{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:28:01Z","timestamp":1777922881952,"version":"3.51.4"},"reference-count":52,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2019,3,1]],"date-time":"2019-03-01T00:00:00Z","timestamp":1551398400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2019,3,1]]},"abstract":"<jats:p>\n                    The family\n                    <jats:italic toggle=\"yes\">Legionellaceae<\/jats:italic>\n                    consists of Gram-negative bacteria that are widely distributed in aquatic environments around the world. This family consists of a single genus,\n                    <jats:italic toggle=\"yes\">Legionella<\/jats:italic>\n                    , that is recognized as an important cause of community-acquired pneumonia and hospital-acquired pneumonia.\n                    <jats:italic toggle=\"yes\">Legionella<\/jats:italic>\n                    consists of intracellular pathogens, thus cellular pharmacokinetic and pharmacodynamic properties of an antibiotic against these bacteria as well as uptake and subcellular distribution into macrophages should be considered for a successful outcome of disease. Treatment strategies for\n                    <jats:italic toggle=\"yes\">Legionella<\/jats:italic>\n                    infection require a combination of multiple antibiotics. Hence, because of the possible development of resistance to the drugs during therapy, a new alternative targeted therapy is yielding promising results. In this study, a comprehensive\n                    <jats:italic toggle=\"yes\">in silico<\/jats:italic>\n                    target identification pipeline was performed on members of the family\n                    <jats:italic toggle=\"yes\">Legionellaceae<\/jats:italic>\n                    to identify the best targets. Using a homology-based computational pipeline method, new drug targets were identified. Of 4,358 analyzed proteins, 18 proteins, including proteins involved in metabolism (amino acid, energy, and lipid metabolisms), cellular transport, cell division, and cell motility, were selected as the final putative drug targets. These proteins play an important role in the survival and propagation of\n                    <jats:italic toggle=\"yes\">Legionella<\/jats:italic>\n                    infection. In conclusion, homology-based methods could improve the identification of novel drug targets and the drug discovery process, which can potentially be effective for the prevention and treatment of\n                    <jats:italic toggle=\"yes\">Legionella<\/jats:italic>\n                    infections.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2017.0328","type":"journal-article","created":{"date-parts":[[2018,8,2]],"date-time":"2018-08-02T14:17:52Z","timestamp":1533219472000},"page":"157-166","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["A Systematic\n                    <i>In Silico<\/i>\n                    Analysis of the\n                    <i>Legionellaceae<\/i>\n                    Family for Identification of Novel Drug Target Candidates"],"prefix":"10.1177","volume":"25","author":[{"given":"Seyyed Mohsen","family":"Sohrabi","sequence":"first","affiliation":[{"name":"Lorestan University","place":["Iran"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohsen","family":"Mohammadi","sequence":"additional","affiliation":[{"name":"Lorestan University of Medical Sciences","place":["Iran"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seyyede Nasim","family":"Tabatabaiepour","sequence":"additional","affiliation":[{"name":"Shahrekord University","place":["Iran"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seyyede Zahra","family":"Tabatabaiepour","sequence":"additional","affiliation":[{"name":"Ilam University","place":["Iran"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hossein","family":"Hosseini-Nave","sequence":"additional","affiliation":[{"name":"Kerman University of Medical Sciences","place":["Iran"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad Fazel","family":"Soltani","sequence":"additional","affiliation":[{"name":"Razi University","place":["Iran"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hosniyeh","family":"Alizadeh","sequence":"additional","affiliation":[{"name":"Kerman University of Medical Sciences","place":["Iran"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Morteza","family":"Hadizadeh","sequence":"additional","affiliation":[{"name":"Kerman University of Medical Sciences","place":["Iran"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2019,3,11]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00005-009-0035-8"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2009.05.004"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11684-010-0036-3"},{"key":"e_1_3_3_5_2","unstructured":"Bartram J. 2007. 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