{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:26:46Z","timestamp":1777922806990,"version":"3.51.4"},"reference-count":19,"publisher":"SAGE Publications","issue":"7","license":[{"start":{"date-parts":[[2017,2,2]],"date-time":"2017-02-02T00:00:00Z","timestamp":1485993600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2017,10,1]]},"abstract":"\n Antimicrobial resistance poses a great challenge to clinicians leaving very limited treatment options available. A panel of carbapenem-resistant bacterial isolates was selected based on Rapidec Carba NP test from a total of 900 samples, which were collected from different specialities hospitals of Kanpur, India. Carba NP-positive isolates were screened for carbapenemases, extended-spectrum beta-lactamases (ESBLs), quinolone resistance, and 16s methyltransferase genes. Presence of diverse\n bla<\/jats:italic>\n NDM<\/jats:sub>\n (\n bla<\/jats:italic>\n NDM-1<\/jats:sub>\n and\n bla<\/jats:italic>\n NDM-5<\/jats:sub>\n ) were detected and horizontal transferability was determined by transformation and conjugation assay. Elimination of\n bla<\/jats:italic>\n NDM-1<\/jats:sub>\n and\n bla<\/jats:italic>\n NDM-5<\/jats:sub>\n harboring plasmid was done by treating the isolates with sodium dodecyl sulfate. The transcriptional response of\n bla<\/jats:italic>\n NDM-1<\/jats:sub>\n and\n bla<\/jats:italic>\n NDM-5<\/jats:sub>\n under the exposure of imipenem, meropenem, and ertapenem stress was determined by quantitative real-time polymerase chain reaction.\n bla<\/jats:italic>\n NDM<\/jats:sub>\n harboring isolates were found to be horizontally transferable through IncF\n rep<\/jats:sub>\n B and K type plasmid and could be successfully eliminated after the single treatment with sodium dodecyl sulfate. A distinct pattern of transcriptional response was observed for\n bla<\/jats:italic>\n NDM-1<\/jats:sub>\n and\n bla<\/jats:italic>\n NDM-5<\/jats:sub>\n under the pressure of carbapenem antibiotics where an upregulated expression of both\n bla<\/jats:italic>\n NDM-1<\/jats:sub>\n and\n bla<\/jats:italic>\n NDM-5<\/jats:sub>\n was observed. Minimum inhibitory concentration (MIC) results revealed that\n bla<\/jats:italic>\n NDM<\/jats:sub>\n harboring strains showed a high MIC range against imipenem, meropenem, ertapenem, cefepime, and aztreonam. Thus, prudent action should be taken to control the spread of these multidrug-resistant determinants or at least slowing down their lateral transfer in the hospital settings.\n <\/jats:p>","DOI":"10.1089\/mdr.2016.0124","type":"journal-article","created":{"date-parts":[[2017,2,2]],"date-time":"2017-02-02T11:30:35Z","timestamp":1486035035000},"page":"815-821","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":13,"title":["Occurrence of\n bla<\/i>\n NDM-1<\/sub>\n and\n bla<\/i>\n NDM-5<\/sub>\n in a Tertiary Referral Hospital of North India"],"prefix":"10.1177","volume":"23","author":[{"given":"Deepjyoti","family":"Paul","sequence":"first","affiliation":[{"name":"Assam University","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atul","family":"Garg","sequence":"additional","affiliation":[{"name":"GSVM Medical College","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amitabha","family":"Bhattacharjee","sequence":"additional","affiliation":[{"name":"Assam University","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2017,2,2]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2012.02.017"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03363-13"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkq273"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01783-15"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12941-016-0146-0"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.05045-11"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.4.1406-1409.2004"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0145823"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00117-13"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00748-08"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00016-09"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.plasmid.2012.03.001"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-2508(00)00140-6"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00996-10"},{"key":"e_1_3_4_16_2","unstructured":"Clinical Laboratory Standard Institute. 2011. 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