{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:25:48Z","timestamp":1777922748396,"version":"3.51.4"},"reference-count":28,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2016,3,1]],"date-time":"2016-03-01T00:00:00Z","timestamp":1456790400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2016,3]]},"abstract":"<jats:p>\n                    The complete sequences of two previously reported plasmids carrying plasmid-mediated quinolone resistance genes from\n                    <jats:italic toggle=\"yes\">Shigella flexneri<\/jats:italic>\n                    in China have not been available. The present study using the p5-C3 assembly method revealed that (1) the plasmid pSF07201 with\n                    <jats:italic toggle=\"yes\">aac(6\u2032)-Ib-cr<\/jats:italic>\n                    had 75,335\u2009bp with antibiotic resistance genes\n                    <jats:italic toggle=\"yes\">CTX-M-3<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">TEM-1,<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">FosA3<\/jats:italic>\n                    ; (2) seven fragments of pSF07201 had more than 99% homology with the seven corresponding plasmids; (3) the other plasmid pSF07202 with\n                    <jats:italic toggle=\"yes\">qnrS<\/jats:italic>\n                    had 47,669\u2009bp with antibiotic resistance gene\n                    <jats:italic toggle=\"yes\">TEM-1<\/jats:italic>\n                    and 99.95% homology with a segment of pKF362122, which has the\n                    <jats:italic toggle=\"yes\">qnrS<\/jats:italic>\n                    gene from location 162,490 to 163,146. A conjugation and electrotransformation experiment suggested that these two plasmids might horizontally transfer between and coexist in\n                    <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                    J53 and\n                    <jats:italic toggle=\"yes\">S. flexneri<\/jats:italic>\n                    2a 301. Either the\n                    <jats:italic toggle=\"yes\">aac(6\u2032)-Ib-cr<\/jats:italic>\n                    or\n                    <jats:italic toggle=\"yes\">qnrS<\/jats:italic>\n                    gene contributed to, but only the coexistence of the two genes conferred to the resistance to ciprofloxacin in these two strains. To the best of our knowledge, this is the first report of the complete sequences of the\n                    <jats:italic toggle=\"yes\">aac(6\u2032)-Ib-cr-<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">qnrS<\/jats:italic>\n                    -positive plasmids in\n                    <jats:italic toggle=\"yes\">Shigella<\/jats:italic>\n                    isolates. Our findings indicate that two genes probably evolve through horizontal plasmid transfer between the different bacterial types.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2015.0082","type":"journal-article","created":{"date-parts":[[2015,10,15]],"date-time":"2015-10-15T15:17:02Z","timestamp":1444922222000},"page":"115-122","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["Complete Sequences and Characterization of Two Novel Plasmids Carrying\n                    <i>aac(6\u2032)-Ib-cr<\/i>\n                    and\n                    <i>qnrS<\/i>\n                    Gene in\n                    <i>Shigella flexneri<\/i>"],"prefix":"10.1177","volume":"22","author":[{"given":"Xiao-Ying","family":"Pu","sequence":"first","affiliation":[{"name":"Hangzhou Center for Disease Control and Prevention","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing-Cao","family":"Pan","sequence":"additional","affiliation":[{"name":"Hangzhou Center for Disease Control and Prevention","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ya-Ming","family":"Gu","sequence":"additional","affiliation":[{"name":"Health Bureau of Zhejiang Province","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Zheng","sequence":"additional","affiliation":[{"name":"Hangzhou Center for Disease Control and Prevention","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Li","sequence":"additional","affiliation":[{"name":"Hangzhou Center for Disease Control and Prevention","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hua","family":"Yu","sequence":"additional","affiliation":[{"name":"Hangzhou Center for Disease Control and Prevention","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2016,3]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"crossref","first-page":"515","DOI":"10.1093\/jac\/dkm508","article-title":"Linkage of acquired quinolone resistance (qnrS1) and metallo-beta-lactamase (blaVIM-1) genes in multiple species of Enterobacteriaceae from Bolzano, Italy","volume":"61","author":"Aschbacher R.","year":"2008","unstructured":"Aschbacher R., , Doumith M., , Livermore D.M., , Larcher C., , and Woodford N. 2008. 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