{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:26:43Z","timestamp":1777922803981,"version":"3.51.4"},"reference-count":17,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2017,3,1]],"date-time":"2017-03-01T00:00:00Z","timestamp":1488326400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:p>\n                    The expression of extended-spectrum beta-lactamases directly interferes with the treatment options in a clinical setting. It is not clearly defined why bacteria acquire multiple beta-lactamases and how they are being expressed in antibiotic stress. With this key question, the study was designed to understand the transcriptional response in\n                    <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                    harboring multiple\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>ESBLs<\/jats:sub>\n                    against different oxyimino-cephalosporin stress. A total of 169 consecutive, nonduplicate oxyimino-cephalosporin-resistant isolates of\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    were screened and were ESBL positive. Among them six isolates were found to harbor multiple beta-lactamase genes and we, as per our objective, selected them for this study. Molecular characterization was done by multiplex polymerase chain reaction (PCR) assay. Minimum inhibitory concentration, transcriptional expression, transferability, and plasmid incompatibility typing of multiple\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>ESBLs<\/jats:sub>\n                    were carried out. Plasmid stability and antibiotic susceptibility of donor and transconjugants were performed. A total of six isolates were found to be harboring multiple ESBL genes and MIC above the breakpoint level against all the tested antibiotics. Quantitative real-time PCR showed that in basal level without antibiotic stress, SHV-148 expressed more, but with ceftriaxone stressed, expression of CTX-M-15 and SHV-148 was high. In case of PER-1, expression was high with ceftazidime stress.\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>ESBLs<\/jats:sub>\n                    were horizontally transferable and originated through multiple inc types. Plasmids were stable till 115 serial passages. Pulsed-field gel electrophoresis results showed that multiple ESBL genes were spread through six pulsotypes. Our study concludes that acquisition of multiple ESBL genes in\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    was a specific adaptation for survival against multiple oxyimino-cephalosporin stress in this clinical setting.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2015.0340","type":"journal-article","created":{"date-parts":[[2016,5,23]],"date-time":"2016-05-23T16:00:38Z","timestamp":1464019238000},"page":"133-138","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":3,"title":["Transcriptional Response of Multiple ESBL Genes Within\n                    <i>Escherichia coli<\/i>\n                    Under Oxyimino-Cephalosporin Stress"],"prefix":"10.1177","volume":"23","author":[{"given":"Anand Prakash","family":"Maurya","sequence":"first","affiliation":[{"name":"Assam University","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Debadatta Dhar","family":"Chanda","sequence":"additional","affiliation":[{"name":"Silchar Medical College and Hospital","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Debajyoti","family":"Bora","sequence":"additional","affiliation":[{"name":"Chandra Kamal Bezbaruah Commerce College","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anupam","family":"Das Talukdar","sequence":"additional","affiliation":[{"name":"Assam University","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atanu","family":"Chakravarty","sequence":"additional","affiliation":[{"name":"Chandra Kamal Bezbaruah Commerce College","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amitabha","family":"Bhattacharjee","sequence":"additional","affiliation":[{"name":"Assam University","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2017,3]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"crossref","first-page":"4296","DOI":"10.1038\/ncomms5296","article-title":"Opposing effects of target over expression reveal drug mechanisms","volume":"5","author":"Palmer A.C.","year":"2014","unstructured":"Palmer A.C., , and Kishony R. 2014. Opposing effects of target over expression reveal drug mechanisms. Nat. Commun., 5:4296.","journal-title":"Nat. Commun."},{"key":"e_1_3_3_3_2","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1128\/AAC.39.1.185","article-title":"PCR mapping of integrons reveals several novel combinations of resistance genes","volume":"39","author":"Vesque C.","year":"1995","unstructured":"Vesque C., , Piche L., , Larose C., , and Roy P.H. 1995. PCR mapping of integrons reveals several novel combinations of resistance genes. Antimicrob. Agents Chemother., 39:185\u2013191.","journal-title":"Antimicrob. Agents Chemother."},{"key":"e_1_3_3_4_2","doi-asserted-by":"crossref","first-page":"4272","DOI":"10.1128\/JB.05413-11","article-title":"Genome of multidrug-resistant uropathogenic Escherichia coli strain NA114 from India","volume":"193","author":"Avasthi T.S.","year":"2011","unstructured":"Avasthi T.S., , Kumar N., , Baddam R., , Hussain A., , Nandanwar N., , Jadhav S., , and Ahmed N. 2011. Genome of multidrug-resistant uropathogenic Escherichia coli strain NA114 from India. J. Bacteriol., 193:4272\u20134273.","journal-title":"J. Bacteriol."},{"key":"e_1_3_3_5_2","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1128\/CMR.00125-13","article-title":"Escherichia coli ST131, an intriguing clonal group","volume":"27","author":"Chanoine N.M.H.","year":"2014","unstructured":"Chanoine N.M.H., , Bertrand X., , and Madec J.Y. 2014. Escherichia coli ST131, an intriguing clonal group. Clin. Microbiol. Rev., 27:543\u2013574.","journal-title":"Clin. Microbiol. Rev."