{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T17:44:27Z","timestamp":1778175867253,"version":"3.51.4"},"reference-count":29,"publisher":"SAGE Publications","issue":"10","license":[{"start":{"date-parts":[[2018,12,1]],"date-time":"2018-12-01T00:00:00Z","timestamp":1543622400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2018,12,1]]},"abstract":"<jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>\n                      The aim of this study was to characterize the O25b\/ST131 clone in ciprofloxacin-resistant\n                      <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                      isolates from Yemen.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>\n                      A total of 41 ciprofloxacin-resistant\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      strains were collected from clinical samples of inpatients and outpatients from Sana'a (Yemen) from January to December 2013. Antimicrobial susceptibility testing, polymerase chain reaction amplification, and sequencing were used for detection of plasmid-mediated quinolone resistance determinants, extended-spectrum beta-lactamases genes and mutations in the quinolone resistance-determining regions of the target genes\n                      <jats:italic toggle=\"yes\">gyrA<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">parC<\/jats:italic>\n                      . Genetic relatedness of\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      isolates was determined by pulsed-field gel electrophoresis (PFGE). O25b\/ST131 clone detection was performed using polymerase chain reaction of O25b\n                      <jats:italic toggle=\"yes\">rfb<\/jats:italic>\n                      and allele 3 of the\n                      <jats:italic toggle=\"yes\">pabB<\/jats:italic>\n                      gene and by a multilocus sequence typing.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      All\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      isolates contained the\n                      <jats:italic toggle=\"yes\">aac(6<\/jats:italic>\n                      \u2032\n                      <jats:italic toggle=\"yes\">)Ib-c<\/jats:italic>\n                      r gene associated with\n                      <jats:italic toggle=\"yes\">\n                        bla\n                        <jats:sub>CTX-M-15<\/jats:sub>\n                      <\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">qnrS<\/jats:italic>\n                      genes in 63.4% and 12.2%, respectively. A rate of 36.6% (15\/41) of O25b\/ST131\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      isolates were identified belonging to the H30-Rx subclone producing both CTX-M-15 and Aac(6\u2032)Ib-cr enzymes and carrying two substitutions in GyrA (Ser83Leu\/Asp87Asn) and two substitutions in ParC (Ser80Ile\/Glu84Val). Most of them were uropathogenic unrelated\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      isolates recovered from outpatients.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>\n                      This is the first report of a high prevalence of\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      O25b\/ST131 from Yemen.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1089\/mdr.2018.0024","type":"journal-article","created":{"date-parts":[[2018,6,8]],"date-time":"2018-06-08T16:00:47Z","timestamp":1528473647000},"page":"1537-1542","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":10,"title":["Prevalence of ST131 Clone Producing Both ESBL CTX-M-15 and AAC(6\u2032)Ib-cr Among Ciprofloxacin-Resistant\n                    <i>Escherichia coli<\/i>\n                    Isolates from Yemen"],"prefix":"10.1177","volume":"24","author":[{"given":"Sameer Ahmed","family":"Alsharapy","sequence":"first","affiliation":[{"name":"Universit\u00e9 de Bejaia","place":["Algeria"]},{"name":"National Center of Public Health Laboratories (NCPHL)","place":["Yemen"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Betitera","family":"Yanat","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Bejaia","place":["Algeria"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorena","family":"Lopez-Cerero","sequence":"additional","affiliation":[{"name":"Hospital Universitario Virgen Macarena","place":["Spain"]},{"name":"Universidad de Sevilla","place":["Spain"]},{"name":"Instituto de Biomedicina de Sevilla (IBiS)","place":["Spain"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samar Saeed","family":"Nasher","sequence":"additional","affiliation":[{"name":"National Center of Public Health Laboratories (NCPHL)","place":["Yemen"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paula","family":"D\u00edaz-De-Alba","sequence":"additional","affiliation":[{"name":"Hospital Universitario Virgen Macarena","place":["Spain"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c1lvaro","family":"Pascual","sequence":"additional","affiliation":[{"name":"Hospital Universitario Virgen Macarena","place":["Spain"]},{"name":"Universidad de Sevilla","place":["Spain"]},{"name":"Instituto de Biomedicina de Sevilla (IBiS)","place":["Spain"]},{"name":"Instituto de Salud Carlos III","place":["Spain"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9-Manuel","family":"Rodr\u00edguez-Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Universidad de Sevilla","place":["Spain"]},{"name":"Instituto de Biomedicina de Sevilla (IBiS)","place":["Spain"]},{"name":"Instituto de Salud Carlos III","place":["Spain"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdelaziz","family":"Touati","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Bejaia","place":["Algeria"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2018,12,8]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro818"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2012.00009"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/bs.aambs.2014.09.002"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00125-13"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1086\/653932"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkq415"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkt405"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01025-12"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.7.2161-2168.2003"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jis933"},{"key":"e_1_3_3_12_2","doi-asserted-by":"crossref","unstructured":"Pitout J.D. and DeVinney R.. 2017. 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