{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:26:50Z","timestamp":1777922810466,"version":"3.51.4"},"reference-count":19,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2017,4,1]],"date-time":"2017-04-01T00:00:00Z","timestamp":1491004800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:p>\n                    A novel assay for rapid determination of resistance to antibiotic inhibitors of protein synthesis was developed for the gram-positive pathogens,\n                    <jats:italic toggle=\"yes\">Enterococcus faecalis<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">Streptococcus pneumoniae.<\/jats:italic>\n                    To this purpose, a lytic response was obtained by a brief incubation with lysozyme or a mixture of lysozyme, Triton X-100, and EDTA for\n                    <jats:italic toggle=\"yes\">E. faecalis<\/jats:italic>\n                    (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    \u2009=\u200982) and\n                    <jats:italic toggle=\"yes\">S. pneumoniae<\/jats:italic>\n                    (\n                    <jats:italic toggle=\"yes\">n<\/jats:italic>\n                    \u2009=\u200951), respectively. Lysis was quantified by visualizing the released nucleoids. Antibiotic-susceptible bacteria treated with Clinical and Laboratory Standards Institute (CLSI) breakpoint doses of erythromycin, azithromycin, or doxycycline that inhibited protein synthesis demonstrated a large reduction of lysed cells with respect to the control, that is, without antibiotics. However, cell lysis prevention was much lower in nonsusceptible strains, with unsuccessful inhibition of protein synthesis. ROC analysis showed that a reduction value of \u226535.6% and \u226540.4% discriminates susceptible and nonsusceptible strains for erythromycin and for doxycycline, respectively, in\n                    <jats:italic toggle=\"yes\">E. faecalis<\/jats:italic>\n                    , whereas \u226520.0% is adequate for both macrolides and doxycycline in\n                    <jats:italic toggle=\"yes\">S. pneumoniae<\/jats:italic>\n                    . Resistant stains were identified in 90\u2013120\u2009min with sensitivity and specificity between 91.7% and 100%. This is a proof of concept that evaluation of the lytic response may be a rapid and efficient test for determination of resistance to antibiotic inhibitors of protein synthesis.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2016.0091","type":"journal-article","created":{"date-parts":[[2016,7,6]],"date-time":"2016-07-06T17:32:02Z","timestamp":1467826322000},"page":"267-271","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["Rapid Assessment of Resistance to Antibiotic Inhibitors of Protein Synthesis in the Gram-Positive Pathogens,\n                    <i>Enterococcus faecalis<\/i>\n                    and\n                    <i>Streptococcus pneumoniae<\/i>\n                    , Based on Evaluation of the Lytic Response"],"prefix":"10.1177","volume":"23","author":[{"given":"F\u00e1tima","family":"Otero","sequence":"first","affiliation":[{"name":"Complexo Hospitalario Universitario de A Coru\u00f1a (CHUAC)","place":["Spain"]},{"name":"Centro Oncol\u00f3gico de Galicia","place":["Spain"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda","family":"Tamayo","sequence":"additional","affiliation":[{"name":"Complexo Hospitalario Universitario de A Coru\u00f1a (CHUAC)","place":["Spain"]},{"name":"Centro Oncol\u00f3gico de Galicia","place":["Spain"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebeca","family":"Santiso","sequence":"additional","affiliation":[{"name":"Complexo Hospitalario Universitario de A Coru\u00f1a (CHUAC)","place":["Spain"]},{"name":"Centro Oncol\u00f3gico de Galicia","place":["Spain"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaime","family":"Gos\u00e1lvez","sequence":"additional","affiliation":[{"name":"Universidad Aut\u00f3noma de Madrid","place":["Spain"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Germ\u00e1n","family":"Bou","sequence":"additional","affiliation":[{"name":"Complexo Hospitalario Universitario de A Coru\u00f1a (CHUAC)","place":["Spain"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Luis","family":"Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Complexo Hospitalario Universitario de A Coru\u00f1a (CHUAC)","place":["Spain"]},{"name":"Centro Oncol\u00f3gico de Galicia","place":["Spain"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2017,4]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.3.1.46"},{"key":"e_1_3_3_3_2","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1086\/591861","article-title":"NHSH annual update: antimicrobial-resistant pathogens associated with healthcare-associated infections: annual summary of data reported to the national healthcare safety network at the centers for disease control and prevention, 2006\u20132007","volume":"29","author":"Hidron A.I.","year":"2008","unstructured":"Hidron A.I., , Edwards J.R., , Patel J., , Horan T.C., , Sievert D.M., , Pollock D.A., et al. 2008. NHSH annual update: antimicrobial-resistant pathogens associated with healthcare-associated infections: annual summary of data reported to the national healthcare safety network at the centers for disease control and prevention, 2006\u20132007. Infect. Control Hosp. Epidemiol., 29:996\u20131011.","journal-title":"Infect. Control Hosp. Epidemiol."},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.12703\/P6-82"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.4161\/viru.21282"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1086\/320181"},{"key":"e_1_3_3_7_2","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1056\/NEJMp1215093","article-title":"The future of antibiotics and resistance","volume":"368","author":"Spellber B.","year":"2013","unstructured":"Spellber B., , Barlett J.G., , and Gilbert D.N. 2013. The future of antibiotics and resistance. New Engl. J. Med., 368:299\u2013302.","journal-title":"New Engl. J. 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