{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T02:17:55Z","timestamp":1779243475042,"version":"3.51.4"},"reference-count":40,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2014,5,19]],"date-time":"2014-05-19T00:00:00Z","timestamp":1400457600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2014,5,19]],"date-time":"2014-05-19T00:00:00Z","timestamp":1400457600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2014,6,1]]},"abstract":"<jats:p>\n                    <jats:italic toggle=\"yes\">Streptococcus pneumoniae<\/jats:italic>\n                    penicillin-binding protein 2x (PBP2x) is an enzyme involved in the last stages of peptidoglycan assembly and essential for bacterial growth and survival. PBP2x localizes to the division site, a process that depends on its\n                    <jats:underline>P<\/jats:underline>\n                    enicillin-Binding Protein\n                    <jats:underline>A<\/jats:underline>\n                    nd\n                    <jats:underline>S<\/jats:underline>\n                    erine-\n                    <jats:underline>T<\/jats:underline>\n                    hreonine-kinase\n                    <jats:underline>A<\/jats:underline>\n                    ssociated (PASTA) domains, which was previously demonstrated via GFP-PBP2x in living cells. During this study a mutant strain was isolated in which the GFP-PBP2x fusion protein did not localize at division sites and it contained reduced amounts of the full-length GFP-PBP2x. We now show that this defect is due to a point mutation within the C-terminal PASTA2 domain of PBP2x. The mutant protein was analyzed in detail in terms of beta-lactam binding, functionality, and localization in live cells. We demonstrate that the mutation affects the GFP-tagged PBP2x variant severely and renders it susceptible to the protease\/chaperone HtrA.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2014.0082","type":"journal-article","created":{"date-parts":[[2014,5,19]],"date-time":"2014-05-19T14:40:24Z","timestamp":1400510424000},"page":"250-257","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":12,"title":["Penicillin-Binding Protein 2x of\n                    <i>Streptococcus pneumoniae<\/i>\n                    : The Mutation Ala707Asp Within the C-terminal PASTA2 Domain Leads to Destabilization"],"prefix":"10.1177","volume":"20","author":[{"given":"Inga","family":"Schweizer","sequence":"first","affiliation":[{"name":"University of Kaiserslautern","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katharina","family":"Peters","sequence":"additional","affiliation":[{"name":"University of Kaiserslautern","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christoph","family":"Stahlmann","sequence":"additional","affiliation":[{"name":"University of Kaiserslautern","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Regine","family":"Hakenbeck","sequence":"additional","affiliation":[{"name":"University of Kaiserslautern","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dalia","family":"Denapaite","sequence":"additional","affiliation":[{"name":"University of Kaiserslautern","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2014,5,19]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1996.tb02556.x"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00184-13"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/bip.22447"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M107608200"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2009.06872.x"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.66.4.702-738.2002"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2000.3740"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1982.tb06860.x"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.12.2"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2006.00584.x"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(83)80284-8"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.20.6552-6555.1999"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1993.tb05954.x"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-3002(60)90205-5"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12408"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01148-06"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2006.00024.x"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2010.12.016"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.5.1959-1968.2006"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12189"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2007.12.058"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2012.0022"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002182"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03767.x"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12348"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.116.4.491"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.12.3852-3856.1999"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsb0396-284"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12588"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/20.22.6103"},{"key":"e_1_3_3_32_2","unstructured":"Sambrook J. 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