{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:26:01Z","timestamp":1777922761774,"version":"3.51.4"},"reference-count":25,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2016,3,1]],"date-time":"2016-03-01T00:00:00Z","timestamp":1456790400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2016,3]]},"abstract":"\n Bacteria encoding the New Delhi metallo-\u03b2-lactamase gene (\n bla<\/jats:italic>\n NDM-1<\/jats:sub>\n ) are regarded as superbugs for their resistance to multiple antibiotics. Plasmids encoding\n bla<\/jats:italic>\n NDM-1<\/jats:sub>\n have been observed to be spreading among gram-negative bacteria around the world. Previous studies have demonstrated that multiple modifications of\n bla<\/jats:italic>\n NDM-1<\/jats:sub>\n -harboring plasmids might contribute to the spread of the gene. In this study, we analyzed\n bla<\/jats:italic>\n NDM-1<\/jats:sub>\n -encoding plasmids from two\n Klebsiella pneumoniae<\/jats:italic>\n isolates, DU7433 and DU1301, found to be unrelated by pulsed field gel electrophoresis and multilocus sequencing typing (DU7433: ST14 and DU1301: ST11), and compared them with previously published plasmids. Although strains DU1301, DU7433, and previously published strain DU43320 carried unrelated plasmids, their transconjugants exhibited similar antimicrobial resistance profiles. Transconjugants lacked the resistance to aztreonam, ciprofloxacin, gentamicin, tetracycline, and trimethoprim\/sulfamethoxazole when compared with the corresponding clinical isolates. Plasmids pTR1 from DU1301 and pTR2 from DU7433 had completely different plasmid backbones except a short conserved region of\n bla<\/jats:italic>\n NDM-1<\/jats:sub>\n and\n ble<\/jats:italic>\n flanked with truncated or nontruncated IS\n Aba125<\/jats:italic>\n and\n trpF<\/jats:italic>\n . The presence of this common region among known\n bla<\/jats:italic>\n NDM-1<\/jats:sub>\n -carrying plasmids implies that the dissemination of\n bla<\/jats:italic>\n NDM-1<\/jats:sub>\n may be facilitated by mobilization of this conserved immediate region among different plasmids. Control measures should be strictly enforced whenever increasing incidences of epidemiological unrelated strains were identified.\n <\/jats:p>","DOI":"10.1089\/mdr.2015.0132","type":"journal-article","created":{"date-parts":[[2015,8,26]],"date-time":"2015-08-26T11:44:23Z","timestamp":1440589463000},"page":"109-114","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["A Common Flanking Region in Promiscuous Plasmids Encoding\n bla<\/i>\n NDM-1<\/sub>\n in\n Klebsiella pneumoniae<\/i>\n Isolated in Singapore"],"prefix":"10.1177","volume":"22","author":[{"given":"Ying-Tsong","family":"Chen","sequence":"first","affiliation":[{"name":"National Health Research Institutes","place":["Taiwan"]},{"name":"National Chung Hsing University","place":["Taiwan"]},{"name":"National Chung Hsing University","place":["Taiwan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. Kristopher","family":"Siu","sequence":"additional","affiliation":[{"name":"National Health Research Institutes","place":["Taiwan"]},{"name":"China Medical University","place":["Taiwan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu-Kuo","family":"Tsai","sequence":"additional","affiliation":[{"name":"National Health Research Institutes","place":["Taiwan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fu-Mei","family":"Lin","sequence":"additional","affiliation":[{"name":"National Health Research Institutes","place":["Taiwan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tse Hsien","family":"Koh","sequence":"additional","affiliation":[{"name":"Singapore General Hospital","place":["Singapore"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiun-Han","family":"Chen","sequence":"additional","affiliation":[{"name":"Yuanpei University of Medical