{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T07:46:57Z","timestamp":1789544817077,"version":"build-2803163510"},"reference-count":48,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2018,4,1]],"date-time":"2018-04-01T00:00:00Z","timestamp":1522540800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:p>\n                    <jats:italic toggle=\"yes\">Campylobacter<\/jats:italic>\n                    spp. is a major cause of foodborne diseases in humans, particularly when transmitted by the handling or consumption of undercooked poultry meat. Most\n                    <jats:italic toggle=\"yes\">Campylobacter<\/jats:italic>\n                    infections are self-limiting, but antimicrobial treatment (\n                    <jats:italic toggle=\"yes\">e.g<\/jats:italic>\n                    ., fluoroquinolones and macrolides) is necessary in severe or prolonged cases. The indiscriminate use of these drugs, both in clinical medicine and animal production, has a major impact on public health. The aim of the present study was to identify\n                    <jats:italic toggle=\"yes\">Campylobacter<\/jats:italic>\n                    strains, isolated from turkey and broilers, using both PCR and the matrix-assisted laser desorption\u2013ionization time-of-flight (MALDI-TOF) methods to reveal the accuracy of identification, as well to evaluate the antimicrobial and genetic resistance of the investigated strains. MALDI-TOF and PCR methods were used to show differences, if any, in the specificity of that test. In this study, MALDI-TOF mass spectrometry gave the same results as multiplex PCR, in all cases. The highest rate of resistance (\n                    <jats:italic toggle=\"yes\">i.e<\/jats:italic>\n                    ., 100% of turkey and broiler strains) was detected against ciprofloxacin, whereas 58.1% of turkey and 78.6% of broiler strains were resistant to tetracycline. Multidrug-resistant isolates were not found in the study. All ciprofloxacin-resistant strains had a mutation in the\n                    <jats:italic toggle=\"yes\">gyrA<\/jats:italic>\n                    gene, at the Thr-86 position. The presence of the\n                    <jats:italic toggle=\"yes\">tetO<\/jats:italic>\n                    gene was found in 71% of turkey and in 100% of broiler strains. All resistant to tetracycline strains included\n                    <jats:italic toggle=\"yes\">tetO<\/jats:italic>\n                    gene. Additionally, in five turkey and three broiler strains, susceptible to tetracycline,\n                    <jats:italic toggle=\"yes\">tetO<\/jats:italic>\n                    gene was present. These results indicate the high prevalence of\n                    <jats:italic toggle=\"yes\">Campylobacter<\/jats:italic>\n                    strains, which are phenotypically and genetically resistant to fluoroquinolones and tetracycline.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2016.0249","type":"journal-article","created":{"date-parts":[[2017,6,19]],"date-time":"2017-06-19T17:06:08Z","timestamp":1497891968000},"page":"314-322","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":58,"title":["High Prevalence of Resistance to Fluoroquinolones and Tetracycline\n                    <i>Campylobacter<\/i>\n                    Spp. Isolated from Poultry in Poland"],"prefix":"10.1177","volume":"24","author":[{"given":"Anna","family":"Wo\u017aniak-Biel","sequence":"first","affiliation":[{"name":"Wroclaw University of Environmental and Life Sciences","place":["Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela","family":"Bugla-P\u0142osko\u0144ska","sequence":"additional","affiliation":[{"name":"University of Wroclaw","place":["Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alicja","family":"Kielsznia","sequence":"additional","affiliation":[{"name":"University of Wroclaw","place":["Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamila","family":"Korzekwa","sequence":"additional","affiliation":[{"name":"University of Wroclaw","place":["Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Tobiasz","sequence":"additional","affiliation":[{"name":"Institute of Immunology and Experimental Therapy","place":["Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnieszka","family":"Korzeniowska-Kowal","sequence":"additional","affiliation":[{"name":"Institute of Immunology and Experimental Therapy","place":["Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alina","family":"Wieliczko","sequence":"additional","affiliation":[{"name":"Wroclaw University of Environmental and Life Sciences","place":["Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2018,4]]},"reference":[{"key":"e_1_3_3_2_2","unstructured":"World Health Organization. 2012. 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