urinary tract infections. J. Antimicrob. Agents, 67:868\u2013877.","journal-title":"J. Antimicrob. 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