{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:25:24Z","timestamp":1777922724943,"version":"3.51.4"},"reference-count":23,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2015,4,1]],"date-time":"2015-04-01T00:00:00Z","timestamp":1427846400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:p>\n                    Serogroup 6 of\n                    <jats:italic toggle=\"yes\">Streptococcus pneumoniae<\/jats:italic>\n                    contains four established serotypes (6A\u20136D). Recently, putative serotype 6E (genotype 6E) was proposed as a novel type, which is cross-reactive with 6B-specific antiserum, but its capsular polysaccharide synthesis (\n                    <jats:italic toggle=\"yes\">cps<\/jats:italic>\n                    ) locus is genetically distinct from those of serotypes 6A and 6B. In the present study, prevalence of genotype 6E was analyzed by a newly designed multiplex polymerase chain reaction (PCR) for noninvasive or colonizing\n                    <jats:italic toggle=\"yes\">S. pneumoniae<\/jats:italic>\n                    isolates in northern Japan assigned to serogroup 6 in our previous study by the sequential multiplex PCR developed by Pai\n                    <jats:italic toggle=\"yes\">et al.<\/jats:italic>\n                    Among the isolates previously assigned to 6A and 6B, 2.2% (1\/45) and 77.3% (140\/181) of isolates, respectively, were revealed to have\n                    <jats:italic toggle=\"yes\">cps<\/jats:italic>\n                    genes of genotype 6E. Eight 6E isolates selected for further analysis were found to have identical or highly similar sequences of\n                    <jats:italic toggle=\"yes\">cps<\/jats:italic>\n                    genes (\n                    <jats:italic toggle=\"yes\">wzg<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">wzh<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">wze<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">wciN<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">wciP<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">wzy<\/jats:italic>\n                    ) to those of strains previously reported as putative serotype 6E, and all the isolates were classified into sequence type 90 (ST90). Reanalysis of genetic traits on penicillin and macrolide resistance clarified significantly higher rates of three\n                    <jats:italic toggle=\"yes\">pbp<\/jats:italic>\n                    mutations (gPRSP) and\n                    <jats:italic toggle=\"yes\">ermB<\/jats:italic>\n                    in genotype 6E than in serotypes 6A and 6B. These findings suggested a need for detection of genotype 6E in the surveillance of\n                    <jats:italic toggle=\"yes\">S. pneumoniae<\/jats:italic>\n                    serotypes.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2014.0181","type":"journal-article","created":{"date-parts":[[2014,10,31]],"date-time":"2014-10-31T16:23:05Z","timestamp":1414772585000},"page":"209-214","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":17,"title":["High Prevalence of Genotype 6E (Putative Serotype 6E) Among Noninvasive\/Colonization Isolates of\n                    <i>Streptococcus pneumoniae<\/i>\n                    in Northern Japan"],"prefix":"10.1177","volume":"21","author":[{"given":"Mitsuyo","family":"Kawaguchiya","sequence":"first","affiliation":[{"name":"Sapporo Medical University","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noriko","family":"Urushibara","sequence":"additional","affiliation":[{"name":"Sapporo Medical University","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobumichi","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Sapporo Medical University","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2015,4]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"crossref","first-page":"2096","DOI":"10.1128\/JCM.00228-14","article-title":"Prevalence of isolates of Streptococcus pneumoniae putative serotype 6E in South Korea","volume":"52","author":"Baek J.Y.","year":"2014","unstructured":"Baek J.Y., , Park I.H., , Song J.H., , and Ko K.S.. 2014. Prevalence of isolates of Streptococcus pneumoniae putative serotype 6E in South Korea. J. Clin. Microbiol., 52:2096\u20132099.","journal-title":"J. Clin. Microbiol."},{"key":"e_1_3_3_3_2","doi-asserted-by":"crossref","first-page":"e31","DOI":"10.1371\/journal.pgen.0020031","article-title":"Genetic analysis of the capsular biosynthetic locus from all 90 pneumococcal serotypes","volume":"2","author":"Bentley S.D.","year":"2006","unstructured":"Bentley S.D., , Aanensen D.M., , Mavroidi A., , Saunders D., , Rabbinowitsch E., , Collins M., , Donohoe K., , Harris D., , Murphy L., , Quail M.A., et al. 2006. Genetic analysis of the capsular biosynthetic locus from all 90 pneumococcal serotypes. PLoS Genet., 2:e31.","journal-title":"PLoS Genet."},{"key":"e_1_3_3_4_2","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1099\/mic.0.043901-0","article-title":"Evolution of the capsular gene locus of Streptococcus pneumoniae serogroup 6","volume":"157","author":"Bratcher P.E.","year":"2011","unstructured":"Bratcher P.E., , Park I.H., , Oliver M.B., , Hortal M., , Camilli R., , Hollingshead S.K., , Camou T., , and Nahm M.H.. 2011. Evolution of the capsular gene locus of Streptococcus pneumoniae serogroup 6. Microbiology, 157:189\u2013198.","journal-title":"Microbiology"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2012.0180"},{"key":"e_1_3_3_6_2","doi-asserted-by":"crossref","first-page":"1132","DOI":"10.3201\/eid2007.131485","article-title":"Changes in capsule and drug resistance of pneumococci after introduction of PCV7, Japan, 2010\u20132013","volume":"20","author":"Chiba N.","year":"2014","unstructured":"Chiba N., , Morozumi M., , Shouji M., , Wajima T., , Iwata S., , Ubukata K.; Invasive Pneumococcal Diseases Surveillance Study Group. 2014. Changes in capsule and drug resistance of pneumococci after introduction of PCV7, Japan, 2010\u20132013. Emerg. Infect. Dis., 20:1132\u20131139.","journal-title":"Emerg. Infect. 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Antibiotic-resistant invasive pneumococcal clones in Italy. J. Clin. Microbiol., 45:306\u2013312.","journal-title":"J. Clin. Microbiol."},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2013.0196"},{"key":"e_1_3_3_10_2","doi-asserted-by":"crossref","first-page":"3395","DOI":"10.1128\/JCM.01645-13","article-title":"Capsular gene sequences and genotypes of \u201cserotype 6E\u201d. Streptococcus pneumoniae isolates","volume":"51","author":"Ko K.S.","year":"2013","unstructured":"Ko K.S., , Baek J.Y., , and Song J.H.. 2013. Capsular gene sequences and genotypes of \u201cserotype 6E\u201d. Streptococcus pneumoniae isolates. J. Clin. Microbiol., 51:3395\u20133399.","journal-title":"J. Clin. Microbiol."},{"key":"e_1_3_3_11_2","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1111\/j.1469-0691.2010.03182.x","article-title":"Changes in antimicrobial resistance, serotypes and genotypes in Streptococcus pneumoniae over a 30-year period","volume":"16","author":"Li\u00f1ares J.","year":"2010","unstructured":"Li\u00f1ares J., , Ardanuy C., , Pallares R., , and Fenoll A.. 2010. Changes in antimicrobial resistance, serotypes and genotypes in Streptococcus pneumoniae over a 30-year period. Clin. Microbiol. Infect., 16:402\u2013410.","journal-title":"Clin. Microbiol. Infect."},{"key":"e_1_3_3_12_2","doi-asserted-by":"crossref","first-page":"8181","DOI":"10.1128\/JB.186.24.8181-8192.2004","article-title":"Evolutionary genetics of the capsular locus of serogroup 6 pneumococci","volume":"186","author":"Mavroidi A.","year":"2004","unstructured":"Mavroidi A., , Godoy D., , Aanensen D.M., , Robinson D.A., , Hollingshead S.K., , and Spratt B.G.. 2004. 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