{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:27:18Z","timestamp":1777922838008,"version":"3.51.4"},"reference-count":36,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2018,4,1]],"date-time":"2018-04-01T00:00:00Z","timestamp":1522540800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:p>\n                    <jats:italic toggle=\"yes\">gyrA<\/jats:italic>\n                    is a clinically validated therapeutic drug target that catalyzes changes in DNA topology by introducing negative supercoils into DNA. The broad-spectrum quinolones are hitherto the most potent antibacterial agents targeted toward\n                    <jats:italic toggle=\"yes\">gyrA<\/jats:italic>\n                    . Increasing resistance to quinolones is accompanied by several mutations in the quinolone resistance-determining region of\n                    <jats:italic toggle=\"yes\">gyrA<\/jats:italic>\n                    . In the present study, we report a comprehensive picture of the dynamic behavior of wild-type, T86I, and T86I\/P104S mutants (MTs) of\n                    <jats:italic toggle=\"yes\">gyrA<\/jats:italic>\n                    from\n                    <jats:italic toggle=\"yes\">Campylobacter jejuni<\/jats:italic>\n                    in complex with ciprofloxacin to unravel the atomistic details of the mechanism underlying resistance to quinolones. Our simulation results reveal that no substantial conformational changes were observed. It was observed that these mutations disrupted residue interaction network landscape to a significant extent, which would affect ligand binding affinity. A distinctive pattern of dominant motions was clearly discernible in wild and MT\n                    <jats:italic toggle=\"yes\">gyrA<\/jats:italic>\n                    forms. The results reported in this study associate\n                    <jats:italic toggle=\"yes\">gyrA<\/jats:italic>\n                    mutations with quinolone resistance and would pave a way for facilitating wet laboratory researchers to develop\n                    <jats:italic toggle=\"yes\">gyrA<\/jats:italic>\n                    MT-based therapeutic strategies against resistant pathogens.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2017.0020","type":"journal-article","created":{"date-parts":[[2017,7,31]],"date-time":"2017-07-31T15:04:19Z","timestamp":1501513459000},"page":"232-243","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["Molecular Dynamics Simulations of Quinolone Resistance-Associated T86I and P104S Mutations in\n                    <i>Campylobacter jejuni<\/i>\n                    <i>gyrA<\/i>\n                    : Unraveling Structural Repercussions"],"prefix":"10.1177","volume":"24","author":[{"given":"Kusum","family":"Mehla","sequence":"first","affiliation":[{"name":"Jaypee University of Information Technology","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jayashree","family":"Ramana","sequence":"additional","affiliation":[{"name":"Jaypee University of Information Technology","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2018,4]]},"reference":[{"key":"e_1_3_2_2_2","unstructured":"Friedman C.R. 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