{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:28:39Z","timestamp":1777922919805,"version":"3.51.4"},"reference-count":40,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2019,5,30]],"date-time":"2019-05-30T00:00:00Z","timestamp":1559174400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2019,6,1]]},"abstract":"<jats:p>\n                    Thirty-five serotype K1 hypervirulent\n                    <jats:italic toggle=\"yes\">Klebsiella pneumoniae<\/jats:italic>\n                    (K1-hvKP) isolates collected from a Chinese hospital during the whole year of 2017 were evaluated to characterize the prevalence of the plasmid-mediated quinolone resistance (PMQR) genes. In total, 18 (51.4%) isolates were detected to carry PMQR genes, and the most frequently detected gene was\n                    <jats:italic toggle=\"yes\">qnrS1<\/jats:italic>\n                    (37.5%), followed by\n                    <jats:italic toggle=\"yes\">aac(6\u2032)-Ib-cr<\/jats:italic>\n                    (15%) and\n                    <jats:italic toggle=\"yes\">qnrB4<\/jats:italic>\n                    (2.5%). Remarkably, some\n                    <jats:italic toggle=\"yes\">qnr<\/jats:italic>\n                    -carrying strains had only a single or more quinolone resistance-determining region mutations in GyrA or ParC, and most exhibited high-level ciprofloxacin resistance. However, only 11.4% (4\/35) isolates were resistant to quinolones. Furthermore, 34.3% (12\/35) carried extended-spectrum \u03b2-lactamase (ESBL) genes, but only 14.3% (5\/35) exhibited ESBL phenotype. The most prevalent virulence genes were\n                    <jats:italic toggle=\"yes\">mrkD<\/jats:italic>\n                    (100%, 21\/21), followed by\n                    <jats:italic toggle=\"yes\">magA<\/jats:italic>\n                    (97.1%, 34\/35),\n                    <jats:italic toggle=\"yes\">wabG<\/jats:italic>\n                    (97.1%, 34\/35),\n                    <jats:italic toggle=\"yes\">rmpA<\/jats:italic>\n                    (97.1%, 34\/35),\n                    <jats:italic toggle=\"yes\">aerobactin<\/jats:italic>\n                    (94.3%, 33\/35),\n                    <jats:italic toggle=\"yes\">kfuB<\/jats:italic>\n                    (94.3%, 33\/35),\n                    <jats:italic toggle=\"yes\">ycf<\/jats:italic>\n                    (91.4%, 32\/35),\n                    <jats:italic toggle=\"yes\">iutA<\/jats:italic>\n                    (91.4%, 32\/35),\n                    <jats:italic toggle=\"yes\">rmpA2<\/jats:italic>\n                    (62.9%, 22\/35),\n                    <jats:italic toggle=\"yes\">wcaG<\/jats:italic>\n                    (62.9%, 22\/35),\n                    <jats:italic toggle=\"yes\">ybtS<\/jats:italic>\n                    (51.4%, 18\/35),\n                    <jats:italic toggle=\"yes\">allS<\/jats:italic>\n                    (45.7%, 16\/35), and\n                    <jats:italic toggle=\"yes\">iroN<\/jats:italic>\n                    (22.9%, 8\/35). Multilocus sequence typing (MLST) analysis assigned the 35 K1-hvKP isolates into 5 sequence types (STs), with ST23 encompassing 77.1% of the strains. Pulsed field gel electrophoresis (PFGE) typing showed that strains closely related by MLST clustered in major PFGE clusters, of which cluster A accounts for 16 ST23 isolates and cluster B includes 11 ST23 isolates. The analysis of the transconjugants showed decreased susceptibility to quinolones and revealed a cotransfer of\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>CTX-M-15<\/jats:sub>\n                    with the\n                    <jats:italic toggle=\"yes\">qnr<\/jats:italic>\n                    S1 alleles. Cumulatively, our findings showed that the PMQR-producing K1-hvKP strain is covertly spreading even when K1-hvKP is rarely resistant to fluoroquinolones (FQs) according to the Clinical and Laboratory Standards Institute criteria. It is therefore critical to continuously monitor the PMQR-producing K1-hvKP epidemiology and minimize potential risks from FQ-resistant K1-hvKP.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2018.0173","type":"journal-article","created":{"date-parts":[[2019,1,8]],"date-time":"2019-01-08T00:31:27Z","timestamp":1546907487000},"page":"681-689","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":17,"title":["High Prevalence of Plasmid-Mediated Quinolone Resistance Determinants Among Serotype K1 Hypervirulent\n                    <i>Klebsiella pneumoniae<\/i>\n                    Isolates in China"],"prefix":"10.1177","volume":"25","author":[{"given":"Yang","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Bacteriology, First Affiliated Hospital of Nanchang University, Nanchang University, Nanchang, P.R. China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fang-ling","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, First Affiliated Hospital of Nanchang University, Nanchang University, Nanchang, P.R. China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tian-xin","family":"Xiang","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease, First Affiliated Hospital of Nanchang University, Nanchang University, Nanchang, P.R. China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"La-Gen","family":"Wan","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, First Affiliated Hospital of Nanchang University, Nanchang University, Nanchang, P.R. China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan-dan","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, First Affiliated Hospital of Nanchang University, Nanchang University, Nanchang, P.R. China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xian-Wei","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Hospital Infection Control, First Affiliated Hospital of Nanchang University, Nanchang University, Nanchang, P.R. China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Respiration, The First Affiliated Hospital of Nanchang University, Nanchang, P.R. 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