{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T06:27:10Z","timestamp":1782368830134,"version":"3.54.5"},"reference-count":31,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2017,5,3]],"date-time":"2017-05-03T00:00:00Z","timestamp":1493769600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2017,12,1]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives:<\/jats:title>\n                    <jats:p>\n                      We aim to investigate, as a first insight, the presence and rates of high-risk\n                      <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                      ST131 clone in Istanbul and evaluate antimicrobial resistance and CTX-M-15 production of ST131 and non-ST131 isolates. The use of MALDI-TOF MS (matrix-assisted laser desorption\/ionization time-of-flight mass-spectrometry) to detect\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      ST131 clone is also evaluated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>\n                      A total of 203 extended-spectrum beta-lactamase (ESBL)-producing urinary isolates from a training hospital in Istanbul were investigated. Detection of\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      ST131 was done by MALDI-TOF MS and real-time PCR melting curve analysis. The presence of CTX-M and CTX-M-15 beta-lactamases was investigated by PCR and sequence analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>Of the 203 isolates, 81 (39.9%) and 75 (36.9%) isolates were identified as ST131 clone by PCR and MALDI-TOF MS, respectively. Resistance to ciprofloxacin was significantly higher among ST131 isolates. A total of 169 (83.5%) isolates produced CTX-M beta-lactamase, of which 72 (43%) were CTX-M-15. The production of CTX-M and CTX-M-15 were significantly higher among ST131 isolates.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>\n                      We have demonstrated, for the first time, high rates of ST131 clone among ESBL-producing\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      isolates in Istanbul, a region with high rates of resistance to third-generation cephalosporins and fluoroquinolones. Further investigation of this high-risk clone and its contribution to high antimicrobial resistance in Turkey is essential. MALDI-TOF MS is a useful tool for detection of high-risk clones and associated resistance patterns, simultaneous to bacterial identification.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/mdr.2017.0021","type":"journal-article","created":{"date-parts":[[2017,5,3]],"date-time":"2017-05-03T11:11:07Z","timestamp":1493809867000},"page":"1032-1036","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":9,"title":["A First Insight into\n                    <i>Escherichia coli<\/i>\n                    ST131 High-Risk Clone Among Extended-Spectrum Beta-Lactamase-Producing Urine Isolates in Istanbul with the Use of Matrix-Assisted Laser Desorption\/Ionization Time-of-Flight Mass-Spectrometry and Real-Time PCR"],"prefix":"10.1177","volume":"23","author":[{"given":"Elif","family":"Akta\u015f","sequence":"first","affiliation":[{"name":"University of Health Sciences","place":["Turkey"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bar\u0131\u015f","family":"Otlu","sequence":"additional","affiliation":[{"name":"Inonu University Faculty of Medicine","place":["Turkey"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duygu","family":"Erdemir","sequence":"additional","affiliation":[{"name":"University of Health Sciences","place":["Turkey"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hatice","family":"Ekici","sequence":"additional","affiliation":[{"name":"University of Health Sciences","place":["Turkey"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emin","family":"Bulut","sequence":"additional","affiliation":[{"name":"University of Health Sciences","place":["Turkey"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2017,5,3]]},"reference":[{"key":"e_1_3_3_2_2","unstructured":"WHO. 2014. Antimicrobial Resistance: Global Report on Surveillance 2014. Available at www.who.int\/drugresistance\/documents\/surveillancereport\/en (accessed December6 2016)."},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2014.0080"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2011.00268.x"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2007.01786.x"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/bs.aambs.2014.09.002"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp194"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2015.07.008"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03196-13"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2014.12.022"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.5578\/mb.6383"},{"key":"e_1_3_3_12_2","first-page":"PI61","article-title":"Prevalence and antimicrobial susceptibility of Escherichia coli in outpatient urinary isolates in Izmir, Turkey","volume":"15","author":"Yilmaz N.","year":"2009","unstructured":"Yilmaz N., , Agus N., , Yurtsever S.G., , Pullukcu H., , Gulay Z., , Coskuner A., , Kose S., , Aydemir S., , Gulenc N., , and Ozgenc O.. 2009. Prevalence and antimicrobial susceptibility of Escherichia coli in outpatient urinary isolates in Izmir, Turkey. Med. Sci. Monit., 15:PI61\u2013PI65.","journal-title":"Med. Sci. Monit."},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1179\/joc.2009.21.4.383"},{"key":"e_1_3_3_14_2","unstructured":"WHO. 2016. Central Asian and Eastern European Surveillance of Antimicrobial Resistance. Annual Report 2016. Available at www.euro.who.int\/en\/health-topics\/disease-prevention\/antimicrobial-resistance\/publications\/2016\/central-asian-and-eastern-european-surveillance-of-antimicrobial-resistance.-annual-report-2016 (accessed December6 2016)."},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2004.00723.x"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01974-07"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2009.02941.x"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkn181"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciu864"},{"key":"e_1_3_3_20_2","first-page":"M100","article-title":"Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fifth Informational Supplement","author":"Clinical and Laboratory Standards Institute","year":"2015","unstructured":"Clinical and Laboratory Standards Institute. 2015. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fifth Informational Supplement. Clinical and Laboratory Standards Institute. M100-S25.","journal-title":"Clinical and Laboratory Standards Institute"},{"key":"e_1_3_3_21_2","unstructured":"The EUCAST guideline on detection of resistance mechanisms v 1.0 (2013-12-11). Available at www.eucast.org\/fileadmin\/src\/media\/PDFs\/EUCAST_files\/Resistance_mechanisms\/EUCAST_detection_of_resistance_mechanisms_v1.0_20131211.pdf (Online)."},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00171-08"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki412"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01377-06"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkh424"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm464"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1402.070350"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02824-14"},{"key":"e_1_3_3_29_2","first-page":"126","article-title":"The antibiotic susceptibilities of E. coli strains producing extended spectrum betalactamases","volume":"33","author":"Yavuzdemir \u015e.","year":"2003","unstructured":"Yavuzdemir \u015e., , Aysev A.D., , and G\u00fcriz H.. 2003. The antibiotic susceptibilities of E. coli strains producing extended spectrum betalactamases. T\u00fcrk. Mikrobiyol. Cem. Derg., 33:126\u2013129.","journal-title":"T\u00fcrk. Mikrobiyol. Cem. Derg."},{"key":"e_1_3_3_30_2","first-page":"88","article-title":"Detection of CTX-M enzymes in ESBL (Extended Spectrum Beta-Lactamases) producing Escherichia coli and Klebsiella spp. isolates","volume":"46","author":"\u00d6gedey E.D.","year":"2016","unstructured":"\u00d6gedey E.D., , C\u00f6mert F., , K\u00f6kt\u00fcrk F., , K\u00fclah C., , and Akta\u015f E.. 2016. Detection of CTX-M enzymes in ESBL (Extended Spectrum Beta-Lactamases) producing Escherichia coli and Klebsiella spp. isolates. T\u00fcrk. Mikrobiyol. Cem. Derg., 46:88\u201396.","journal-title":"T\u00fcrk. Mikrobiyol. Cem. Derg."},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkr553"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2015.10.006"}],"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/mdr.2017.0021","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2017.0021","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2017.0021","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:31:34Z","timestamp":1777667494000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1089\/mdr.2017.0021"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,5,3]]},"references-count":31,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2017,12,1]]}},"alternative-id":["10.1089\/mdr.2017.0021"],"URL":"https:\/\/doi.org\/10.1089\/mdr.2017.0021","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,5,3]]}}}