{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:26:56Z","timestamp":1777922816867,"version":"3.51.4"},"reference-count":81,"publisher":"SAGE Publications","issue":"7","license":[{"start":{"date-parts":[[2017,2,24]],"date-time":"2017-02-24T00:00:00Z","timestamp":1487894400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2017,10,1]]},"abstract":"<jats:p>\n                    Nonfermenting Gram-negative bacteria such as\n                    <jats:italic toggle=\"yes\">Pseudomonas aeruginosa<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">Acinetobacter baumannii<\/jats:italic>\n                    are widespread in the environment and are increasingly associated with nosocomial infections, often associated with multidrug-resistance phenotypes. This study aimed to evaluate epidemiological, physiological, and molecular characteristics of carbapenem resistance in\n                    <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">A. baumannii.<\/jats:italic>\n                    In total, 63 nonreplicated strains (44\n                    <jats:italic toggle=\"yes\">A. baumannii<\/jats:italic>\n                    and 19\n                    <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                    ) were isolated from hospitalized patients. Antimicrobial resistance patterns, biocide tolerance, oxidative stress, hemolytic activity, and biofilm formation were assessed. Genetic markers related to \u03b2-lactamase synthesis, efflux systems, and porin loss were screened by PCR. Epidemiological data of patients were analyzed. Advanced age, intensive care unit admission, invasive medical devices, treatment with fluoroquinolones or \u03b2-lactams\/\u03b2-lactamase inhibitor combinations, and prolonged hospital stay were predisposing factors for infection. Colistin showed to be active\n                    <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                    against these bacteria. Carbapenem-resistant\n                    <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                    strains did not show hemolytic activity and were less tolerant to oxidative stress and biocides. However, increased ability of biofilm formation was observed, comparing to the carbapenem-susceptible isolates. Genetic markers related to oxacillinases synthesis (OXA-23 and OXA-143),\n                    <jats:italic toggle=\"yes\">oprD<\/jats:italic>\n                    absence, and efflux pump (\n                    <jats:italic toggle=\"yes\">ade<\/jats:italic>\n                    B) were detected in carbapenem-resistant\n                    <jats:italic toggle=\"yes\">A. baumannii.<\/jats:italic>\n                    Screening for OXA-51-like gene was performed as confirmatory test for\n                    <jats:italic toggle=\"yes\">A. baumannii<\/jats:italic>\n                    identification. In\n                    <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                    genes encoding efflux pumps (MexAB-OprM, MexCD-OprJ, MexEF-OprN, and MexXY-OprM) and SPM-1 were found; besides,\n                    <jats:italic toggle=\"yes\">oprD<\/jats:italic>\n                    absence was also observed. Our results suggest that these organisms are well adapted to different environments and confirm the difficulty of therapeutic management of patients with infections associated with multidrug-resistant microorganisms, with direct impact on mortality and epidemiological control of these strains in health centers.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2016.0219","type":"journal-article","created":{"date-parts":[[2017,2,24]],"date-time":"2017-02-24T11:17:00Z","timestamp":1487935020000},"page":"852-863","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":21,"title":["Epidemiological, Physiological, and Molecular Characteristics of a Brazilian Collection of Carbapenem-Resistant\n                    <i>Acinetobacter baumannii<\/i>\n                    and\n                    <i>Pseudomonas aeruginosa<\/i>"],"prefix":"10.1177","volume":"23","author":[{"given":"Vanessa Cordeiro","family":"Dias","sequence":"first","affiliation":[{"name":"Federal University of Juiz de Fora","place":["Brazil"]},{"name":"Cortes Villela Clinical Laboratory","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juliana Alves","family":"Resende","sequence":"additional","affiliation":[{"name":"Federal University of Juiz de Fora","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9 Netto","family":"Bastos","sequence":"additional","affiliation":[{"name":"Cortes Villela Clinical Laboratory","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucas Quinet","family":"De Andrade Bastos","sequence":"additional","affiliation":[{"name":"Cortes Villela Clinical Laboratory","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victor Quinet","family":"De Andrade Bastos","sequence":"additional","affiliation":[{"name":"Cortes Villela Clinical Laboratory","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo Villela","family":"Bastos","sequence":"additional","affiliation":[{"name":"Cortes Villela Clinical Laboratory","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cl\u00e1udio Galuppo","family":"Diniz","sequence":"additional","affiliation":[{"name":"Federal University of Juiz de Fora","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V\u00e2nia L\u00facia","family":"Da Silva","sequence":"additional","affiliation":[{"name":"Federal University of Juiz de Fora","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2017,2,24]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1590\/S1413-86702010000500002"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/504477"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/504487"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0037-86822010000400006"},{"key":"e_1_3_3_6_2","first-page":"317","article-title":"Distribution of bla\n                  OXA genes among carbapenem resistant Acinetobacter baumannii nosocomial strains in Poland","volume":"35","author":"Nowak P.","year":"2012","unstructured":"Nowak P., , Paluchowska P., , and Budak A.. 2012. 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