Technology","place":["Taiwan"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2016,3]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-9-75"},{"key":"e_1_3_3_3_2","doi-asserted-by":"crossref","first-page":"e104899","DOI":"10.1371\/journal.pone.0104899","article-title":"Closely related NDM-1-encoding plasmids from Escherichia coli and Klebsiella pneumoniae in Taiwan","volume":"9","author":"Chen C.J.","year":"2014","unstructured":"Chen C.J., , Wu T.L., , Lu P.L., , Chen Y.T., , Fung C.P., , Chuang Y.C., , Lin J.C., , and Siu L.K. 2014. Closely related NDM-1-encoding plasmids from Escherichia coli and Klebsiella pneumoniae in Taiwan. PLoS One, 9:e104899.","journal-title":"PLoS One"},{"key":"e_1_3_3_4_2","doi-asserted-by":"crossref","first-page":"e48737","DOI":"10.1371\/journal.pone.0048737","article-title":"Sequence of closely related plasmids encoding bla\n NDM-1 in two unrelated Klebsiella pneumoniae isolates in Singapore","volume":"7","author":"Chen Y.T.","year":"2012","unstructured":"Chen Y.T., , Lin A.C., , Siu L.K., , and Koh T.H. 2012. Sequence of closely related plasmids encoding blaNDM-1 in two unrelated Klebsiella pneumoniae isolates in Singapore. PLoS One., 7:e48737.","journal-title":"PLoS One."},{"key":"e_1_3_3_5_2","doi-asserted-by":"crossref","first-page":"3861","DOI":"10.1128\/AAC.00456-06","article-title":"Complete nucleotide sequence of pK245, a 98-kilobase plasmid conferring quinolone resistance and extended-spectrum-beta-lactamase activity in a clinical Klebsiella pneumoniae isolate","volume":"50","author":"Chen Y.T.","year":"2006","unstructured":"Chen Y.T., , Shu H.Y., , Li L.H., , Liao T.L., , Wu K.M., , Shiau Y.R., , Yan J.J., , Su I.J., , Tsai S.F., , and Lauderdale T.L. 2006. Complete nucleotide sequence of pK245, a 98-kilobase plasmid conferring quinolone resistance and extended-spectrum-beta-lactamase activity in a clinical Klebsiella pneumoniae isolate. Antimicrob. Agents Chemother., 50:3861\u20133866.","journal-title":"Antimicrob. Agents Chemother."},{"key":"e_1_3_3_6_2","doi-asserted-by":"crossref","first-page":"1693","DOI":"10.1128\/AAC.05583-11","article-title":"Association of the emerging carbapenemase NDM-1 with a bleomycin resistance protein in Enterobacteriaceae and Acinetobacter baumannii","volume":"56","author":"Dortet L.","year":"2012","unstructured":"Dortet L., , Nordmann P., , and Poirel L. 2012. Association of the emerging carbapenemase NDM-1 with a bleomycin resistance protein in Enterobacteriaceae and Acinetobacter baumannii. Antimicrob. Agents Chemother., 56:1693\u20131697.","journal-title":"Antimicrob. Agents Chemother."},{"key":"e_1_3_3_7_2","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1093\/jac\/dks367","article-title":"Complete nucleotide sequence of the large conjugative pTC2 multireplicon plasmid encoding the VIM-1 metallo-beta-lactamase","volume":"68","author":"Drieux L.","year":"2013","unstructured":"Drieux L., , Decre D., , Frangeul L., , Arlet G., , Jarlier V., , and Sougakoff W. 2013. Complete nucleotide sequence of the large conjugative pTC2 multireplicon plasmid encoding the VIM-1 metallo-beta-lactamase. J. Antimicrob. Chemother., 68:97\u2013100.","journal-title":"J. Antimicrob. 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Agents Chemother."},{"key":"e_1_3_3_9_2","doi-asserted-by":"crossref","first-page":"e17989","DOI":"10.1371\/journal.pone.0017989","article-title":"Complete sequencing of pNDM-HK encoding NDM-1 carbapenemase from a multidrug-resistant Escherichia coli strain isolated in Hong Kong","volume":"6","author":"Ho P.L.","year":"2011","unstructured":"Ho P.L., , Lo W.U., , Yeung M.K., , Lin C.H., , Chow K.H., , Ang I., , Tong A.H., , Bao J.Y., , Lok S., , and Lo J.Y. 2011. Complete sequencing of pNDM-HK encoding NDM-1 carbapenemase from a multidrug-resistant Escherichia coli strain isolated in Hong Kong. PLoS One, 6:e17989.","journal-title":"PLoS One"},{"key":"e_1_3_3_10_2","first-page":"e62774","volume-title":"PLoS One","author":"Huang T.W.","year":"2013","unstructured":"Huang T.W., , Chen T.L., , Chen Y.T., , Lauderdale T.L., , Liao T.L., , Lee Y.T., , Chen C.P., , Liu Y.M., , Lin A.C., , Chang Y.H., et al. 2013. Copy number change of the NDM-1 sequence in a multidrug-resistant Klebsiella pneumoniae clinical isolate. PLoS One, 8:e62774."