{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T07:28:37Z","timestamp":1789543717459,"version":"build-2803163510"},"reference-count":32,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2018,4,1]],"date-time":"2018-04-01T00:00:00Z","timestamp":1522540800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:p>\n                    A plasmid pCY-CTX carrying a phage-like backbone from an extensively drug-resistant\n                    <jats:italic toggle=\"yes\">Enterobacter cloacae<\/jats:italic>\n                    strain Guangzhou-ECL001 (previously known as CY01) was identified in this study. By Illumina MiSeq 2\u2009\u00d7\u2009250-bp paired-end sequencing,\n                    <jats:italic toggle=\"yes\">de novo<\/jats:italic>\n                    assembly, and PCR, full sequence of pCY-CTX was obtained. Plasmid pCY-CTX was a circular plasmid with a length of 116,700\u2009bp, harboring 136 putative open reading frames with the average G\u2009+\u2009C content of 50.8%. The backbone of pCY-CTX showed high identity to previously reported phage-like plasmid pHCM2 and phage SSU5. In addition, pCY-CTX contained a distinctive IS\n                    <jats:italic toggle=\"yes\">Ecp1<\/jats:italic>\n                    -mediated Tn\n                    <jats:italic toggle=\"yes\">2<\/jats:italic>\n                    region with two resistance genes\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>TEM-1<\/jats:sub>\n                    and\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>CTX-M-3<\/jats:sub>\n                    . Transposition unit \u201cIS\n                    <jats:italic toggle=\"yes\">Ecp1- bla<\/jats:italic>\n                    <jats:sub>CTX-M-3<\/jats:sub>\n                    <jats:italic toggle=\"yes\">- orf477<\/jats:italic>\n                    \u201d was inserted into the Tn\n                    <jats:italic toggle=\"yes\">2<\/jats:italic>\n                    structure, dividing Tn\n                    <jats:italic toggle=\"yes\">2<\/jats:italic>\n                    into two parts. This represents the first identification of a plasmid carrying a phage-like backbone and a distinctive IS\n                    <jats:italic toggle=\"yes\">Ecp1<\/jats:italic>\n                    -mediated Tn\n                    <jats:italic toggle=\"yes\">2<\/jats:italic>\n                    region within\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>TEM-1<\/jats:sub>\n                    and\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>CTX-M-3<\/jats:sub>\n                    in clinical\n                    <jats:italic toggle=\"yes\">E. cloacae<\/jats:italic>\n                    . The finding of phage-like regions located in plasmids provides a new perspective in gene transfer associated with antimicrobial resistance.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2017.0146","type":"journal-article","created":{"date-parts":[[2017,9,6]],"date-time":"2017-09-06T09:54:56Z","timestamp":1504691696000},"page":"307-313","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":17,"title":["Complete Sequence of pCY-CTX, a Plasmid Carrying a Phage-Like Region and an IS\n                    <i>Ecp1<\/i>\n                    -Mediated Tn\n                    <i>2<\/i>\n                    Element from\n                    <i>Enterobacter cloacae<\/i>"],"prefix":"10.1177","volume":"24","author":[{"given":"Zhenbo","family":"Xu","sequence":"first","affiliation":[{"name":"South China University of Technology","place":["China"]},{"name":"University of Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinhong","family":"Xie","sequence":"additional","affiliation":[{"name":"South China University of Technology","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Yang","sequence":"additional","affiliation":[{"name":"First Affiliated Hospital of Guangzhou Medical University","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dingqiang","family":"Chen","sequence":"additional","affiliation":[{"name":"First Affiliated Hospital of Guangzhou Medical University","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian M.","family":"Peters","sequence":"additional","affiliation":[{"name":"University of Tennessee Health Sciences Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark E.","family":"Shirtliff","sequence":"additional","affiliation":[{"name":"University of Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2018,4]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.12.61"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01641355"},{"key":"e_1_3_4_4_2","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1080\/10408410490266429","article-title":"Extended-spectrum \u03b2-lactamases (ESBLs): characterization, epidemiology and detection","volume":"30","author":"Shah A.A.","year":"2004","unstructured":"Shah A.A., , Hasan F., , Ahmed S., , and Hameed A., 2004. 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