},{"key":"e_1_3_3_6_2","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1093\/jac\/dkq463","article-title":"Molecular epidemiology of fluoroquinolone-resistant ST131 Escherichia coli producing CTX-M extended-spectrum beta-lactamases in nursing homes in Belfast, UK","volume":"66","author":"Dhanji H.","year":"2011","unstructured":"Dhanji H., , Doumith M., , Rooney P.J., , O'Leary M.C., , Loughrey A.C., , Hope R., , Woodford N., , and Livermore D.M. 2011. Molecular epidemiology of fluoroquinolone-resistant ST131 Escherichia coli producing CTX-M extended-spectrum beta-lactamases in nursing homes in Belfast, UK. J. Antimicrob. Chemother., 66:297\u2013303.","journal-title":"J. Antimicrob. Chemother."},{"key":"e_1_3_3_7_2","unstructured":"Clinical and Laboratory Standards Institute. 2014. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fourth Informational Supplement. CLSI Wayne PA."},{"key":"e_1_3_3_8_2","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1093\/jac\/dkf069","article-title":"Identification of PSE and OXA \u03b2-lactamase genes in Pseudomonas aeruginosa using PCR-restriction fragment length polymorphism","volume":"50","author":"Bert F.","year":"2002","unstructured":"Bert F., , Branger C., , and Zechovsky NL. 2002. Identification of PSE and OXA \u03b2-lactamase genes in Pseudomonas aeruginosa using PCR-restriction fragment length polymorphism. J. Antimicrob. Chemother., 50:11\u201318.","journal-title":"J. Antimicrob. Chemother."},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1097(03)00306-9"},{"key":"e_1_3_3_10_2","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1093\/jac\/dki160","article-title":"Prevalence of ambler class A and D \u03b2- lactamases among clinical isolates of Pseudomonas aeruginosa in Korea","volume":"56","author":"Lee S.","year":"2005","unstructured":"Lee S., , Park Y.J., , Kim M., , Lee H K., , Han K., , and Kang C.S. 2005. Prevalence of ambler class A and D \u03b2- lactamases among clinical isolates of Pseudomonas aeruginosa in Korea. J. Antimicrob. Chemother., 56:122\u2013127.","journal-title":"J. Antimicrob. Chemother."},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2005.03.018"},{"key":"e_1_3_3_12_2","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1128\/AAC.05420-11","article-title":"Genetic environment and stability of cfr in methicillin-resistant Staphylococcus aureus CM05","volume":"56","author":"Locke J.B.","year":"2012","unstructured":"Locke J.B., , Rahawi S., , LaMarre J., , Mankin L.S., , and Shawa K.J. 2012. Genetic environment and stability of cfr in methicillin-resistant Staphylococcus aureus CM05. Antimicrob. Agents Chemother., 56; 332\u2013340.","journal-title":"Antimicrob. Agents Chemother."},{"key":"e_1_3_3_13_2","doi-asserted-by":"crossref","first-page":"6823","DOI":"10.1093\/nar\/19.24.6823","article-title":"Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes","volume":"19","author":"Versalovic J.","year":"1991","unstructured":"Versalovic J., , Koeuth T., , and Lupski J.R. 1991. Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acid Res., 19:6823\u2013683.","journal-title":"Nucleic Acid Res."},{"key":"e_1_3_3_14_2","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1128\/CMR.14.4.933-951.2001","article-title":"Extended spectrum \u03b2-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat","volume":"14","author":"Bradford P.A.","year":"2001","unstructured":"Bradford P.A. 2001. Extended spectrum \u03b2-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat. Clin. Microbiol. Rev., 14:933\u2013951.","journal-title":"Clin. Microbiol. Rev."},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2013.02.008"},{"key":"e_1_3_3_16_2","doi-asserted-by":"crossref","first-page":"2249","DOI":"10.1093\/jac\/dkt208","article-title":"Extended-spectrum cephalosporin-resistant Escherichia coli in community, specialized outpatient clinic and hospital settings in Switzerland","volume":"88","author":"Seiffert S.N.","year":"2013","unstructured":"Seiffert S.N., , Hilty M., , Kronenberg A., , Droz S., , Perreten V., , and Endimiani A. 2013. Extended-spectrum cephalosporin-resistant Escherichia coli in community, specialized outpatient clinic and hospital settings in Switzerland. J. Antimicrob. Chemother., 88:2249\u20132254.","journal-title":"J. Antimicrob. Chemother."},{"key":"e_1_3_3_17_2","doi-asserted-by":"crossref","first-page":"5933","DOI":"10.1128\/AAC.00639-11","article-title":"Complete sequence of pJIE 143, a pir type plasmid carrying ISEcp1-bla\n                  CTX-M-15 from an Escherichia coli ST131 isolate","volume":"55","author":"Partridge S.R.","year":"2011","unstructured":"Partridge S.R., , Ellem J.A., , Tetu S.G., , Zong Z., , Paulsen I.T., , and Iredell Z.R. 2011. Complete sequence of pJIE 143, a pir type plasmid carrying ISEcp1-blaCTX-M-15 from an Escherichia coli ST131 isolate. Antimicrob. Agents Chemother., 55:5933\u20135935.","journal-title":"Antimicrob. Agents Chemother."},{"key":"e_1_3_3_18_2","doi-asserted-by":"crossref","first-page":"195","DOI":"10.3201\/eid1402.070350","article-title":"Dissemination of clonally related Escherichia coli strains expressing extended spectrum \u03b2-lactamase CTX-M-15","volume":"14","author":"Coque T.M.","year":"2008","unstructured":"Coque T.M., , Novais A., , Carattoli A., , Poirel L., , Pitout J., , Peixe L., , Baquero F., , R .Canton, and Nordmann P. 2008. Dissemination of clonally related Escherichia coli strains expressing extended spectrum \u03b2-lactamase CTX-M-15. Emerg. Infect. Dis., 14:195\u2013200.","journal-title":"Emerg. Infect. Dis."}],"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/mdr.2015.0340","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2015.0340","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2015.0340","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:30:14Z","timestamp":1777667414000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/mdr.2015.0340"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,3]]},"references-count":17,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2017,3]]}},"alternative-id":["10.1089\/mdr.2015.0340"],"URL":"https:\/\/doi.org\/10.1089\/mdr.2015.0340","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,3]]}}}