{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T11:51:49Z","timestamp":1790596309743,"version":"4.1.0"},"reference-count":21,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2016,3,14]],"date-time":"2016-03-14T00:00:00Z","timestamp":1457913600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2016,3,14]],"date-time":"2016-03-14T00:00:00Z","timestamp":1457913600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2016,7,1]]},"abstract":"<jats:p>\n                    With the rise in multidrug-resistant (MDR) bacterial infections, there has been increasing interest in combinations of \u22652 antimicrobial agents with synergistic effects. We established an MDR bacterial strain library to screen for\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    antimicrobial synergy by using a broth microdilution checkerboard method and high-throughput luciferase-based bacterial cell viability assay. In total, 39 MDR bacterial strains, including 23 carbapenem-resistant gram-negative bacteria, 9 vancomycin-intermediate\n                    <jats:italic toggle=\"yes\">Staphylococcus aureus<\/jats:italic>\n                    , and 7 vancomycin-resistant\n                    <jats:italic toggle=\"yes\">Enterococcus faecalis<\/jats:italic>\n                    , were used to screen for potential antimicrobial synergies. Synergies were more frequently identified with combinations of imipenem plus trimethoprim\u2013sulfamethoxazole for carbapenem-resistant\n                    <jats:italic toggle=\"yes\">Acinetobacter baumannii<\/jats:italic>\n                    in the library. To verify this finding, we tested 34\n                    <jats:italic toggle=\"yes\">A. baumannii<\/jats:italic>\n                    clinical isolates resistant to both imipenem and trimethoprim\u2013sulfamethoxazole by the checkerboard method. The imipenem plus trimethoprim\u2013sulfamethoxazole combination showed synergy in the treatment of 21 (62%) of the clinical isolates. The results indicate that pilot screening for antimicrobial synergy in the MDR bacterial strain library could be valuable in the selection of combination therapeutic regimens to treat MDR bacterial infections. Further studies are warranted to determine whether this screening system can be useful to screen for the combined effects of conventional antimicrobials and new-generation antimicrobials or nonantimicrobials.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2015.0251","type":"journal-article","created":{"date-parts":[[2016,3,14]],"date-time":"2016-03-14T12:28:20Z","timestamp":1457958500000},"page":"372-378","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":16,"title":["Pilot Screening to Determine Antimicrobial Synergies in a Multidrug-Resistant Bacterial Strain Library"],"prefix":"10.1177","volume":"22","author":[{"given":"Si-Hyun","family":"Kim","sequence":"first","affiliation":[{"name":"The Catholic University of Korea"},{"name":"The Catholic University of Korea"},{"name":"The Catholic University of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chulmin","family":"Park","sequence":"additional","affiliation":[{"name":"The Catholic University of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hye-Sun","family":"Chun","sequence":"additional","affiliation":[{"name":"The Catholic University of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong-Gun","family":"Lee","sequence":"additional","affiliation":[{"name":"The Catholic University of Korea"},{"name":"The Catholic University of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae-Ki","family":"Choi","sequence":"additional","affiliation":[{"name":"The Catholic University of Korea"},{"name":"The Catholic University of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyo-Jin","family":"Lee","sequence":"additional","affiliation":[{"name":"The Catholic University of Korea"},{"name":"The Catholic University of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung-Yeon","family":"Cho","sequence":"additional","affiliation":[{"name":"The Catholic University of Korea"},{"name":"The Catholic University of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sun Hee","family":"Park","sequence":"additional","affiliation":[{"name":"The Catholic University of Korea"},{"name":"The Catholic University of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Su-Mi","family":"Choi","sequence":"additional","affiliation":[{"name":"The Catholic University of Korea"},{"name":"The Catholic University of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jung-Hyun","family":"Choi","sequence":"additional","affiliation":[{"name":"The Catholic University of Korea"},{"name":"The Catholic University of Korea"},{"name":"The Catholic University of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin-Hong","family":"Yoo","sequence":"additional","affiliation":[{"name":"The Catholic University of Korea"},{"name":"The Catholic University of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2016,3,14]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000153812.64956.EF"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0732-8893(00)00163-2"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.2.428-432.2000"},{"key":"e_1_3_3_5_2","unstructured":"Clinical and Laboratory Standards Institute. 2010. Performance Standards for Antimicrobial Susceptibility Testing: sixteenth informational supplement M100-S16. CLSI Wayne PA."},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.073262-0"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.1.24-27.1995"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01106-10"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2011.08.003"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-73.4.739"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01728-06"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01365-09"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00736-08"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkr563"},{"key":"e_1_3_3_15_2","unstructured":"Moody J.A. 2007. Synergism Testing: Broth Microdilution Checkerboard and Broth Macrodilution Methods. ASM Press Washington DC pp. 5.12.1\u20135.12.23."},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.7.2155-2161.2002"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00627-10"},{"key":"e_1_3_3_18_2","unstructured":"Pillai S.K. Eliopoulos G.M. 2005. Antimicrobial Combinations. Antibiotics in Laboratory Medicine. Fifth ed. Lippincott Williams & Wilkins Baltimore."},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/s15010-007-6316-4"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2012.10.028"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2006.01.004"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2012.12.006"}],"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/mdr.2015.0251","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2015.0251","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2015.0251","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T13:33:22Z","timestamp":1779197602000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/mdr.2015.0251"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,3,14]]},"references-count":21,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2016,7,1]]}},"alternative-id":["10.1089\/mdr.2015.0251"],"URL":"https:\/\/doi.org\/10.1089\/mdr.2015.0251","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,3,14]]}}}