{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:27:58Z","timestamp":1777922878261,"version":"3.51.4"},"reference-count":74,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2018,7,1]],"date-time":"2018-07-01T00:00:00Z","timestamp":1530403200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2018,7]]},"abstract":"<jats:p>\n                    Alterations in PBP2a have been recognized in cefotaxime-resistant laboratory mutants and \u03b2-lactam\u2013resistant clinical isolates of\n                    <jats:italic toggle=\"yes\">Streptococcus pneumoniae<\/jats:italic>\n                    . DNA sequencing revealed fundamental differences between these two settings. Internal stop codons in\n                    <jats:italic toggle=\"yes\">pbp2a<\/jats:italic>\n                    occurred in all three laboratory mutants analyzed, caused by a mutation in\n                    <jats:italic toggle=\"yes\">pbp2a<\/jats:italic>\n                    of mutant C604, and tandem duplications within\n                    <jats:italic toggle=\"yes\">pbp2a<\/jats:italic>\n                    resulting in premature stop codons in another two mutants C403 and C406. In contrast, mosaic PBP2a genes were observed in several penicillin-resistant clinical isolates from South Africa, the Czech Republic, Hungary, and in the clone Poland\n                    <jats:sup>23F<\/jats:sup>\n                    -16, with sequence blocks diverging from sensitive strains by over 4%. Most of these\n                    <jats:italic toggle=\"yes\">pbp2a<\/jats:italic>\n                    variants except\n                    <jats:italic toggle=\"yes\">pbp2a<\/jats:italic>\n                    from the South African strain contained sequences related to\n                    <jats:italic toggle=\"yes\">pbp2a<\/jats:italic>\n                    of\n                    <jats:italic toggle=\"yes\">Streptococcus mitis<\/jats:italic>\n                    B6, confirming that this species serves as reservoir for penicillin-resistance determinants.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2017.0302","type":"journal-article","created":{"date-parts":[[2017,12,1]],"date-time":"2017-12-01T17:07:24Z","timestamp":1512148044000},"page":"718-731","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["PBP2a in \u03b2-Lactam\u2013Resistant Laboratory Mutants and Clinical Isolates: Disruption Versus Reduced Penicillin Affinity"],"prefix":"10.1177","volume":"24","author":[{"given":"Mark","family":"van der Linden","sequence":"first","affiliation":[{"name":"German National Reference Center for Streptococci","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jens","family":"Rutschmann","sequence":"additional","affiliation":[{"name":"BA.Impfstoffe GmbH","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick","family":"Maurer","sequence":"additional","affiliation":[{"name":"University of Applied Sciences","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Regine","family":"Hakenbeck","sequence":"additional","affiliation":[{"name":"University of Kaiserslautern","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2018,7]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1097\/00000441-196509000-00003","article-title":"Susceptibility of pneumococci to nine antibiotics","volume":"250","author":"Kislak J.W.","year":"1965","unstructured":"Kislak J.W., , Razavi L.M., , Daly A.K., , and Finland M.. 1965. 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