{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T14:21:51Z","timestamp":1783606911444,"version":"3.55.0"},"reference-count":40,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2015,12,1]],"date-time":"2015-12-01T00:00:00Z","timestamp":1448928000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:p>\n                    The mechanism responsible for acquired decreased susceptibility to macrolides (14-membered erythromycin [Ery], 16-membered tylosin [Ty] and tilmicosin [Tm]) and to lincosamides (lincomycin [Ln]) was investigated in\n                    <jats:italic toggle=\"yes\">Mycoplasma synoviae<\/jats:italic>\n                    , a pathogen that causes respiratory infections and synovitis in chicken and turkey. Sequence analysis of domains II and V of the two 23S rRNA alleles and ribosomal proteins L4 and L22 was performed on 49\n                    <jats:italic toggle=\"yes\">M. synoviae<\/jats:italic>\n                    isolates,\n                    <jats:italic toggle=\"yes\">M. synoviae<\/jats:italic>\n                    type strain WVU1853, and reference strain FMT showing minimal inhibitory concentrations (MICs) to Ty (\u22640.015 to 2\u2009\u03bcg\/ml), Tm (0.03 to \u22658\u2009\u03bcg\/ml), and Ln (0.125 to \u22658\u2009\u03bcg\/ml); MICs to Ery ranged from 32 to \u2265128\u2009\u03bcg\/ml. Our results showed that the nucleotide substitution G748A (\n                    <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                    numbering) in domain II of one or both 23S rRNA alleles may account for a slight increase in MICs to Ty and Tm (up to 0.5 and 2\u2009\u03bcg\/ml, respectively). No correlation between the presence of G748A and decreased susceptibility to Ln was found. However, the presence of the point mutations A2058G or A2059G in domain V of one or both alleles of the 23S rRNAs was correlated with a more significant decrease in susceptibility to Ty (1\u20132\u2009\u03bcg\/ml), Tm (\u22658\u2009\u03bcg\/ml), and Ln (\u22658\u2009\u03bcg\/ml). All\n                    <jats:italic toggle=\"yes\">M. synoviae<\/jats:italic>\n                    isolates tested had a G2057A transition in the 23S rRNAs consistent with previously described intrinsic resistance to Ery. Mutations G64E (one isolate) and Q90K\/H (two isolates) were identified in the L4 and L22 proteins, respectively, but their impact on decreased susceptibility to macrolides and lincomycin was not clear.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2014.0290","type":"journal-article","created":{"date-parts":[[2015,3,3]],"date-time":"2015-03-03T11:48:23Z","timestamp":1425383303000},"page":"581-589","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":30,"title":["Decreased Susceptibility to Macrolide\u2013Lincosamide in\n                    <i>Mycoplasma synoviae<\/i>\n                    Is Associated with Mutations in 23S Ribosomal RNA"],"prefix":"10.1177","volume":"21","author":[{"given":"Inna","family":"Lysnyansky","sequence":"first","affiliation":[{"name":"Kimron Veterinary Institute","place":["Israel"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irena","family":"Gerchman","sequence":"additional","affiliation":[{"name":"Kimron Veterinary Institute","place":["Israel"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Flaminio","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale delle Venezie","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salvatore","family":"Catania","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale delle Venezie","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2015,12]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"crossref","first-page":"423","DOI":"10.2217\/fmb.11.18","article-title":"Mycoplasma pneumoniae: susceptibility and resistance to antibiotics","volume":"6","author":"Bebear C.","year":"2011","unstructured":"Bebear C., , Pereyre S., , and Peuchant O. 2011. 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