{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T04:32:24Z","timestamp":1786509144155,"version":"build-2736575974"},"reference-count":15,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2015,4,1]],"date-time":"2015-04-01T00:00:00Z","timestamp":1427846400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2015,4]]},"abstract":"\n The most important resistance mechanism against \u03b2-lactam drugs is the production of carbapenemases. In this study, we report the first identification of\n Klebsiella pneumoniae<\/jats:italic>\n carbapenemase (KPC)-2 and New Delhi metallo-\u03b2-lactamase (NDM)-1 in\n Enterobacter hormaechei<\/jats:italic>\n subps.\n oharae<\/jats:italic>\n from Brazil. The detection of carbapenemases was done by phenotypic assays, PCR, and DNA sequencing, whereas the identification was performed by conventional techniques, sequencing of the 16S rDNA gene, and\n hsp60<\/jats:italic>\n -genotyping. Molecular typing was performed using pulsed-field gel electrophoresis, and antimicrobial susceptibility was surrogated by the Etest methodology. Using the whole genome sequencing approach, we searched for resistance genes, plasmid incompatibility group genes, and the genetic environment of\n bla<\/jats:italic>\n NDM<\/jats:sub>\n and\n bla<\/jats:italic>\n KPC<\/jats:sub>\n . The plasmid identification was done by restriction digests with the S1 nuclease followed by hybridization using digoxigenin labeled specific probes. The isolate was considered multiresistant, being susceptible to amikacin and polymyxin B. We observed the following resistance genes:\n bla<\/jats:italic>\n CTX-M-15<\/jats:sub>\n ,\n bla<\/jats:italic>\n ACT-7<\/jats:sub>\n ,\n bla<\/jats:italic>\n TEM-1<\/jats:sub>\n ,\n bla<\/jats:italic>\n OXA-1<\/jats:sub>\n ,\n aadA1<\/jats:italic>\n ,\n aadA2<\/jats:italic>\n ,\n strA<\/jats:italic>\n ,\n strB<\/jats:italic>\n ,\n aac(3)-II<\/jats:italic>\n ,\n qnrB1<\/jats:italic>\n , and\n aac(6\u2032)-Ib-cr<\/jats:italic>\n and incompatibility group plasmid genes IncA\/C, IncHI2, and IncN. The\n bla<\/jats:italic>\n KPC<\/jats:sub>\n gene was found associated to the transposon Tn\n 4401<\/jats:italic>\n isoform b in plasmid with 50 kb (IncN) and\n bla<\/jats:italic>\n NDM-1<\/jats:sub>\n was flanked by a truncated IS\n Aba125<\/jats:italic>\n and\n ble<\/jats:italic>\n MBL<\/jats:sub>\n in plasmid with 160 kb (IncA\/C). This study showed the coproduction of two important carbapenemases (KPC-2 and NDM-1) associated with mobile genetic elements of worldwide epidemiological importance (Tn\n 4401<\/jats:italic>\n and IS\n Aba125<\/jats:italic>\n , respectively), reinforcing the idea that urgent measures are necessary to reduce and prevent the spreading of these carbapenemases primarily in the hospital settings.\n <\/jats:p>","DOI":"10.1089\/mdr.2014.0171","type":"journal-article","created":{"date-parts":[[2014,12,4]],"date-time":"2014-12-04T14:58:56Z","timestamp":1417705136000},"page":"234-236","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":51,"title":["Coproduction of NDM-1 and KPC-2 in\n Enterobacter hormaechei<\/i>\n from Brazil"],"prefix":"10.1177","volume":"21","author":[{"given":"Polyana Silva","family":"Pereira","sequence":"first","affiliation":[{"name":"Instituto Oswaldo Cruz (IOC)","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirla","family":"Borghi","sequence":"additional","affiliation":[{"name":"Instituto Oswaldo Cruz (IOC)","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodolpho Mattos","family":"Albano","sequence":"additional","affiliation":[{"name":"Universidade do Estado do Rio de Janeiro (UERJ)","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan Christian Oliveira","family":"Lopes","sequence":"additional","affiliation":[{"name":"Instituto Oswaldo Cruz (IOC)","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melise Chaves","family":"Silveira","sequence":"additional","affiliation":[{"name":"Instituto Oswaldo Cruz (IOC)","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth Andrade","family":"Marques","sequence":"additional","affiliation":[{"name":"Universidade do Estado do Rio de Janeiro (UERJ)","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane Cleide Ribeiro","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Central de Sa\u00fade P\u00fablica do Rio de Janeiro Noel Nutels (LACEN\/RJ)","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marise Dutra","family":"Asensi","sequence":"additional","affiliation":[{"name":"Instituto Oswaldo Cruz (IOC)","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Paula D'Alincourt","family":"Carvalho-Assef","sequence":"additional","affiliation":[{"name":"Instituto