{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T12:36:38Z","timestamp":1790339798981,"version":"4.1.0"},"reference-count":49,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2019,4,1]],"date-time":"2019-04-01T00:00:00Z","timestamp":1554076800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Microbial Drug Resistance"],"published-print":{"date-parts":[[2019,4,1]]},"abstract":"<jats:p>\n                    The worldwide dissemination of multidrug-resistant (MDR) Enterobacteriaceae is a major public health issue. The aim of this study was to investigate the prevalence of MDR\n                    <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                    (MDR-EC) isolates\n                    <jats:italic toggle=\"yes\">,<\/jats:italic>\n                    in inpatients\/outpatients with urinary tract infections at S\u00e9tif University Hospital (Algeria). Bacterial cultures were obtained from 426 of the 3,944 urine samples collected from January 2015 to February 2017. Among these cultures, 215\n                    <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                    isolates were identified by mass spectrometry, and 38 (17.7%) were MDR-EC (disk diffusion method): 36 produced only extended-spectrum \u03b2-lactamases (ESBL), one ESBL and a carbapenemase, and one only a cephalosporinase (double-disk synergy test). Multiplex PCR and sequencing analyses showed that 37 ESBL-producing isolates harbored genes encoding CTX-M enzymes (CTX-M-15 in 33 isolates, 89.19%; and CTX-M-14 group in four isolates, 10.81%). One CTX-M-15-producing isolate co-expressed also an OXA-48-like carbapenemase. Phylogenetic group analysis of the 37 ESBL-producing and 178 non-ESBL-producing isolates indicated that the most common phylogenetic group was B2 (54.05% of ESBL-producing and 48.31% of non-ESBL-producing isolates), followed by A and D for ESBL-, and by B1, A, and F for non-ESBL-producing isolates. This is the first report highlighting the presence of MDR-EC isolates that produce both CTX-M and OXA-48-like enzymes in S\u00e9tif, Algeria.\n                  <\/jats:p>","DOI":"10.1089\/mdr.2018.0314","type":"journal-article","created":{"date-parts":[[2019,1,24]],"date-time":"2019-01-24T11:19:49Z","timestamp":1548328789000},"page":"386-393","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":27,"title":["High Prevalence of Multidrug-Resistant\n                    <i>Escherichia coli<\/i>\n                    in Urine Samples from Inpatients and Outpatients at a Tertiary Care Hospital in S\u00e9tif, Algeria"],"prefix":"10.1177","volume":"25","author":[{"given":"Larbi Zakaria","family":"Nabti","sequence":"first","affiliation":[{"name":"Laboratoire de Valorisation des Ressources Biologiques et Naturelles, FSNV, Universit\u00e9 de S\u00e9tif 1, S\u00e9tif, Alg\u00e9rie."},{"name":"D\u00e9partement des Sciences 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France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manon","family":"Lounnas","sequence":"additional","affiliation":[{"name":"CHU de Montpellier, Laboratoire de Bact\u00e9riologie, Universit\u00e9 de Montpellier, Montpellier, France."},{"name":"UMR MIVEGEC IRD-CNRS-Universit\u00e9 de Montpellier, IRD, Montpellier, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9r\u00f4me","family":"Solassol","sequence":"additional","affiliation":[{"name":"D\u00e9partement Bio-pathologie cellulaire et tissulaire des tumeurs, CHU de Montpellier, Universit\u00e9 de Montpellier, Montpellier, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Noelle","family":"Didelot","sequence":"additional","affiliation":[{"name":"CHU de Montpellier, Laboratoire de Bact\u00e9riologie, Universit\u00e9 de Montpellier, Montpellier, 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