{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T18:04:45Z","timestamp":1792087485382,"version":"4.4.0"},"reference-count":50,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2016,12,1]],"date-time":"2016-12-01T00:00:00Z","timestamp":1480550400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:p>\n                    Plasmids play a key role in the genetic plasticity and survival of\n                    <jats:italic toggle=\"yes\">Staphylococcus aureus<\/jats:italic>\n                    in challenging environments. Although many\n                    <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                    plasmids have been described, still few studies portray the plasmid content of a given\n                    <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                    population. The aim of this work was to characterize the plasmids carried by a collection of 53\n                    <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                    isolates collected in a large hospital in Lisbon, Portugal, and investigate their role in conferring resistance to several antimicrobial agents. Plasmids were present in 44 out of the 53 isolates and were grouped into eleven\n                    <jats:italic toggle=\"yes\">Acc<\/jats:italic>\n                    I restriction profiles. Plasmid curing of representative strains and comparison of antimicrobial susceptibility profiles between pairs of isogenic strains proved to be a valuable guidance tool in the identification of plasmid-located resistance genes. The plasmids harbored several resistance genes, namely\n                    <jats:italic toggle=\"yes\">blaZ<\/jats:italic>\n                    (resistance to \u03b2-lactams),\n                    <jats:italic toggle=\"yes\">erm<\/jats:italic>\n                    (C) (resistance to macrolides, lincosamides, and streptogramin B),\n                    <jats:italic toggle=\"yes\">cadA<\/jats:italic>\n                    (resistance to cadmium and zinc),\n                    <jats:italic toggle=\"yes\">cadD<\/jats:italic>\n                    (resistance to cadmium), and\n                    <jats:italic toggle=\"yes\">qacA<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">smr<\/jats:italic>\n                    (resistance to biocides and dyes). This study demonstrates the impact of plasmids on the resistance properties of\n                    <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                    , highlighting their role in the dissemination of antibiotic, heavy metal, and biocide resistance genes, and survival of this major pathogen in the hospital environment.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2015.0352","type":"journal-article","created":{"date-parts":[[2016,6,16]],"date-time":"2016-06-16T20:26:50Z","timestamp":1466108810000},"page":"617-626","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":18,"title":["Plasmid-Borne Antimicrobial Resistance of\n                    <i>Staphylococcus aureus<\/i>\n                    Isolated in a Hospital in Lisbon, Portugal"],"prefix":"10.1177","volume":"22","author":[{"given":"Sofia Santos","family":"Costa","sequence":"first","affiliation":[{"name":"Universidade Nova de Lisboa","place":["Portugal"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cl\u00e1udia","family":"Palma","sequence":"additional","affiliation":[{"name":"Universidade Nova de Lisboa","place":["Portugal"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristina","family":"Kadlec","sequence":"additional","affiliation":[{"name":"Institute of Farm Animal Genetics","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea T.","family":"Fessler","sequence":"additional","affiliation":[{"name":"Institute of Farm Animal Genetics","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel","family":"Viveiros","sequence":"additional","affiliation":[{"name":"Universidade Nova de Lisboa","place":["Portugal"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"Melo-Cristino","sequence":"additional","affiliation":[{"name":"Centro Hospitalar Lisboa Norte E.P.E., Instituto de Microbiologia, Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisbon, Portugal."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Schwarz","sequence":"additional","affiliation":[{"name":"Institute of Farm Animal Genetics","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Couto","sequence":"additional","affiliation":[{"name":"Universidade Nova de Lisboa","place":["Portugal"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2016,12]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2200"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.09.30"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2013.02.006"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2014.10.002"},{"key":"e_1_3_3_6_2","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.ijmm.2013.11.010","article-title":"Staphylococcus aureus genomics and the impact of horizontal gene transfer","volume":"304","author":"Lindsay J.A.","year":"2014","unstructured":"Lindsay J.A. 2014. Staphylococcus aureus genomics and the impact of horizontal gene transfer. Int. J. Med. Microbiol., 304:103\u2013109.","journal-title":"Int. J. Med. Microbiol."},{"key":"e_1_3_3_7_2","first-page":"2","article-title":"Plasmid-mediated antimicrobial resistance in staphylococci and other Firmicutes","author":"Schwarz S.","year":"2014","unstructured":"Schwarz S., , Shen J., , Wendlandt S., , Fe\u03b2ler A.T., , Wang Y., , Kadlec K., , and Wu C.-M. 2014. Plasmid-mediated antimicrobial resistance in staphylococci and other Firmicutes. Microbiol. Spectr. 2.","journal-title":"Microbiol. Spectr."},{"key":"e_1_3_3_8_2","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1146\/annurev.mi.43.100189.002541","article-title":"Staphylococcal plasmids and their replication","volume":"43","author":"Novick R.P.","year":"1989","unstructured":"Novick R.P. 1989. Staphylococcal plasmids and their replication. Annu. Rev. Microbiol., 43:537\u2013565.","journal-title":"Annu. Rev. 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