Oswaldo Cruz (IOC)","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2015,4]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1995.1220"},{"key":"e_1_3_4_3_2","doi-asserted-by":"crossref","first-page":"2956","DOI":"10.1093\/jac\/dkt298","article-title":"Isolation of NDM-producing Providencia rettgeri in Brazil","volume":"68","author":"Carvalho-Assef A.P.","year":"2013","unstructured":"Carvalho-Assef A.P., , Pereira P.S., , Albano R.M., , Beri\u00e3o G.C., , Chagas T.P., , Timm L.N., , Da Silva R.C., , Falci D.R., , and Asensi M.D.. 2013. Isolation of NDM-producing Providencia rettgeri in Brazil. J. Antimicrob. Chemother., 68:2956\u20132957.","journal-title":"J. Antimicrob. Chemother."},{"key":"e_1_3_4_4_2","doi-asserted-by":"crossref","first-page":"2475","DOI":"10.1128\/AAC.02804-13","article-title":"Detection of NDM-1-, CTX-M-15-, and qnrB4-producing Enterobacter hormaechei isolates in Brazil","volume":"58","author":"Carvalho-Assef A.P.","year":"2014","unstructured":"Carvalho-Assef A.P., , Pereira P.S., , Albano R.M., , Beri\u00e3o G.C., , Tavares C.P., , Chagas T.P., , Marques E.A., , Timm L.N., , Da Silva R.C., , Falci D.R., , and Asensi M.D.. 2014. Detection of NDM-1-, CTX-M-15-, and qnrB4-producing Enterobacter hormaechei isolates in Brazil. Antimicrob. Agents Chemother., 58:2475\u20132476.","journal-title":"Antimicrob. Agents Chemother."},{"key":"e_1_3_4_5_2","doi-asserted-by":"crossref","first-page":"5370","DOI":"10.1128\/AAC.05202-11","article-title":"Functional characterization of Tn4401, a Tn3-based transposon involved in blaKPC gene mobilization","volume":"55","author":"Cuzon G.","year":"2011","unstructured":"Cuzon G., , Naas T., , and Nordmann P.. 2011. Functional characterization of Tn4401, a Tn3-based transposon involved in blaKPC gene mobilization. Antimicrob. Agents Chemother., 55:5370\u20135373.","journal-title":"Antimicrob. Agents Chemother."},{"key":"e_1_3_4_6_2","doi-asserted-by":"crossref","first-page":"5306","DOI":"10.1128\/AEM.69.9.5306-5318.2003","article-title":"Population genetics of the nomenspecies Enterobacter cloacae","volume":"69","author":"Hoffmann H.","year":"2003","unstructured":"Hoffmann H., , and Roggenkamp A.. 2003. Population genetics of the nomenspecies Enterobacter cloacae. Appl. Environ. Microbiol., 69:5306\u20135318.","journal-title":"Appl. Environ. 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Agents Chemother."},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1710.110655"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01773-10"},{"key":"e_1_3_4_11_2","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1093\/jac\/dks396","article-title":"Update of the molecular epidemiology of KPC-2-producing Klebsiella pneumoniae in Brazil: spread of clonal complex 11 (ST11, ST437 and ST340)","volume":"68","author":"Pereira P.S","year":"2013","unstructured":"Pereira P.S, , de Araujo C.F., , Seki L.M., , Zahner V., , Carvalho-Assef A.P., , and Asensi M.D. 2013. Update of the molecular epidemiology of KPC-2-producing Klebsiella pneumoniae in Brazil: spread of clonal complex 11 (ST11, ST437 and ST340). J. Antimicrob. Chemother., 68:312\u2013316.","journal-title":"J. Antimicrob. Chemother."},{"key":"e_1_3_4_12_2","doi-asserted-by":"crossref","first-page":"2958","DOI":"10.1128\/AAC.02341-13","article-title":"Complete nucleotide sequences of two bla\n KPC-2-bearing IncN plasmids isolated from sequence type 442 Klebsiella pneumoniae clinical strains four years apart","volume":"58","author":"P\u00e9rez-Chaparro P.J.","year":"2014","unstructured":"P\u00e9rez-Chaparro P.J., , Cerdeira L.T., , Queiroz M.G., , de Lima C.P., , Levy C.E., , Pavez M., , Lincopan N., , Gon\u00e7alves E.C., , Mamizuka E.M., , Sampaio J.L., , Nunes M.R., , and McCulloch J.A.. 2014. Complete nucleotide sequences of two blaKPC-2-bearing IncN plasmids isolated from sequence type 442 Klebsiella pneumoniae clinical strains four years apart. Antimicrob. Agents Chemother., 58:2958\u20132960.","journal-title":"Antimicrob. Agents Chemother."},{"key":"e_1_3_4_13_2","doi-asserted-by":"crossref","first-page":"1087","DOI":"10.1128\/AAC.05620-11","article-title":"Tn125-related acquisition of bla\n NDM-like genes in Acinetobacter baumannii","volume":"56","author":"Poirel L.","year":"2012","unstructured":"Poirel L., , Bonnin R.A., , Boulanger A., , Schrenzel J., , Kaase M., , and Nordmann P.. 2012. Tn125-related acquisition of blaNDM-like genes in Acinetobacter baumannii. Antimicrob. Agents Chemother., 56:1087\u20131089.","journal-title":"Antimicrob. 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