{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T13:14:49Z","timestamp":1780406089296,"version":"3.54.1"},"reference-count":32,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2017,4,1]],"date-time":"2017-04-01T00:00:00Z","timestamp":1491004800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2017,4]]},"abstract":"\n The emergence and dissemination of NDM-1 (New Delhi metallo-\u03b2-lactamase-1)-producing\n Enterobacteriaceae<\/jats:italic>\n have resulted in a worldwide public health risk. This study described a high incidence and endemic spread of NDM-1-producing extensively drug-resistant\n Escherichia coli<\/jats:italic>\n in a teaching hospital in Zhejiang province, China. We recovered six nonduplicated NDM-1-producing\n E. coli<\/jats:italic>\n isolates from May 2014 to August 2014 with positive modified Hodge test and EDTA synergistic test. These isolates were highly resistant to \u03b2-lactam antimicrobials, aminoglycosides, and quinolones. PCR and DNA sequences analysis showed that all isolates carried the\n bla<\/jats:italic>\n NDM-1<\/jats:sub>\n ,\n bla<\/jats:italic>\n SHV-11<\/jats:sub>\n ,\n aac(6\u2032)-ib-cr<\/jats:italic>\n , and\n qnrB<\/jats:italic>\n . Several isolates also harbored\n bla<\/jats:italic>\n CTX-M-1<\/jats:sub>\n ,\n bla<\/jats:italic>\n CTX-M-9<\/jats:sub>\n ,\n rmtB<\/jats:italic>\n , and\n qnrA<\/jats:italic>\n . Southern blot confirmed that\n bla<\/jats:italic>\n NDM-1<\/jats:sub>\n was located on the same \u223c55\u2009kb plasmid and conjugation experiments further proved the contransferable characteristic of\n bla<\/jats:italic>\n NDM-1<\/jats:sub>\n . The\n ompC<\/jats:italic>\n sequences showed various mutations, which was related to multidrug resistance in\n E. coli<\/jats:italic>\n . Pulsed-field gel electrophoresis identified four of six isolates that belonged to the same genotype. Multilocus sequence typing assigned them to ST2, except one strain that belonged to ST594. Our study demonstrated that the resistance-associated genes and the loss of the outer membrane proteins could account for high resistance of NDM-1-producing\n E. coli<\/jats:italic>\n to multiple antimicrobial drugs. Both horizontal transfer of IncN and transmission of ST2 were responsible for the spread of drug resistance. These findings highlighted an urgent need to limit the further dissemination of NDM-1-producing\n E. coli<\/jats:italic>\n in this region.\n <\/jats:p>","DOI":"10.1089\/mdr.2015.0294","type":"journal-article","created":{"date-parts":[[2016,7,6]],"date-time":"2016-07-06T17:31:10Z","timestamp":1467826270000},"page":"272-279","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":7,"title":["Molecular Characterization and Epidemiologic Study of NDM-1-Producing Extensively Drug-Resistant\n Escherichia coli<\/i>"],"prefix":"10.1177","volume":"23","author":[{"given":"Jia","family":"Du","sequence":"first","affiliation":[{"name":"The First Affiliated Hospital of Wenzhou Medical University","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Li","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Wenzhou Medical University","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianming","family":"Cao","sequence":"additional","affiliation":[{"name":"Wenzhou Medical University","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Wu","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Wenzhou Medical University","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huale","family":"Chen","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Wenzhou Medical University","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanbo","family":"Hou","sequence":"additional","affiliation":[{"name":"Wenzhou Medical University","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"En","family":"Zhang","sequence":"additional","affiliation":[{"name":"Ningbo Medical Treatment Center Lihuili Hospital","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tieli","family":"Zhou","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Wenzhou Medical University","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2017,4]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"crossref","first-page":"422","DOI":"10.3390\/pathogens4030422","article-title":"Molecular characterization of the multidrug resistant Escherichia coli ST131 Clone","volume":"4","author":"Schembri M.A.","year":"2015","unstructured":"Schembri M.A., , Zakour N.L., , Phan M.D., , Forde B.M., , Stanton-Cook M, , and Beatson S.A. 2015. Molecular characterization of the multidrug resistant Escherichia coli ST131 Clone. Pathogens., 4:422\u2013430.","journal-title":"Pathogens."},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(11)70059-7"},{"key":"e_1_3_3_4_2","doi-asserted-by":"crossref","first-page":"1020","DOI":"10.1093\/jac\/dkn049","article-title":"Characterization of carbapenem-non-susceptible Escherichia coli isolates from a university hospital in Taiwan","volume":"61","author":"Liu Y.F.","year":"2008","unstructured":"Liu Y.F., , Yan J.J., , Ko W.C., , Tsai S.H., , and Wu J.J. 2008. Characterization of carbapenem-non-susceptible Escherichia coli isolates from a university hospital in Taiwan. J. Antimicrob. Chemother., 61:1020\u20131023.","journal-title":"J. Antimicrob. 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PLoS One., 6:e17989.","journal-title":"PLoS One."},{"key":"e_1_3_3_7_2","first-page":"e39","article-title":"Identification and characterization of a novel incompatibility group X3 plasmid carrying bla NDM-1 in Enterobacteriaceae isolates with epidemiological links to multiple geographical areas in China","volume":"1","author":"Ho P.L.","year":"2012","unstructured":"Ho P.L., , Li Z., , Lo W.U., , Cheung Y.Y., , Lin C.H., , Sham P.C., , Cheng V.C., , Ng T.K., , Que T.L., , and Chow K.H. 2012. Identification and characterization of a novel incompatibility group X3 plasmid carrying bla NDM-1 in Enterobacteriaceae isolates with epidemiological links to multiple geographical areas in China. Emerg. Microbes Infect., 1:e39.","journal-title":"Emerg. Microbes Infect."},{"key":"e_1_3_3_8_2","doi-asserted-by":"crossref","first-page":"4275","DOI":"10.1128\/AAC.02813-13","article-title":"High incidence and endemic spread of NDM-1-positive Enterobacteriaceae in Henan Province, China","volume":"58","author":"Qin S.","year":"2014","unstructured":"Qin S., , Fu Y., , Zhang Q., , Qi H., , Wen J.G., , Xu H., , Xu L., , Zeng L., , Tian H., , Rong L., , Li Y., , Shan L., , Yu Y., , Feng X., , and Liu H.M. 2014. High incidence and endemic spread of NDM-1-positive Enterobacteriaceae in Henan Province, China. Antimicrob. Agents Chemother., 58:4275\u20134282.","journal-title":"Antimicrob. 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