{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T06:05:35Z","timestamp":1780380335124,"version":"3.54.1"},"reference-count":44,"publisher":"SAGE Publications","issue":"7","license":[{"start":{"date-parts":[[2017,6,26]],"date-time":"2017-06-26T00:00:00Z","timestamp":1498435200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2017,10,1]]},"abstract":"<jats:p>\n                    The nosocomial prevalence of methicillin-resistant\n                    <jats:italic toggle=\"yes\">Staphylococcus aureus<\/jats:italic>\n                    (MRSA) in Portugal is close to 50% and remains one of the highest in Europe. MRSA reservoirs in the animal setting in Portugal have been very poorly investigated, namely among animal husbandry. A total of 52 samples (nasal, inguinal region, and milk) were obtained from bovine animals and analyzed for the presence of\n                    <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                    . The isolates were characterized by pulsed-field gel electrophoresis (PFGE),\n                    <jats:italic toggle=\"yes\">spa<\/jats:italic>\n                    typing, SCC\n                    <jats:italic toggle=\"yes\">mec<\/jats:italic>\n                    typing, and multilocus sequence typing and tested for antimicrobial susceptibility, presence of\n                    <jats:italic toggle=\"yes\">mecA<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">mecC<\/jats:italic>\n                    genes, and virulence determinants. Overall, 54% of the screened animals were colonized with\n                    <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                    in at least one body site. Notably,\n                    <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                    nasal carriage followed an increasing trend with animal age (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009=\u20090.0006). None of the isolates harbored the\n                    <jats:italic toggle=\"yes\">mecA<\/jats:italic>\n                    or\n                    <jats:italic toggle=\"yes\">mecC<\/jats:italic>\n                    genes. Resistance to penicillin, rifampicin, and tetracycline was observed in 24%, 18%, and 6% of the isolates, respectively. The isolates were distributed into three clonal lineages: PFGE type A,\n                    <jats:italic toggle=\"yes\">spa<\/jats:italic>\n                    type t1166, ST1247-CC133 (43%), PFGE B-t267-ST352-CC97 (30%), and PFGE C-t091-ST7-CC7 (27%). CC133 was associated to older animals (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009=\u20090.0025), whereas CC97 was isolated from calves (\n                    <jats:italic toggle=\"yes\">p<\/jats:italic>\n                    \u2009=\u20090.0016). Virulence determinants commonly found in mastitis were widely detected in carriage isolates:\n                    <jats:italic toggle=\"yes\">lukDE<\/jats:italic>\n                    and\n                    <jats:italic toggle=\"yes\">hlgv<\/jats:italic>\n                    (100%),\n                    <jats:italic toggle=\"yes\">hlb<\/jats:italic>\n                    (76%), and\n                    <jats:italic toggle=\"yes\">lukM<\/jats:italic>\n                    (35%). Although healthy bovines do not represent a MRSA reservoir in Portugal, they are mainly colonized with\n                    <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                    pathogenic lineages associated to mastitis in cattle (CC97 and CC133).\n                  <\/jats:p>","DOI":"10.1089\/mdr.2017.0074","type":"journal-article","created":{"date-parts":[[2017,6,26]],"date-time":"2017-06-26T13:53:30Z","timestamp":1498485210000},"page":"845-851","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":15,"title":["Healthy Bovines as Reservoirs of Major Pathogenic Lineages of\n                    <i>Staphylococcus aureus<\/i>\n                    in Portugal"],"prefix":"10.1177","volume":"23","author":[{"given":"Teresa","family":"Concei\u00e7\u00e3o","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Genetics, Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, Oeiras, Portugal."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herm\u00ednia","family":"de Lencastre","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Genetics, Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, Oeiras, Portugal."},{"name":"The Rockefeller University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Aires-de-Sousa","sequence":"additional","affiliation":[{"name":"Escola Superior de Sa\u00fade da Cruz Vermelha Portuguesa, Lisboa, Portugal."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2017,6,26]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0450.1972.tb00439.x"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1080\/01652176.2007.9695224"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2014.12.007"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2012.01.006"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2011.09.004"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2016.01.021"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40588-016-0031-9"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0010990"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2012.06.006"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1186\/1746-6148-10-153"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.5812\/jjm.22413"},{"key":"e_1_3_3_13_2","volume-title":"Annual Report of the European Antimicrobial Resistance Surveillance Network (EARS-Net)","author":"ECDC","year":"2017","unstructured":"ECDC. 2017. 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