{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T19:28:51Z","timestamp":1777922931549,"version":"3.51.4"},"reference-count":35,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2019,5,30]],"date-time":"2019-05-30T00:00:00Z","timestamp":1559174400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2019,6,1]]},"abstract":"<jats:p>\n                    <jats:italic toggle=\"yes\">Chryseobacterium indologenes<\/jats:italic>\n                    is an emerging nosocomial pathogen that produces IND-type chromosomal metallo-beta-lactamase. The phenotype and molecular aspects of two multidrug resistant\n                    <jats:italic toggle=\"yes\">C. indologenes<\/jats:italic>\n                    strains and the analysis of the tertiary structure of the IND enzyme were studied. Identification of species and susceptibility tests were performed using the Vitek-2 compact. Chromosomal and plasmid DNA were extracted using PureLink\u2122 Genomic DNA Mini Kit and PureLink Quick Plasmid Miniprep Kit, and the sequencing was performed using ABI 3130 genetic analyzer. Two strains were isolated and are registered as P-23 and P-113. Of the two, P-113 was sensitive to ciprofloxacin and cefepime only, whereas the P-23 showed reduced sensitivity to ceftazidime, ciprofloxacin, and tigecycline. The genetic analysis of both isolates identified the presence of the\n                    <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                    <jats:sub>IND-like<\/jats:sub>\n                    gene, with similarity to IND-3 and IND-8 alleles. The IND-3 identified in the P-133 sample presented a single mutation at position T355G, which corresponds to a nonsynonymous substitution of the amino acid at position 119 (Ser\u2192Ala). The phylogenetic analysis of INDs showed lineages that are circulating in Asian and European countries. These results emphasize the need for effective preventive actions to avoid the dissemination of this type of pathogen in the hospital environment.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2018.0301","type":"journal-article","created":{"date-parts":[[2018,12,26]],"date-time":"2018-12-26T11:48:26Z","timestamp":1545824906000},"page":"781-786","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Molecular Characterization of\n                    <i>Chryseobacterium indologenes<\/i>\n                    with Multidrug Resistance in the Brazilian Amazon Region"],"prefix":"10.1177","volume":"25","author":[{"given":"Ellen Albuquerque de","family":"Freitas","sequence":"first","affiliation":[{"name":"Universidade do Estado do Amazonas\u2014UEA, Brazil."},{"name":"Laborat\u00f3rio de Gen\u00f4mica, Funda\u00e7\u00e3o de Hematologia e Hemoterapia do Amazonas\u2014HEMOAM, Manaus, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William Antunes","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Universidade do Estado do Amazonas\u2014UEA, Brazil."},{"name":"Laborat\u00f3rio de Bacteriologia, Funda\u00e7\u00e3o de Dermatologia Tropical e Venereologia Alfredo da Matta\u2014FUAM, Manaus, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto Alexandre Alves Barbosa","family":"Filho","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Diagn\u00f3stico Molecular, Universidade Federal do Amazonas, Manaus, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cintia Mara Costa de","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Diagn\u00f3stico Molecular, Universidade Federal do Amazonas, Manaus, Brazil."},{"name":"BIONORTE, Universidade Federal do Amazonas, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anamika","family":"Dhyani","sequence":"additional","affiliation":[{"name":"Universidade do Estado do Amazonas\u2014UEA, Brazil."},{"name":"Laborat\u00f3rio de Gen\u00f4mica, Funda\u00e7\u00e3o de Hematologia e Hemoterapia do Amazonas\u2014HEMOAM, Manaus, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucyane Mendes","family":"Silva","sequence":"additional","affiliation":[{"name":"Universidade do Estado do Amazonas\u2014UEA, Brazil."},{"name":"Laborat\u00f3rio de Gen\u00f4mica, Funda\u00e7\u00e3o de Hematologia e Hemoterapia do Amazonas\u2014HEMOAM, Manaus, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nelson Abrahim","family":"Fraiji","sequence":"additional","affiliation":[{"name":"Universidade do Estado do Amazonas\u2014UEA, Brazil."},{"name":"Laborat\u00f3rio de Gen\u00f4mica, Funda\u00e7\u00e3o de Hematologia e Hemoterapia do Amazonas\u2014HEMOAM, Manaus, Brazil."},{"name":"Departamento de Atendimento a Pacientes, Funda\u00e7\u00e3o de Hematologia e Hemoterapia do Amazonas, HEMOAM, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina Motta","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Universidade do Estado do Amazonas\u2014UEA, Brazil."},{"name":"Laborat\u00f3rio de Gen\u00f4mica, Funda\u00e7\u00e3o de Hematologia e Hemoterapia do Amazonas\u2014HEMOAM, Manaus, Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2019,5,30]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01397-13"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gdata.2015.12.024"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00083-07"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01607-08"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1139\/cjm-2015-0800"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/ccr3.753"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13099-016-0130-4"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmii.2014.04.004"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.1.445-448.2004"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmmcr.0.001008"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13104-016-2022-6"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.4103\/0255-0857.99510"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.4.2021-2023.2005"},{"key":"e_1_3_3_15_2","first-page":"633","article-title":"Sepsis grave secundaria a bacteriemia por Chryseobacterium indologenes. 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