},{"key":"e_1_3_3_11_2","doi-asserted-by":"crossref","first-page":"3929","DOI":"10.1128\/AAC.00226-11","article-title":"Emergence of NDM-1 metallo-beta-lactamase in Pseudomonas aeruginosa clinical isolates from Serbia","volume":"55","author":"Jovcic B.","year":"2011","unstructured":"Jovcic B., , Lepsanovic Z., , Suljagic V., , Rackov G., , Begovic J., , Topisirovic L., , and Kojic M. 2011. Emergence of NDM-1 metallo-beta-lactamase in Pseudomonas aeruginosa clinical isolates from Serbia. Antimicrob. Agents Chemother., 55:3929\u20133931.","journal-title":"Antimicrob. Agents Chemother."},{"key":"e_1_3_3_12_2","first-page":"600","article-title":"Acquired carbapenemases in Enterobactericeae in Singapore, 1996\u20132012","volume":"45","author":"Koh T.H.","year":"2013","unstructured":"Koh T.H., , Cao D., , Shan Q.Y., , Bacon A., , Hsu L.Y., , and Ooi E.E. 2013. Acquired carbapenemases in Enterobactericeae in Singapore, 1996\u20132012. Pathology, 45:600\u2013603.","journal-title":"Pathology"},{"key":"e_1_3_3_13_2","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1016\/S1473-3099(10)70274-7","article-title":"Global spread of New Delhi metallo-beta-lactamase 1","volume":"10","author":"Koh T.H.","year":"2010","unstructured":"Koh T.H., , Khoo C.T., , Wijaya L., , Leong H.N., , Lo Y.L., , Lim L.C., , and Koh T.Y. 2010. Global spread of New Delhi metallo-beta-lactamase 1. Lancet Infect. Dis., 10:828.","journal-title":"Lancet Infect. 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Agents Chemother."},{"key":"e_1_3_3_23_2","doi-asserted-by":"crossref","first-page":"1645","DOI":"10.1093\/jac\/dks114","article-title":"Complete sequencing of an IncH plasmid carrying the bla\n NDM-1, bla\n CTX-M-15 and qnrB1 genes","volume":"67","author":"Villa L.","year":"2012","unstructured":"Villa L., , Poirel L., , Nordmann P., , Carta C., , and Carattoli A. 2012. Complete sequencing of an IncH plasmid carrying the blaNDM-1, blaCTX-M-15 and qnrB1 genes. J. Antimicrob. Chemother., 67:1645\u20131650.","journal-title":"J. Antimicrob. Chemother."},{"key":"e_1_3_3_24_2","doi-asserted-by":"crossref","first-page":"356","DOI":"10.3390\/pathogens3020356","article-title":"Characterization of antimicrobial resistance dissemination across plasmid communities classified by network analysis","volume":"3","author":"Yamashita A.","year":"2014","unstructured":"Yamashita A., , Sekizuka T., , and Kuroda M. 2014. Characterization of antimicrobial resistance dissemination across plasmid communities classified by network analysis. Pathogens, 3:356\u2013376.","journal-title":"Pathogens"},{"key":"e_1_3_3_25_2","doi-asserted-by":"crossref","first-page":"5046","DOI":"10.1128\/AAC.00774-09","article-title":"Characterization of a new metallo-beta-lactamase gene, bla\n NDM-1, and a novel erythromycin esterase gene carried on a unique genetic structure in Klebsiella pneumoniae sequence type 14 from India","volume":"53","author":"Yong D.","year":"2009","unstructured":"Yong D., , Toleman M.A., , Giske C.G., , Cho H.S., , Sundman K., , Lee K., , and Walsh T.R. 2009. Characterization of a new metallo-beta-lactamase gene, blaNDM-1, and a novel erythromycin esterase gene carried on a unique genetic structure in Klebsiella pneumoniae sequence type 14 from India. Antimicrob. Agents Chemother., 53:5046\u20135054.","journal-title":"Antimicrob. 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