{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T11:59:18Z","timestamp":1789732758814,"version":"4.0.1"},"reference-count":45,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2015,4,17]],"date-time":"2015-04-17T00:00:00Z","timestamp":1429228800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2015,8,1]]},"abstract":"\n Serratia marcescens<\/jats:italic>\n is one of the most important pathogens responsible for nosocomial infections worldwide. Here, we have investigated the molecular support of antibiotic resistance and genetic relationships in a series of 54\n S. marcescens<\/jats:italic>\n clinical isolates collected from Eastern Algeria between December 2011 and July 2013. The 54 isolates were identified by matrix-assisted laser desorption\/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). Antibiotic susceptibility testing was performed by disc diffusion and\n E<\/jats:italic>\n -test methods. Antibiotic resistance genes were detected by polymerase chain reaction (PCR). The genetic transfer of antibiotic resistance was performed by conjugation using azide-resistant\n Escherichia coli J53<\/jats:italic>\n as the recipient strain, and plasmid analysis was done by PCR-based replicon typing. The relatedness of our isolates was determined by phylogenetic analysis based on partial sequences of four protein-encoding genes (\n gyrB<\/jats:italic>\n ,\n rpoB<\/jats:italic>\n ,\n infB<\/jats:italic>\n , and\n atpD<\/jats:italic>\n ) and then compared to MALDI-TOF MS clustering. Thirty-five out of 54 isolates yielded an extended-spectrum \u03b2-lactamase (ESBL) phenotype and carried\n \n bla\n CTX\u2013M-15<\/jats:sub>\n <\/jats:italic>\n (\n n<\/jats:italic>\n =32),\n \n bla\n TEM-1<\/jats:sub>\n <\/jats:italic>\n (\n n<\/jats:italic>\n =26),\n \n bla\n TEM-71<\/jats:sub>\n <\/jats:italic>\n (\n n<\/jats:italic>\n =1),\n \n bla\n SHV-1a<\/jats:sub>\n <\/jats:italic>\n (\n n<\/jats:italic>\n =1), and\n \n bla\n PER-2<\/jats:sub>\n <\/jats:italic>\n (\n n<\/jats:italic>\n =12). Among these isolates, we identified a cluster of 15 isolates from a urology unit that coharbored ESBL and the 16S rRNA methyltransferase\n armA<\/jats:italic>\n . Conjugation was successful for five selected strains, demonstrating the transferability of a conjugative plasmid of incompatibility group incL\/M type. Phylogenetic analysis along with MALDI-TOF clustering likely suggested an outbreak of such isolates in the urology unit. In this study, we report for the first time the co-occurrence of\n armA<\/jats:italic>\n methyltransferase with ESBL in\n S. marcescens<\/jats:italic>\n clinical isolates in Eastern Algeria.\n <\/jats:p>","DOI":"10.1089\/mdr.2014.0240","type":"journal-article","created":{"date-parts":[[2015,4,17]],"date-time":"2015-04-17T18:33:44Z","timestamp":1429295624000},"page":"470-476","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":31,"title":["Outbreak of\n Serratia marcescens<\/i>\n Coproducing ArmA and CTX-M-15 Mediated High Levels of Resistance to Aminoglycoside and Extended-Spectrum Beta-Lactamases, Algeria"],"prefix":"10.1177","volume":"21","author":[{"given":"Rima","family":"Batah","sequence":"first","affiliation":[{"name":"Universit\u00e9 de la M\u00e9diterran\u00e9e","place":["France"]},{"name":"Universit\u00e9 Badji Mokhtar Annaba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lotfi","family":"Loucif","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de la M\u00e9diterran\u00e9e","place":["France"]},{"name":"Universit\u00e9 El Hadj Lakhdar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abiola Olumuyiwa","family":"Olaitan","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de la M\u00e9diterran\u00e9e","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nafissa","family":"Boutefnouchet","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Badji Mokhtar Annaba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamoudi","family":"Allag","sequence":"additional","affiliation":[{"name":"Clinique R\u00e9nale Daksi Constantine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Marc","family":"Rolain","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de la M\u00e9diterran\u00e9e","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2015,4,17]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2013.0193"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0061428"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2008.09.004"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2009.01.010"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2005.03.018"},{"key":"e_1_3_3_7_2","first-page":"373","article-title":"Serratia marcescens in a neonatal intensive care unit: two long-term multiclone outbreaks in a 10-year observational study","volume":"36","author":"Casolari C.","year":"2013","unstructured":"Casolari C., , Pecorari M., , Della C.E., , Cattani S., , Venturelli C., , Fabio G., , Tagliazucchi S., , Serpini G.F., , Migaldi M., , Marchegiano P., , Rumpianesi F., , and Ferrari F. 2013. Serratia marcescens in a neonatal intensive care unit: two long-term multiclone outbreaks in a 10-year observational study. 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