{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T13:01:02Z","timestamp":1790686862814,"version":"4.1.0"},"reference-count":15,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2018,4,1]],"date-time":"2018-04-01T00:00:00Z","timestamp":1522540800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2018,4]]},"abstract":"\n Aims:<\/jats:title>\n \n To identify plasmid-mediated colistin resistance in clinical\n Enterobacteriaceae<\/jats:italic>\n isolates in Oman, where this resistance mechanism has not been encountered yet.\n <\/jats:p>\n <\/jats:sec>\n \n Materials\/Methods:<\/jats:title>\n \n Twenty-two colistin-resistant\n Enterobacteriaceae<\/jats:italic>\n clinical isolates collected between July 2014 and June 2016 in a tertiary care hospital in Muscat were screened by PCR for the\n mcr-1<\/jats:italic>\n and\n mcr-2<\/jats:italic>\n genes. The strain identified as\n mcr-1<\/jats:italic>\n positive was genotyped and its antibiotic susceptibility was established. The\n mcr-1<\/jats:italic>\n containing plasmid was mobilized into\n Escherichia coli<\/jats:italic>\n K-12 and its sequence was determined.\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n A single\n E. coli<\/jats:italic>\n isolate (OM97) carrying\n mcr-1<\/jats:italic>\n gene was identified, while no strains carrying the\n mcr-2<\/jats:italic>\n gene was found.\n E. coli<\/jats:italic>\n OM97 was isolated in June 2016 from blood culture of a male patient with multiple comorbidities. It belonged to ST10. Beyond colistin, it was resistant to amoxicillin\u2013clavulanic acid, piperacillin\u2013tazobactam, amikacin, ciprofloxacin, tetracycline, and cotrimoxazole. The\n mcr-1<\/jats:italic>\n gene was located on a conjugative IncI2-type plasmid of 63722\u2009bp size, which did not harbor any further resistance genes. The genetic surrounding of the\n mcr-1<\/jats:italic>\n gene lacked the IS\n Apl1<\/jats:italic>\n element.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions:<\/jats:title>\n \n Although colistin resistance caused by the\n mcr-1<\/jats:italic>\n gene is not common in our collection of clinical isolates, the occurrence of the plasmid-mediated colistin resistance in an\n E. coli<\/jats:italic>\n ST10 strain is of concern as this clonal group was already shown to spread ESBL genes and quinolone resistance worldwide. It is especially worrisome that as the\n mcr-1<\/jats:italic>\n gene occurred in a non-ESBL, carbapenem-susceptible\n E. coli<\/jats:italic>\n strain, current susceptibility testing algorithms may not detect its presence.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1089\/mdr.2017.0131","type":"journal-article","created":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T09:32:25Z","timestamp":1502443945000},"page":"278-282","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":27,"title":["Plasmid-Mediated Colistin Resistance Gene\n mcr-1<\/i>\n in an\n Escherichia coli<\/i>\n ST10 Bloodstream Isolate in the Sultanate of Oman"],"prefix":"10.1177","volume":"24","author":[{"given":"Jalila","family":"Mohsin","sequence":"first","affiliation":[{"name":"The Royal Hospital","place":["Oman"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tibor","family":"P\u00e1l","sequence":"additional","affiliation":[{"name":"United Arab Emirates University","place":["United Arab Emirates"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorgen Eskild","family":"Petersen","sequence":"additional","affiliation":[{"name":"The Royal Hospital","place":["Oman"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dania","family":"Darwish","sequence":"additional","affiliation":[{"name":"United Arab Emirates University","place":["United Arab Emirates"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akela","family":"Ghazawi","sequence":"additional","affiliation":[{"name":"United Arab Emirates University","place":["United Arab Emirates"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanveer","family":"Ashraf","sequence":"additional","affiliation":[{"name":"United Arab Emirates University","place":["United Arab Emirates"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnes","family":"Sonnevend","sequence":"additional","affiliation":[{"name":"United Arab Emirates University","place":["United Arab Emirates"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2018,4]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(15)00424-7"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00064-16"},{"key":"e_1_3_3_4_2","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.ijid.2016.07.007","article-title":"Plasmid-mediated colistin resistance in Escherichia coli from the Arabian Peninsula","volume":"50","author":"Sonnevend A.","year":"2016","unstructured":"Sonnevend A., , Ghazawi A., , Alqahtani M., , Shibl A., , Jamal W., , Hashmey R., , and Pal T.. 2016. Plasmid-mediated colistin resistance in Escherichia coli from the Arabian Peninsula. Int J Infect Dis., 50:85\u201390.","journal-title":"Int J Infect Dis."},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01486-16"},{"key":"e_1_3_3_6_2","doi-asserted-by":"crossref","DOI":"10.2807\/1560-7917.ES.2016.21.27.30280","article-title":"Identification of a novel plasmid-mediated colistin-resistance gene, mcr-2, in Escherichia coli, Belgium, June 2016","volume":"21","author":"Xavier B.B.","year":"2016","unstructured":"Xavier B.B., , Lammens C., , Ruhal R., , Kumar-Singh S., , Butaye P., , Goossens H., , and Malhotra-Kumar S.. 2016. Identification of a novel plasmid-mediated colistin-resistance gene, mcr-2, in Escherichia coli, Belgium, June 2016. Euro Surveill, 2016; 21. DOI: 10.2807\/1560-7917.ES.2016.21.27.30280.","journal-title":"Euro Surveill"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05172.x"},{"key":"e_1_3_3_8_2","unstructured":"EUCAST Breakpoint tables for interpretation of MICs and zone diameters 2016. Available at www.eucast.org (Accessed December15 2016)."