{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:28:35Z","timestamp":1777922915202,"version":"3.51.4"},"reference-count":34,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2019,7,1]],"date-time":"2019-07-01T00:00:00Z","timestamp":1561939200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:p>\n                    The aim of this study was to investigate the frequency, antimicrobial sensitivity profile, and genetic characteristics of nosocomial strains of extended-spectrum \u03b2-lactamase (ESBL)-producing\n                    <jats:italic toggle=\"yes\">Klebsiella pneumoniae<\/jats:italic>\n                    isolated from inpatients at a teaching hospital in Brazil. The bacterial identification, phenotypic detection of ESBL, and antimicrobial susceptibility profile were performed by the VITEK 2 automated system. Matrix-assisted laser desorption\/ionization-time of flight mass spectrometry (MALDI-TOF MS) mass spectrometry was used to confirm the identity of the species and genotyping of ESBL-producing\n                    <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                    was performed by pulsed-field gel electrophoresis (PFGE). Thirty-six ESBL-producing\n                    <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                    nosocomial strains isolated from November 2013 to August 2014 were analyzed. High resistance rates were observed for ceftriaxone, ceftazidime, cefepime, gentamicin, and ciprofloxacin. However, all isolates were susceptible to amikacin and meropenem. All strains harbored\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>CTX-M-like<\/jats:sub>\n                    and\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>SHV-like<\/jats:sub>\n                    genes. Molecular typing by PFGE showed a diversity of genotypes distributed among 25 clusters, but two isolates collected in different wards had the same genotypic profile and carried the same\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    genes, so they were considered clones. The data showed that there was a high frequency of ESBL-producing\n                    <jats:italic toggle=\"yes\">K. pneumoniae<\/jats:italic>\n                    multidrug-resistant among patients in the studied hospital. Furthermore, the detection of\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>CTX-M-like<\/jats:sub>\n                    genes in all isolates suggests that these enzymes are the major ESBL responsible for the beta-lactam resistance phenotypes among the analyzed strains.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2018.0142","type":"journal-article","created":{"date-parts":[[2019,2,27]],"date-time":"2019-02-27T10:52:45Z","timestamp":1551264765000},"page":"909-914","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["High Frequency of Extended-Spectrum Beta-Lactamase-Producing\n                    <i>Klebsiella pneumoniae<\/i>\n                    Nosocomial Strains Isolated from a Teaching Hospital in Brazil"],"prefix":"10.1177","volume":"25","author":[{"given":"Francisco Ruligl\u00e9sio","family":"Rocha","sequence":"first","affiliation":[{"name":"Microbiology Laboratory, FAMED, Federal University of Cear\u00e1, Sobral, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorena Cristina Corr\u00eaa","family":"Fehlberg","sequence":"additional","affiliation":[{"name":"Laboratory Alerta, Division of Infectious Diseases, Federal University of S\u00e3o Paulo, S\u00e3o Paulo, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jhonatha Rodrigo","family":"Cordeiro-Moura","sequence":"additional","affiliation":[{"name":"Laboratory Alerta, Division of Infectious Diseases, Federal University of S\u00e3o Paulo, S\u00e3o Paulo, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Carolina","family":"Ramos","sequence":"additional","affiliation":[{"name":"Laboratory Alerta, Division of Infectious Diseases, Federal University of S\u00e3o Paulo, S\u00e3o Paulo, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vicente de Paulo Teixeira","family":"Pinto","sequence":"additional","affiliation":[{"name":"Microbiology Laboratory, FAMED, Federal University of Cear\u00e1, Sobral, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco Cesar Barroso","family":"Barbosa","sequence":"additional","affiliation":[{"name":"Microbiology Laboratory, FAMED, Federal University of Cear\u00e1, Sobral, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2019,7,11]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"crossref","first-page":"1604","DOI":"10.1093\/jac\/dkq190","article-title":"Detection of the new metallo-\u03b2-lactamase VIM-19 along with KPC-2, CMY-2, and CTX-M-15 in Klebsiella pneumoniae","volume":"65","author":"Pournaras S.","year":"2010","unstructured":"Pournaras S., , Poulou A., , Voulgari E., , Vrioni G., , Kristo I., , and Tsakris A.. 2010. 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