{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T06:57:41Z","timestamp":1791615461621,"version":"4.3.4"},"reference-count":32,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2017,7,1]],"date-time":"2017-07-01T00:00:00Z","timestamp":1498867200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2017,7]]},"abstract":"\n Objective:<\/jats:title>\n \n We aimed to investigate whether mutations and\/or overexpressions of\n ERG4<\/jats:italic>\n and\n ERG11<\/jats:italic>\n genes were involved in drug resistance to azoles in\n Candida albicans<\/jats:italic>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Methods:<\/jats:title>\n \n Totally, 34 clinical isolates of\n C. albicans<\/jats:italic>\n were included in this study. Antimicrobial susceptibility tests, including 5-fluorocytosine (5-FC), amphotericin B (AMB), fluconazole (FCA), itraconazole (ITR), and voriconazole (VRC), were performed by broth microdilution method. Mutations in the\n ERG4<\/jats:italic>\n and\n ERG11<\/jats:italic>\n genes sequence were detected. The messenger RNA (mRNA) levels of\n ERG4<\/jats:italic>\n and\n ERG11<\/jats:italic>\n were measured by quantitative real-time polymerase chain reaction. The correlation of the expression levels of\n ERG4<\/jats:italic>\n with\n ERG11<\/jats:italic>\n genes in susceptible isolates and resistant isolates was analyzed by Pearson's correlation analysis.\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n Among 34\n C. albicans<\/jats:italic>\n isolates, 52.94%, 70.59%, and 50.00% isolates were resistant to FCA, ITR, and VRC, respectively. Sequencing results revealed that only 2 silent mutations were found in\n ERG4<\/jats:italic>\n gene, while 10 amino acid substitutions, including 6 reported previously and 4 new identified, were frequently found in\n ERG11<\/jats:italic>\n gene. The mRNA levels of\n ERG4<\/jats:italic>\n and\n ERG11<\/jats:italic>\n genes were significantly elevated in resistant compared with susceptible\n C. albicans<\/jats:italic>\n isolates. Furthermore, the mRNA level of\n ERG4<\/jats:italic>\n was positively correlated with\n ERG11<\/jats:italic>\n in susceptible but not resistant\n C. albicans<\/jats:italic>\n isolates.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions:<\/jats:title>\n \n The resistance to azoles may be associated with the mutations in\n ERG11<\/jats:italic>\n but not\n ERG4<\/jats:italic>\n gene in\n C. albicans<\/jats:italic>\n isolates. In addition, overexpressed\n ERG4<\/jats:italic>\n and\n ERG11<\/jats:italic>\n genes are found in resistant\n C. albicans<\/jats:italic>\n isolates, and the mRNA levels of\n ERG4<\/jats:italic>\n may be irrelevant to\n ERG11<\/jats:italic>\n in resistant\n C. albicans<\/jats:italic>\n isolates.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1089\/mdr.2016.0095","type":"journal-article","created":{"date-parts":[[2016,12,15]],"date-time":"2016-12-15T11:51:00Z","timestamp":1481802660000},"page":"563-570","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":42,"title":["Mutations and\/or Overexpressions of\n ERG4<\/i>\n and\n ERG11<\/i>\n Genes in Clinical Azoles-Resistant Isolates of\n Candida albicans<\/i>"],"prefix":"10.1177","volume":"23","author":[{"given":"Wenli","family":"Feng","sequence":"first","affiliation":[{"name":"The Second Hospital of Shanxi Medical University","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Yang","sequence":"additional","affiliation":[{"name":"The Second Hospital of Shanxi Medical University","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiqin","family":"Xi","sequence":"additional","affiliation":[{"name":"The Second Hospital of Shanxi Medical University","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zusha","family":"Qiao","sequence":"additional","affiliation":[{"name":"The Second Hospital of Shanxi Medical University","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaping","family":"Lv","sequence":"additional","affiliation":[{"name":"The Second Hospital of Shanxi Medical University","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiru","family":"Wang","sequence":"additional","affiliation":[{"name":"The Second Hospital of Shanxi Medical University","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Ma","sequence":"additional","affiliation":[{"name":"The Second Hospital of Shanxi Medical University","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanqing","family":"Wang","sequence":"additional","affiliation":[{"name":"The Second Hospital of Shanxi Medical University","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Cen","sequence":"additional","affiliation":[{"name":"The Second Hospital of Shanxi Medical University","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2017,7]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.2147\/TCRM.S40160"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00029-06"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00041-08"},{"key":"e_1_3_3_5_2","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1080\/02681219480000821","article-title":"Mechanisms and clinical impact of antifungal drug resistance","volume":"32","author":"Vanden Bossche H.","year":"1994","unstructured":"Vanden Bossche H., , Warnock D., , Dupont B., , Kerridge D., , Gupta S.S., , Improvisi L., , Marichal P., , Odds F., , Provost F., , and Ronin O.. 1994. 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