},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02412-14"},{"key":"e_1_3_3_10_2","first-page":"2017","article-title":"Prevalence of mcr-1 in Escherichia coli and Klebsiella pneumoniae recovered from bloodstream infections in China: a multicentre longitudinal study","author":"Quan J.","year":"2017","unstructured":"Quan J., , Li X., , Chen Y., , Jiang Y., , Zhou Z., , Zhang H., , Sun L., , Ruan Z., , Feng Y., , Akova M., , and Yu Y.. 2017. Prevalence of mcr-1 in Escherichia coli and Klebsiella pneumoniae recovered from bloodstream infections in China: a multicentre longitudinal study. Lancet Infect Dis. 2017.","journal-title":"Lancet Infect Dis."},{"key":"e_1_3_3_11_2","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1093\/dnares\/dsu043","article-title":"A complete view of the genetic diversity of the Escherichia coli O-antigen biosynthesis gene cluster","volume":"22","author":"Itsushi A.","year":"2015","unstructured":"Itsushi A., , Iyoda S., , Kikuchi T., , Ogura Y., , Katsura K., , Ohnishi M., , Hayashi T., , and Thomson N.R.. 2015. A complete view of the genetic diversity of the Escherichia coli O-antigen biosynthesis gene cluster. DNA Res., 22:101\u2013107.","journal-title":"DNA Res."},{"key":"e_1_3_3_12_2","first-page":"2017","article-title":"Prevalence, risk factors, outcomes, and molecular epidemiology of mcr-1-positive Enterobacteriaceae in patients and healthy adults from China: an epidemiological and clinical study","author":"Wang Y.","year":"2017","unstructured":"Wang Y., , Tian G.B., , Zhang R., , Shen Y., , Tyrrell J.M., , Huang X., , Zhou H., , Lei L., , Li H.Y., , Doi Y., , Fang Y., , Ren H., , Zhong L.L., , Shen Z., , Zeng K.J., , Wang S., , Liu J.H., , Wu C., , Walsh T.R., , and Shen J.. 2017. Prevalence, risk factors, outcomes, and molecular epidemiology of mcr-1-positive Enterobacteriaceae in patients and healthy adults from China: an epidemiological and clinical study. Lancet Infect Dis. 2017.","journal-title":"Lancet Infect Dis."},{"key":"e_1_3_3_13_2","doi-asserted-by":"crossref","first-page":"5080","DOI":"10.1128\/AAC.00731-16","article-title":"Travelers can import colistin-resistant Enterobacteriaceae, including those possessing the plasmid-mediated mcr-1 gene","volume":"60","author":"Bernasconi O.J.","year":"2016","unstructured":"Bernasconi O.J., , Kuenzli E., , Pires J., , Tinguely R., , Carattoli A., , Hatz C., , Perreten V., , and Endiminani A.. 2016. Travelers can import colistin-resistant Enterobacteriaceae, including those possessing the plasmid-mediated mcr-1 gene. Antimicrob Agents Chemother., 60:5080\u20135084.","journal-title":"Antimicrob Agents Chemother."},{"key":"e_1_3_3_14_2","first-page":"2017","article-title":"Environmental emission of multiresistant Escherichia coli carrying the colistin resistance gene mcr-1 from German swine farms","author":"Guenther S.","year":"2017","unstructured":"Guenther S., , Falgenhauer L., , Semmler T., , Imirzalioglu C., , Chakraborty T., , Roesler U., , and Roschanski N.. 2017. Environmental emission of multiresistant Escherichia coli carrying the colistin resistance gene mcr-1 from German swine farms. J Antimicrob Chemother. 2017.","journal-title":"J Antimicrob Chemother."},{"key":"e_1_3_3_15_2","first-page":"e1","article-title":"mcr-1 is borne by highly diverse Escherichia coli isolates since 2004 in food-producing animals in Europe","author":"El Garch F.","year":"2017","unstructured":"El Garch F., , Sauget M., , Hocquet D., , LeChaudee D., , Woehrle F., , and Bertrand X.. 2017. mcr-1 is borne by highly diverse Escherichia coli isolates since 2004 in food-producing animals in Europe. Clin Microbiol Infect. 23:51. e1\u2013e4.","journal-title":"Clin Microbiol Infect. 23:51"},{"key":"e_1_3_3_16_2","first-page":"e02569","article-title":"Occurrence of blaKPC-2, blaCTX-M and mcr-1 in Enterobacteriaceae from Well Water in rural China","volume":"61","author":"Sun P.","year":"2017","unstructured":"Sun P., , Bi Z., , Nilsson M., , Zheng B., , Berglund B., , Stalsby Lundborg C., , B\u00f6rjesson S., , Li X., , Chen B., , Yin H., , and Nilsson L.E.. 2017. Occurrence of blaKPC-2, blaCTX-M and mcr-1 in Enterobacteriaceae from Well Water in rural China. Antimicrob Agents Chemother., 61:e02569\u2013e16.","journal-title":"Antimicrob Agents Chemother."}],"container-title":["Microbial Drug Resistance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1089\/mdr.2017.0131","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2017.0131","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1089\/mdr.2017.0131","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:31:56Z","timestamp":1777667516000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/full\/10.1089\/mdr.2017.0131"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,4]]},"references-count":15,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2018,4]]}},"alternative-id":["10.1089\/mdr.2017.0131"],"URL":"https:\/\/doi.org\/10.1089\/mdr.2017.0131","relation":{},"ISSN":["1076-6294","1931-8448"],"issn-type":[{"value":"1076-6294","type":"print"},{"value":"1931-8448","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,4]]}}}